Effective pressure and treatment duration of high hydrostatic pressure to prepare melanoma vaccines

Abstract

Current therapeutic methods for melanoma have numerous limitations, and thus the improvement of such treatment methods are essential. One possible option is the vaccination of autologous inactivated tumor cells. The primary indispensable principles of a cell-based melanoma vaccine include: i) Entire inactivation of melanoma cells; ii) retaining the immunogenicity of melanoma cells; and iii) adherence to laws and ethical guidelines. However, traditional methods for the production of the vaccine, such as ultrasonic, chemotherapeutics and freeze-thawing, have some juridical or therapeutic constraints. Therefore, the present study used high hydrostatic pressure (HHP) to inactivate malignant cells, and treated B16-F10 tumor cells with different pressures (≥50 MPa) and different durations (≥1 min). It was identified that tumor cells in vitro lost their proliferative ability, but retained their immunogenicity following treatment. Furthermore, the vaccination of the melanoma cells significantly suppressed their oncogenesis. Collectively, the present results suggest that HHP treatment may be an economically viable and effective measure to develop a melanoma vaccine, when pressure was ≥200 MPa and the treatment duration was ≥30 min.

Cellular transfection using rapid decrease in hydrostatic pressure

“…High hydrostatic pressure is successfully employed as a means of sterilizing sensitive foodstuffs in order to minimize alterations in flavor, body, texture, and nutrients 1 , 3 . More recently it has been used to decellularize biological materials for which traditional methods would be inappropriate and to generate peptides for anti-tumor vaccines 1 , 4 – 6 , 9 , 36 . In the current study, we have examined the use of sudden depressurization from pressures in the range of 60–100 MPa to induce gene transfection in difficult to transfect primary cells; we have termed this method Pressure-jump-poration (PJP).…”

Extracellular Matrix Cues Regulate Mechanosensing and Mechanotransduction of Cancer Cells

“…Cell death due to apoptosis takes place at a pressure of about 200 MPa [ 71 ], and cell necrosis develops at a pressure of more than 300 MPa [ 64 , 71 , 72 ]. HHP has been characterized to inactivate B16-F10 melanoma cells at various pressures (≥50 MPa) and for several time periods (≥1 min) [ 73 ]. Their findings indicate that HHP can be an efficacious melanoma vaccine generation tool when the pressure is ≥200 MPa and the duration of treatment is ≥30 min.…”

“…Cell death due to apoptosis takes place at a pressure of about 200 MPa [71], and cell necrosis develops at a pressure of more than 300 MPa [64,71,72]. HHP has been characterized to inactivate B16-F10 melanoma cells at various pressures (≥50 MPa) and for several time periods (≥1 min) [73].…”

Research and application of hydrostatic high pressure in tumor vaccines (Review)

“…Cell death occurs through apoptosis at pressures of approximately 200 MPa ( 47 ), and cell necrosis occurs at pressures >300 MPa ( 23 , 24 , 47 ). Liu et al used HHP to inactivate B16-F10 melanoma cells at different pressures (≥50 MPa) and for different durations (≥1 min) ( 48 ). Their results suggested that HHP may be an effective measure for the preparation of melanoma vaccines when the pressure was ≥200 MPa and the treatment duration was ≥30 min.…”

“…Liu et al used HHP to inactivate B16-F10 melanoma cells at different pressures (≥50 MPa) and for different durations (≥1 min) (48). Their results suggested that HHP may be an effective measure for the preparation of melanoma vaccines when the pressure was ≥200 MPa and the treatment duration was ≥30 min.…”