Cellular transfection using rapid decrease in hydrostatic pressure
Shudi Huang,
Nan Ji Suo,
Tyler R. Henderson
et al.
Abstract:Of all methods exercised in modern molecular biology, modification of cellular properties through the introduction or removal of nucleic acids is one of the most fundamental. As such, several methods have arisen to promote this process; these include the condensation of nucleic acids with calcium, polyethylenimine or modified lipids, electroporation, viral production, biolistics, and microinjection. An ideal transfection method would be (1) low cost, (2) exhibit high levels of biological safety, (3) offer impr… Show more
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