High-fructose and high-fat diet-induced disorders in rats: impact on diabetes risk, hepatic and vascular complications

Abstract

BackgroundAs a result of the increased consumption of sugar-rich and fatty-products, and the increase in preference for such products, metabolic disorders are becoming more common at a younger age. Fructose is particularly used in prepared foods and carbonated beverages. We investigated the impact of regular consumption of fructose, in combination or not with fatty food, on the onset of metabolic syndrome and type 2 diabetes (T2D). We evaluated the metabolic, oxidative, and functional effects on the liver and blood vessels, both related to diabetes complications.MethodsHigh-fat diet (HFD), high-fructose beverages (HF) or both (HFHF) were compared to rats fed with normal diet (ND) for 8 months to induce T2D and its metabolic, oxidative, and functional complications. Metabolic control was determined by measuring body weight, fasting blood glucose, C-peptide, HOMA2-IR, leptin, and cholesterol; oxidative parameters were studied by lipid peroxidation and total antioxidant capacity in plasma and the use of ROS labelling on tissue. Histological analysis was performed on the liver and endothelial function was performed in main mesenteric artery using organ-baths.ResultsAfter 2 months, HFHF and HFD increased body weight, leptin, HOMA2-IR associated to steatosis, oxidative stress in plasma and tissues, whereas HF had only a transient increase of leptin and c-peptide. Only HFHF induced fasting hyperglycaemia after 6 months and persistent hyperinsulinaemia and fasting hyperglycaemia with complicated steatosis (inflammation and fibrosis) after 8 months. HFHF and HFD induced endothelial dysfunction at 8 months of diet.ConclusionsSix months, high fat and high carbohydrate induced T2D with widespread tissues effects. We demonstrated the role of oxidative stress in pathogenesis as well as in complications (hepatic and vascular), reinforcing interest in the use of antioxidants in the prevention and treatment of metabolic diseases, including T2D.

Exercise improves high fat diet-impaired vascular function

“…As anticipated, HFD increased the level of aortic ROS, serum leptin and resistin, which further prompted aortic tissue maladaptive immune response and release of proinflammatory cytokines, including MCP1, IL6 and IL8. It has been demonstrated that HFD and high-fructose beverages increased body weight, leptin, inflammation and insulin resistance that are associated with steatosis, and oxidative stress in plasma and tissues, indicating that oxidative stress and inflammation are important contributors to the pathogenesis of CVD (20,21).…”

Distribution of ABO and Rhesus (Rh) blood group antigens in male type 2 diabetes mellitus patients in Hail region of Saudi Arabia: High incidences of diabetes mellitus in males with B+ blood type

“…Recently there has been a lot of debate about the importance of diet [12,13] and importance of blood group antigens and their association in causing certain diseases [11]. Since diabetes is also prevalent in Saudi Arabia, we decided to investigate the frequency of distribution of ABO and Rhesus (Rh) antigen among the T2DM patients and the We also analyzed the data of 342 diabetes II male and female patients (who registered at King Khalid Hospital).…”

Lanthanum Chloride Inhibits LPS Mediated Expressions of Pro-Inflammatory Cytokines and Adhesion Molecules in HUVECs: Involvement of NF-κB-Jmjd3 Signaling

“…Vascular inflammation plays important roles in various diseases including cardiovascular diseases and diabetes [1-3]. It is well-established that pro-inflammatory cytokines and adhesion molecules contribute to these diseases.…”