High frequency plant regeneration from the mature seeds of Garcinia indica

Abstract

A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg dm -3 N 6 -benzyladenine and 1 mg dm -3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm -3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.

Micropropagation of yellow mangosteen: a valuable endemic tree of India

“…80-100 % of explants developed 1-3 shoots on medium supplemented with KN, whereas TDZ-supplemented medium was not beneficial in shoot bud differentiation and explants remained green even after subculturing to fresh medium. The superiority of BAP on shoot bud induction was previously reported for G. mangostana (Goh et al 1990;Huang et al 2000) and G. indica (Malik et al 2005;Baskaran and Krishnan 2011). Similar effect of BAP in inducing multiple shoots has been reported for tree species such as F. limonia (Hiregoudar et al 2003), V. trifolia (Hiregoudar et al 2006) and Salix pseudolasigyne (Park et al 2008).…”

“…1c). Malik et al (2005), Baskaran and Krishnan (2011) observed similar response of swelling of seed explants along with shoot bud differentiation on the medium supplemented with growth regulators in G. indica. Development of somatic embryos was reported by Thengane et al (2006) on immature seed explants of G. indica.…”

“…The elongated shoots were excised and the explants were cultured again onto fresh medium for elongation of the remaining shoots. Baskaran and Krishnan (2011) reported that removal of the elongated shoots at regular intervals was necessary to enable the elongation of smaller shoots in G. indica.…”

“…Micropropagated planting stock is useful for re-forestation programmes, gardening and the conservation of germplasm. In vitro plant regeneration has been accomplished for many tree species including Feronia limonia (Hiregoudar et al 2003), Vitex trifolia (Hiregoudar et al 2006), Salix pseudolasiogyne (Park et al 2008), Garcinia indica (Baskaran and Krishnan 2011;Deodhar et al 2008;Thengane et al 2006;Malik et al 2005), Garcinia mangostana (Goh et al 1990;Huang et al 2000).…”

In Vitro Propagation of Garcinia livingstonei T. Anderson (African Mangosteen) a Woody Tree Species through the Culture of Matured Seed Segments

“…The propagation of this species through seeds is difficult, its seeds have a short shelf life and propagation through softwood grafting is not effective. There are many reports on the regeneration of different Garcinia species through in vitro micropropagation such as Garcinia xanthochymus [10], G. mangostana [11,12], and G. indica [13][14][15]. However, there are no regeneration protocols for the mass multiplication and conservation of Garcinia livingstonei, therefore the present investigation was taken up to develop an efficient in vitro propagation through mature seed explants.…”