High frequency plant regeneration and accumulation of tropane alkaloids in regenerated plants of Scopolia parviflora

Kim, Yong Duck; Min, Ji Yun; Kim, Won Jung; Kang, Young Min; Moon, Hyun Kyung; Lee, Cheul Ho; Prasad, D.; Choi, Myung Suk

doi:10.1007/s11627-007-9073-2

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…A BA concentration higher than 17.7 lM did not improve shoot proliferation, but reduced the percentage of bud break to a greater extent. Cytokinins are most critical for shoot induction and elongation in several systems including medicinal plants such as Clitoria ternatea (Rout 2005), Scopolia parviflora (Kim et al 2008) and Melothria maderaspatana (Baskaran et al 2009). Moreover, it is well known that BA promotes shoot formation in vitro.…”

Section: Culture Of Nodal Cuttingsmentioning

confidence: 99%

In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

Thomas

Hoshino

2010

Acta Physiol Plant

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Justicia gendarussa is a valuable medicinal plant and various parts of this plant are pharmaceutically used for the treatment of different diseases. In vitro regeneration of shoot buds was obtained from culture of nodal cuttings as well as shoot regeneration from callus. The nodal cuttings differed in shoot proliferation in terms of percentage of explants that responded and average shoot length with various concentrations (4.4, 8.9, 13.3, 17.7, 22.2 lM) of 6-benzyladenine (BA), kinetin (Kn) and thidiazuron. In all treatments, one shoot was invariably present. Optimum 87% of cultures responded with an average shoot length of 4.4 cm on Murashige and Skoog (MS) medium supplemented with 17.7 lM BA. Callus was induced from the mature leaf segments on MS medium supplemented with Kn (4.7, 13.9, 23.2 lM) alone or in combination with 2, 4-dichlorophenoxyacetic acid (2, 4-D; 2.3 lM, 4.5 lM). Optimum callus induction (78%) was obtained on MS medium supplemented with 14 lM Kn and 4.5 lM 2, 4-D. When the callus was subcultured on MS medium fortified with BA (8.9, 17.7, 26.6 lM) or Kn (9.3, 18.6, 27.9 lM) alone or in combination with a naphthalene acetic acid (NAA; 2.7, 5.4 lM), shoot regeneration was obtained. The highest response (92%) was observed on MS medium containing 17.7 lM BA and 5.4 lM NAA. On this medium, an average number of 12.2 shoots were obtained per responding callus. The shoots obtained from callus and nodal cuttings were rooted with a frequency of 73% on MS medium augmented with 9.8 lM indole-3-butyric acid. The rooted shoots were successfully transplanted to soil and sand mixture (1:1) with 90% survival rate. The protocol standardized for shoot proliferation and regeneration in J. gendarussa from nodal cuttings and leaf-derived callus is suitable for micropropagation and conservation of this essential medicinal plant.

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Section: Culture Of Nodal Cuttingsmentioning

confidence: 99%

In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

Thomas

Hoshino

2010

Acta Physiol Plant

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“…Scopolamine content in de-differentiated tissues was high compared to that of hyoscyamine. As in an earlier study of Kim et al, the scoplolamine content in regenerated plants or organogenic calli was high compared to hyoscyamine. This result supposes that scopolamine biosynthesis is influenced by the cell differentiation process.…”

Section: Resultsmentioning

confidence: 85%

“…The adventitious roots of S. parviflora were induced from the rhizome of mature plants as reported . The organogenic and nonorganogenic calli were selected from adventitious root-derived calli …”

Section: Methodsmentioning

confidence: 99%

Production of Tropane Alkaloids during De-differentiation of Scopolia parviflora Calli

et al. 2010

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The production of tropane alkaloids during differentiation and de-differentiation of Scopolia parviflora calli was studied. Tropane alkaloid production drastically decreased during calli de-differentiation. Scopolamine (1) production decreased after 10 days of culture, whereas that of hyoscyamine (2) decreased during de-differentiation of root to calli. The production of 1 was enhanced in calli undergoing differentiation to shoot after 60 days of culture, reaching a maximum by 80 days. However, production of hyoscyamine in regenerated plants was lower. The expression level of hyoscyamine 6beta-hydroxylase (H6H), a key biosynthetic enzyme for tropane alkaloids, was significantly increased in 4-week-old calli. This study suggests that the biosynthesis of tropane alkaloids is regulated inversely in de-differentiating Scopolia parviflora calli.

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“…In plant tissue and cell culture analysis, hyoscyamine and scopolamine are measured by HPLC-DAD usually after separation on a RP18 column in an acetonitrile-buffer solvent system. Quantitation is mostly performed between 210 and 220 nm [72][73][74][75][76][77][78][79][80]. Control of pharmaceutical preparations containing atropine is possible by HPLC and DAD detection [81,82].…”

Section: Alkaloids In Plant Extracts and In Pharmaceutical Preparationsmentioning

confidence: 99%

Tropane alkaloid analysis by chromatographic and electrophoretic techniques: An update

Aehle

Dräger

2010

Journal of Chromatography B

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High frequency plant regeneration and accumulation of tropane alkaloids in regenerated plants of Scopolia parviflora

Cited by 6 publications

References 16 publications

In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus

Production of Tropane Alkaloids during De-differentiation of Scopolia parviflora Calli

Tropane alkaloid analysis by chromatographic and electrophoretic techniques: An update

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