High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocorms technique

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

“…Coconut water contains high levels of Z-type cytokinin (trans-zeatin riboside, trans-zeatin O-glucoside, dihydrozeatin O-glucoside, trans-zeatin, dihydrozeatin, trans-zeatin riboside-5 -monophosphate), iP-type cytokinin (N6-isopentenyladenine), kinetin, kinetin riboside and ortho-topolin in addition to sugars, vitamins, minerals and amino acids (Yong, Ge, Ng, & Tan, 2009;Tan, Cheng, Bhat, Rusul, & Easa, 2014). Murdad et al, (2006) reported that the production of PLBs in Phalaenopsis gigantean is improved with the addition of coconut water in the culture medium. The PLB and callus induction in Cymbidium hybrids is also promoted by the coconut water in the culture media (Teixeira da Silva & Tanaka, 2006).…”

The Effects of Different Media, Sucrose Concentrations and Natural Additives on Plantlet Growth of Phalaenopsis Hybrid 'Pink'

“…The genus of Phalaenopsis is a monopodial orchid; therefore, mass vegetative propagation is limited for this orchid [6,16] . Tissue culture techniques have been widely employed for rapid and large-scale propagation of this species [8,[17][18] . However, the effectiveness of micropropagation varies among plant species, types of explants and culture media [18] .…”

CRESCIMENTO IN VITRO DE DENDROBIUM PHALAENOPSIS C.V. “MADAME POMPADOUR” CULTIVADAS EM DIFERENTES MEIOS DE CULTURA E NÍVEIS DE pH

“…O pH de meios de cultivo se modifica à medida que diferentes íons são absorvidos e os produtos metabólicos são excretados para o meio, fato este principalmente causado pela absorção diferencial do amônio e do nitrato (MUrDAD et al, 2006). Mesmo que o nível de pH 5,8 seja amplamente utilizado em biofábricas para a reprodução de variadas orquídeas, sabe-se que para o cultivo in vitro de Dendróbios, o melhor nível de pH para o desenvolvimento de espécies é 6,0 (PEDrOSO- DEMOrAES et al, 2010).…”