High–frequency in vitro plantlet regeneration from apical bud as a novel explant of Carum copticum L.

Salehi, Mansoureh; Hosseini, Bahman; Jabbarzadeh, Zohreh

doi:10.12980/apjtb.4.2014c529

Cited by 11 publications

(8 citation statements)

References 18 publications

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“…6a, b). The variation in proliferation, shoot and root regeneration response might be due to the influence of growth regulators and hormones on growing and developing callus (Salehi et al 2014), genotypic and phenotypic characters of the mother plant (Benderradji et al 2012). Due to the time necessity for whole plant regeneration, many authors (Ho and Vasil 1983; Ahloowalia and Maretzki 1983) did not mention clearly the process of plant regeneration.…”

Section: Resultsmentioning

confidence: 99%

Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers

et al. 2017

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The aim of this study was to produce sugarcane plantlets from cell suspension culture and study its genetic fidelity using molecular markers. The study was carried out using sugarcane varieties Co 86032 and Q117. Callus cultures of both the varieties were optimized using six different callus induction media. After screening the growth response of callus on six different callus induction media, it was observed that medium no. VI supplemented with 500 mg l−1 of each PVP, Casein hydrolysate and MES buffer showed high amounts of callus in Co 86032 (79.66 ± 0.44%) and Q117 (82.83 ± 1.69%). Addition of PEG 8000 at 2.5% to this medium had a profound impact on inducing somatic embryogenesis in Co 86032 (54.66 ± 1.76%) and Q117 (66.66 ± 2.60%) as compare to control (24.33 ± 1.76%) and (27.33 ± 2.73%), respectively. Cell suspension cultures were established by culturing embryogenic calli in liquid medium showed well established suspension cultures with fever cell aggregates. There was negligible cell division during initial 2 days of incubation and cell count increased rapidly between 2 and 8 days. Further incubation beyond 8 days resulted in a decrease in cell viability. Enhanced callus proliferation in Q117 while enhanced shoot regeneration in Co 86032 was observed from cell suspension culture. The clonal fidelity of in vitro regenerated plants was assessed by using RAPD and ISSR markers. Analysis of the ten RAPD markers indicated that 90.48 and 86.95% true-to-type regenerated plantlets in Co 86032 and Q117, respectively. However, in the ISSR markers, Co 86032 did not show any polymorphism and in the Q117, 92.18% true-to-type plantlets were found. These results confirmed that somaclonal variation occurs during the process of indirect organogenesis and RAPD and ISSR marker based molecular analysis is a suitable method for an early detection of variation in sugarcane.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-016-0579-3) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers

et al. 2017

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“…e.g. plant regeneration from callus of T. ammi (Jasrai et al 1992, Sehgal andAbbas 1994), callus induction and regeneration from apical bud of Carum copticum L. (Salehi et al 2014), direct shoot micropropagation system in T. ammi by using seed as explants (Koca and Aasim 2015), direct somatic embryogenesis from cotyledon and cotyledonary node explants in T. ammi (Purohit and Kothari 2007).…”

Section: Resultsmentioning

confidence: 99%

“…The seeds were washed thoroughly under running tap water for 30 min. The soaked seeds were treated with 70% ethyl alcohol for 1 min and 0.5% (v/v) sodium hypochlorite for 10 min and then rinsed three times with sterile distilled water (Salehi et al 2014). The surface sterilized seeds were cultured on MS medium and incubated at 26 ± 2°C in darkness for germination.…”

Section: Methodsmentioning

confidence: 99%

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In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Teymourian

Ebrahimi

Tohidfar

et al. 2017

Plant Tissue Cult. & Biotech.

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The effect of explant sources and plant growth regulators on callus induction and plantlet regeneration of Trachyspermum copticum were explored. Different explants including hypocotyl, cotyledonary node and leaf were cultured on MS supplemented with different combinations and concentrations of plant growth regulators including 2,4-D (0.2-3 0.5 mg/l), NAA (2 mg/l), BAP (1-3 mg/l), Kn (0.5 mg/l) and IAA (0.8 mg/l). The best response for callus induction (100%) as well as quality was observed from cotyldonary node segments cultured on MS supplemented with 2, 4-D at 1 mg/l in combination with Kn at 0.5 mg/l. Calli derived from various explants were subcultured on shoot induction media with different compositions and concentrations of medium. MS without any plant growth regulator promoted the highest frequency of shoot regeneration (100%) and also mean number of developed shoots per explants (3.8) showed the same result. Regenerated shoots were then rooted on three-fourth strength MS with 75% efficiency after 30 days.

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“…tissue culture technique to ensure its restoration. Tissue culture methods offer highly efficient tools for germplasm conservation and mass multiplication of many threatened plant species (Salehi et al 2014;Ozel et al 2015). Sreedevi et al (2013) and Thangavel et al (2014a) have previously reported successful organogenesis from Plectranthus barbatus using leaf explants.…”

Section: Introductionmentioning

confidence: 99%

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

Thaniarasu

Kumar

Rao

2016

Physiol Mol Biol Plants

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The present study describes the plant propagation via indirect organogenesis from in vitro derived leaf and internode explants of Plectranthus bourneae, an endemic plant to south India. Leaf and internodal explants successfully callused on Murashige and Skoog medium (MS) supplemented with different concentrations of auxins [2,4-D (2, 4-dichlorophenoxyacetic acid), NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid) and PIC (Picloram); 0.1-2.0 mg/l] in combination with BA (6-benzyladenine) (0.5 mg/l). Maximum callus induction (98 %) was achieved from leaf explant followed by internodal explant (89 %) at 1.0 mg/l NAA, 0.5 mg/l BA. Leaf derived callus showed better shoot regeneration (29.71 shoots) on MS medium containing 1.0 mg/l KN (kinetin), 0.7 mg/l NAA, and 50 mg/l CH (casein hydrolysate) followed by internodal callus (19.71). A maximum of 19.14 roots/shoot was observed at 1.0 mg/l IBA. The rooted plantlets were successfully hardened and transferred to greenhouse condition with 80 % survival. This system could be utilized for large-scale multiplication of P. bourneae by tissue culture.

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High–frequency in vitro plantlet regeneration from apical bud as a novel explant of Carum copticum L.

Cited by 11 publications

References 18 publications

Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers

Plant regeneration from cell suspension culture in Saccharum officinarum L. and ascertaining of genetic fidelity through RAPD and ISSR markers

In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

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