High-frequency clonal propagation of Curcuma angustifolia ensuring genetic fidelity of micropropagated plants

Jena, Sudipta; Ray, Asit; Sahoo, Ambika; Sahoo, Suprava; Kar, Basudeba; Panda, Pratap Chandra; Nayak, Sanghamitra

doi:10.1007/s11240-018-1480-z

Cited by 34 publications

(11 citation statements)

References 46 publications

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“…1, indicating that in vitro raised V. amurensis 'Cheongsan' preserved genetic stability without any notable morphological differences. Our result is consistent with other findings showing no genetic variations from in vitro raised plantlets of several species of plants (Purohit et al, 2016;Jena et al, 2018;Behera et al, 2019).…”

Section: Analysis Of Genetic Stability Reported By Issr Markersupporting

confidence: 94%

Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

Kwon

Park

Kim

et al. 2019

Not Bot Horti Agrobo

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Vitis amurensis ‘Cheongsan’ is a plant with high economic value in both medical and agricultural applications. However, its utilization has been restricted owing to difficulties encountered when applying traditional mass propagation methods, requiring instead application of in vitro propagation methods for their mass scale production. Hence, this study was conducted to find the optimal plant growth regulators for shoot multiplication and root induction during in vitro propagation. Among the three cytokinins used at multiple concentrations for culture initiation and shoot multiplication, the most positive response was found with MS medium containing 5.0 μM 6-benzyladenine (BA), compared to more modest responses from other types of cytokinin, such as kinetin (KIN) and thidiazuron (TDZ). For root induction, medium supplemented with α-naphthaleneacetic acid (NAA) produced a callus and inhibited shoot growth in explants, whereas indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) did not create any significant problems, but did display differences in root induction efficiencies. Generally, root induction responses with IBA were better that those with IAA. The maximum rooting rates were observed without callus formation and no shoot growth inhibition from explants grown on media supplemented with 0.67 μM IBA. Further, inter-simple sequence repeat (ISSR) analyses revealed that micropropagated plantlets generated in medium supplemented with 5.0 μM BA and 0.67 μM IBA did not lead to genetic variation. Therefore, the application of the in vitro propagation method developed in this study could be used on a commercial scale and will offer opportunities to strengthen the industrial use of V. amurensis ‘Cheongsan’.

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Section: Analysis Of Genetic Stability Reported By Issr Markersupporting

confidence: 94%

Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

Kwon

Park

Kim

et al. 2019

Not Bot Horti Agrobo

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“…The good news is that researchers are beginning to improve local cultivars and finding suitable methods of micropropagation of these Curcuma species, for example, C. angustifolia Roxb. [47].…”

Section: Recent Publications On the Ginger Familymentioning

confidence: 99%

Polyploidy in the Ginger Family from Thailand

Anamthawat‐Jónsson¹,

Umpunjun²

2020

Chromosomal Abnormalities

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Polyploidy is common in the ginger family Zingiberaceae. The aims of the present paper are (1) to provide a general introduction on species diversity with emphasis on conservation; (2) to highlight the human-use significance of this family, focusing on the two major genera, Zingiber (ginger) and Curcuma (turmeric); (3) to present chromosome number data from 45 natural and cultivated Curcuma taxa from Thailand, of which polyploids are predominant; and (4) to describe our own work on cytotaxonomy of selected Thai Curcuma species. We obtained somatic chromosome numbers from root tips and analysed meiotic chromosome behaviour from flowers. We also used the molecular cytogenetic method of ribosomal gene mapping on chromosomes to infer mechanism of polyploidization and reveal genomic relationships among closely related species. The main results of our cytogenetic studies include the following. The most sought-after medicinal Curcuma cultivars growing on a large-scale basis are secondary triploids, so as taxa in natural habitats that are harvested for local utilisation. These triploids are sexually deficient, due to meiotic pairing abnormalities, but they are propagated asexually via rhizomes. The ribosomal mapping results indicate natural triploidization process via hybridisation, either within populations or across the species boundaries.

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“…Among the cytokinins evaluated, 6-BA yielded the best explant response (60.2%), followed by TDZ (48.5%) and 6-KN (43.6%). Advantages of 6-BA on plant regeneration in vitro have been disclosed for K. parviflora [ 20 , 21 ] and other Zingiberaceae members such as Curcuma angustifolia [ 47 ], H. coronarium [ 44 ], and K. galanga [ 43 ]. However, a significant difference in the number of roots was not found among cytokinins ( Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Several works have shown that a combination of PGRs can boost the regeneration of multiple shoots for Zingiberaceae members [ 43 , 44 , 46 , 47 ]. Chithra et al [ 43 ] used a combination of 11.4 µM silver nitrate, 8.8 µM 6-BA, and 2.46 µM Indole-3-butyric acid (IBA) to induce maximal axillary buds (8.3) and roots (6.7) for K. galanga .…”

Section: Resultsmentioning

confidence: 99%

“…Parida et al [ 46 ] used a combination of 14.1 µM 6-KN and 2.7 µM Naphthalene-1-acetic acid (NAA) to induce 9.5 axillary shoots and 4.5 roots for G. marantina . Jena et al [ 47 ] used 135.7 µM adenine sulfate, 13.3 µM 6-BA, and 5.7 µM Indole-3-acetic acid (IAA) to obtain higher shoots (14.1) and roots (7.6) for C. angustifolia . In the present study, rhizome bud explants were placed on OM (optimal medium: MS plus 8 µM 6-BA) combined with other PGRs ( Table 5 ) to produce roots and shoots within 14 days of cultivation.…”

Section: Resultsmentioning

confidence: 99%

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Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

Park

Kim

et al. 2021

Plants

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This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of Kaempferia parviflora to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L−1 AgO NPs for 60 min eliminated contamination without affecting the survival of explants. The number of shoots (12.2) produced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 µM of 6-Benzyladenine (6-BA) and 0.5 µM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 µM of Indole-3-butyric acid (IBA). A survival rate of 98% was attained when plantlets of K. parviflora were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to determine the chemical profile of K. parviflora leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse-grown) plantlets of K. parviflora. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase inhibitory abilities. These results suggest that leaves of K. parviflora, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds.

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High-frequency clonal propagation of Curcuma angustifolia ensuring genetic fidelity of micropropagated plants

Cited by 34 publications

References 46 publications

Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

Evaluation of Genetic Stability and Effects of Plant Growth Regulators for in vitro Propagation of Underutilized Vitis amurensis ‘Cheongsan’

Polyploidy in the Ginger Family from Thailand

Establishment of a Rapid Micropropagation System for Kaempferia parviflora Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities

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