High Frequency and Diversity of Species C Enteroviruses in Cameroon and Neighboring Countries

Sadeuh-Mba, Serge Alain; Bessaud, Maël; Massenet, D; Joffret, Marie-Line; Endegue, Marie-Claire; Njouom, Richard; Reynes, Jean-Marc; Rousset, Dominique; Delpeyroux, Françis

doi:10.1128/jcm.02119-12

Cited by 98 publications

(180 citation statements)

References 60 publications

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“…Though the strategy suggested above might lengthen the storage time of enterovirus isolates recovered and our ability to revisit such isolates for re-analysis, prior subjection to cell culture might result in cell line bias and consequently, influence our perception of the enterovirus diversity landscape of the sample as previously shown [17][18][19][20][21][22]. Subsequent to the completion of this study, we used the same algorithm described in this study to directly screen faecal suspensions from AFP cases and their corresponding isolates on RD cell line.…”

Section: Discussionmentioning

confidence: 99%

The Impact of a Panenterovirus Vp1 Assay on Our Perception of the Enterovirus Diversity Landscape of a Sample

Adeniji

Faleye

Adewumi

et al. 2016

JHVRV

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The number of independent emergence of recombinant circulating vaccine derived poliovirus serotype 2 (cVDPV2) lineages and the magnitude of the outbreak in Nigeria demonstrates the significance of enterovirus co-infection and its preponderance in the country. Despite this, besides polioviruses, little or no attention is given to enterovirus co-infections. More recently, a reversetranscriptase semi-nested polymerase chain reaction (RT-snPCR) assay for the direct detection of enteroviruses from clinical specimen was added to the WHO recommended protocols for enterovirus surveillance. We previously showed that primers 292 and 222 (for which AN89 and AN88, respectively, are consensus degenerate hybrid oligonucleotide primers [CODEHOP] versions) mask the presence of enterovirus co-infections. We therefore ask whether the primers AN89 and AN88 used in this recently recommended RT-snPCR protocol, like primers 292 and 222, will also mask the presence of enterovirus co-infections. RNA was extracted from 30 archived samples (both clinical specimen and cell culture isolates) and the VP1 gene amplified using the WHO recommended RTsnPCR assay and modifications that included the primers 187, 188 and 189 for which primer 292 (and consequently AN89) is a consensus. Amplicons were sequenced and isolates identified. Our results showed that primers AN89 and AN88 also mask enterovirus co-infection and inclusion of the primers 187, 188 and 189 allowed the resolution of such mixed isolates. Consequently, expanding the recommended RT-snPCR protocol to include primers 187, 188 and 189 will enable us better detect enterovirus co-infection.

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Section: Discussionmentioning

confidence: 99%

The Impact of a Panenterovirus Vp1 Assay on Our Perception of the Enterovirus Diversity Landscape of a Sample

Adeniji

Faleye

Adewumi

et al. 2016

JHVRV

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“…Les résultats obtenus jusqu'à maintenant, concernant quatre pays, indiquent que les HEV-C circulent très peu en Roumanie et en Tunisie [22,23]. En revanche, des fréquences relativement élevées ont été mises en évidence en République Centrafricaine et au Cameroun [24,25]. Au Cameroun, nous nous sommes intéressés plus particulièrement à la circulation et à la diversité génétique des HEV circulant chez les enfants sains dans l'extrême nord du pays, une région limitrophe des états du Nigéria où l'émergence de cPVDV est récurrente [21].…”

Section: Le Réseau International Des Instituts Pasteurunclassified

Éradication de la poliomyélite et émergence de poliovirus pathogènes dérivés du vaccin

Delpeyroux

Colbère-Garapin

Razafindratsimandresy

et al. 2013

Med Sci (Paris)

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“…In Africa; several countries were affected including Madagascar in 2001-2002 and 2005 [11], in the Northern states of the Nigerian Federation (2005-2015), in Cameroon, Chad and the Democratic Republic of Congo (DRC) [9]. However, the number of countries with circulation of indigenously emergent cVDPVs decreased from 18 countries in 2000 to four (Pakistan, Nigeria, Madagascar, and South Sudan) in 2015.…”

Section: Archives Of Medicine Issn 1989-5216mentioning

confidence: 99%

“…Vol.9 No.4:5 enteroviruses as they spread through a community [8,9]. But in case of the population which is fully immunized against poliomyelitis, it will be protected against the spread of both WPV and cVDPVs [9].…”

Section: Archives Of Medicine Issn 1989-5216mentioning

confidence: 99%

“…Poliovirus serotyping was performed by real-Time Reverse Transcriptase Polymerase Chain Reaction using cell culture supernatants according to the protocol recommended by WHO [8,9]. The Intratypic differentiation (ITD) method was carried out using PVs group, serotype-specific, and Sabin strain-specific primers sets, in order to determine whether the PVs isolated are from the wild type, sabin strain or cVDPV and the serotype involved [13].…”

Section: Intratypic Differentiation By Real Time Polymerase Chain Reamentioning

confidence: 99%

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Disease Trends and Characteristics of Circulating Vaccine derived Poliovirus Isolated in the National Reference Polio Laboratory from the Democratic Republic of Congo

Kavunga¹,

Simulundu²,

Ahuka³

et al. 2017

Arch Med

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Background: Despite the fact that wild poliovirus (WPV) circulation has not been detected for the past six years, cases of poliomyelitis caused by pathogenic circulating vaccine-derived polioviruses (cVDPVs) have been identified in the Democratic Republic of Congo (DRC). Most cVDPVs implicated in polio outbreaks are recombinants between mutated strains of the oral poliovaccine (OPV) and other human enteroviruses (HEV) of species C (HEV-C).The aim of this study was to determine the disease trend and the genetic makeup of cVDPVs strains isolated in DRC from 2008 to 2016.

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High Frequency and Diversity of Species C Enteroviruses in Cameroon and Neighboring Countries

Cited by 98 publications

References 60 publications

The Impact of a Panenterovirus Vp1 Assay on Our Perception of the Enterovirus Diversity Landscape of a Sample

The Impact of a Panenterovirus Vp1 Assay on Our Perception of the Enterovirus Diversity Landscape of a Sample

Éradication de la poliomyélite et émergence de poliovirus pathogènes dérivés du vaccin

Disease Trends and Characteristics of Circulating Vaccine derived Poliovirus Isolated in the National Reference Polio Laboratory from the Democratic Republic of Congo

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