High-fat, high-carbohydrate diet-induced experimental model of metabolic syndrome in rats

Birulina, Yu. G.; Иванов, В. В.; Buyko, E. E.; Bykov, V. V.; Smagliy, L. V.; Носарев, А. В.; Петрова, Ірина Анатоліївна; Гусакова, С. В.

doi:10.20538/1682-0363-2020-4-14-20

Cited by 13 publications

(5 citation statements)

References 23 publications

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“…At the same time, there is an increase in pathogenic microflora in the gums, the development of inflammation against the background of a decrease in non-specific immunity and the activity of antioxidant protection. Our data is confirmed by the research of scientists [3,8,18].…”

Section: Discussionsupporting

confidence: 89%

“…The rats of group II were simulated MS by introducing 20 % internal lard into the diet, and 10 % fructose solution was used instead of drinking water. The duration of MS simulation was 70 days [7][8][9]. The introduction of these substances into the diet were explained by a possibility of creating disorders of metabolism of carbohydrates and lipids of an alimentary nature in the body of animals typical for MS manifestations.…”

Section: Methodsmentioning

confidence: 99%

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Use of algorithm of the prevention complex of inflammatory processes in the oral cavity in metabolic syndrome

Kravchenko

Appelhans

Poliakov

et al. 2022

SR: PS

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The aim of the work was to evaluate in the experiment the effectiveness of the developed treatment-and-prophylactic complex for the prevention of periodontal tissue disorders under metabolic syndrome simulation. Materials and methods: The study of biochemical and immunological changes in the blood serum, liver and gingival tissue was performed during simulation on the metabolic syndrome of alimentary genesis on Wistar rats, all animals were divided into 5 groups: 1) intact, 2) with simulated metabolic syndrome, 3) in a week after the start of MS simulation 5 times a week in the morning perorally administered a “Capillaroprotect” aqueous solution (bioflavonoid, antioxidant) produced by “Ekosvit Oil” (Ukraine) at a rate of 135 mg/kg, 4) under similar conditions receiving the preparation based on the dihydroquercetin were locally applied on gums a new dental elixir based on bee products and adaptogens of plant origin by o,5 ml/rat with a tampon, which has held for 5–7 minutes, 5) during MS simulation from the second week were used the preparation based on the dihydroquercetin, the dental elixir topically on gums and physiotherapy. Results: Under conditions of experimental MS simulation with a diet rich in saturated fats and carbohydrate there are systemic disorders in the body: reduced nonspecific antimicrobial protection, increased microbial contamination, intensification of lipid peroxidation, the development of inflammation and hepatotoxicity. Prophylactic administration of the proposed dihydroquercetin preparation to animals in the process of simulation of MS significantly inhibits the established disorders, positively affecting the biochemical parameters of the blood serum, liver tissue, periodontium, reducing triglycerides, total cholesterol, glucose level, restoring the state of non-specific resistance, lipid metabolism, preventing inflammation and hepatosis, as well as contamination with pathogenic microflora. Conclusion. The proposed treatment-and-prophylactic complex, which includes the dihydroquercetin preparation, used per os in combination with local therapy of periodontal tissues with a tooth elixir based on propolis and biologically active substances of plant origin adaptogens with ultraphonophoresis under induced metabolic syndrome significantly removed the negative effects of its most important components

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Use of algorithm of the prevention complex of inflammatory processes in the oral cavity in metabolic syndrome

Kravchenko

Appelhans

Poliakov

et al. 2022

SR: PS

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“…To get diet-induced MetS animal models, different types of diets are used such as high-carbohydrate diets, high-fat diets, and high-fat high-carbohydrate diets [ 33 , 116 , 117 ]. A recent analysis [ 8 ] indicated that high carbohydrate diets сan be recommended for studying the early stages of MetS, before diabetes 2 type onset, whereas high-fat diets and high-fat high-carbohydrate diets induce the severe features of MetS faster.…”

Section: Limitations and Shortcomings Of The Animal Modelsmentioning

confidence: 99%

Metabolic Syndrome: Lessons from Rodent and Drosophila Models

Vatashchuk

Bayliak

Hurza

et al. 2022

BioMed Research International

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Overweight and obesity are health conditions tightly related to a number of metabolic complications collectively called “metabolic syndrome” (MetS). Clinical diagnosis of MetS includes the presence of the increased waist circumference or so-called abdominal obesity, reduced high density lipoprotein level, elevated blood pressure, and increased blood glucose and triacylglyceride levels. Different approaches, including diet-induced and genetically induced animal models, have been developed to study MetS pathogenesis and underlying mechanisms. Studies of metabolic disturbances in the fruit fly Drosophila and mammalian models along with humans have demonstrated that fruit flies and small mammalian models like rats and mice have many similarities with humans in basic metabolic functions and share many molecular mechanisms which regulate these metabolic processes. In this paper, we describe diet-induced, chemically and genetically induced animal models of the MetS. The advantages and limitations of rodent and Drosophila models of MetS and obesity are also analyzed.

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“…Диета с высоким содержанием углеводов и высоким содержанием жиров (ВУВЖД, 90 дней) включала 16% белков, 21% жиров, 46% углеводов, в том числе 17% фруктозы, 0,125% холестерина [12]. Вода была заменена 20%-м раствором фруктозы.…”

Section: материал и методыunclassified

Age-related features of developing insulin resistance and adipocyte sensitivity to insulin in rats with induced metabolic syndrome

Нарыжная

Бирулина

Kurbatov

et al. 2021

SJCEM

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Old age and insulin resistance accompanying metabolic syndrome are significant factors in the development of cardiovascular pathology.Aim. The aim of the study was to identify the age-related characteristics of developing insulin resistance on the body level and insulin resistance in rat adipocytes in animals with induced metabolic syndrome.Material and Methods. The study was carried out on male Wistar rats, which were divided into the following groups: group 1 (n = 14) comprised intact rats aged 150 days at the end of study; group 2 (n = 14) comprised rats aged 150 days at the end of 90-day period on diet with high contents of carbohydrates and fats (HCHFD); group 3 (n = 14) comprised intact rats aged 540 days at the end of the study; group 4 (n = 14) comprised rats aged 540 days after the end of 90-day HCHFD. Diet composition was as follows: 16% proteins, 21% fats, 46% carbohydrates including 17% fructose, 0.125% cholesterol, and replacement of drinking water with a 20%-fructose solution. At the end of the diet, body and organ weights were measured, and contents of glucose, insulin, triglycerides, serum leptin, and liver triglycerides were assessed. Epididymal adipose tissue adipocytes were isolated enzymatically. The content of reactive oxygen species (ROS) was investigated using 2,3-dihydrodichlorofluorescein diacetate. Increased ROS production and lipolysis inhibition in response to insulin were observed.Results. The signs of metabolic syndrome were observed in both age groups of HCHFD animals and included visceral obesity, hyperglycemia, and insulin resistance measured by homeostatic model assessment of insulin resistance (HOMA-IR). However, the obesity indicators were more pronounced in the group of young rats, whereas the signs of insulin resistance prevailed in older rats. Leptinemia directly correlated with HOMA-IR (rSp = 0.485, p = 0.03). Insulin resistance of adipocytes was observed in rats older than 540 days after the HCHFD.Conclusion. The obtained results suggested that age was a risk factor for the development of insulin resistance on the body level. Aging resulted in a decrease in the adipocyte sensitivity to insulin in metabolic syndrome. An increase in leptin may be a possible mechanism for worsening of insulin resistance with age.

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High-fat, high-carbohydrate diet-induced experimental model of metabolic syndrome in rats

Cited by 13 publications

References 23 publications

Use of algorithm of the prevention complex of inflammatory processes in the oral cavity in metabolic syndrome

Use of algorithm of the prevention complex of inflammatory processes in the oral cavity in metabolic syndrome

Metabolic Syndrome: Lessons from Rodent and Drosophila Models

Age-related features of developing insulin resistance and adipocyte sensitivity to insulin in rats with induced metabolic syndrome

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