High-fat diet reduces local myostatin-1 paralog expression and alters skeletal muscle lipid content in rainbow trout, Oncorhynchus mykiss

Galt, Nicholas J.; Froehlich, Jacob Michael; Meyer, Ben M.; Barrows, Frederic T.; Biga, Peggy R.

doi:10.1007/s10695-013-9893-4

Cited by 15 publications

(14 citation statements)

References 62 publications

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“…Further, trends were observed in both myostatin-1a and -1b expression in response to the vehicle in both tissues, though not statistically significant. These data, along with our previous work showing changes in myostatin expression in response to a high-fat diet, provide evidence that myostatin may be responsive to lipid availability (Galt et al, 2013). …”

Section: Discussionsupporting

confidence: 77%

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The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss

Galt

Froehlich

Remily

et al. 2014

Comparative Biochemistry and Physiology Part A: Molecular &

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Glucocorticoids (GCs) strongly regulate myostatin transcript levels in mammals via glucocorticoid response elements (GREs) in the myostatin promoter, and bioinformatics methods suggest that this regulatory mechanism is conserved among many vertebrates. However, the multiple myostatin genes found in some fishes may be an exception. In rainbow trout (Oncorhynchus mykiss), two genome duplication events have produced three putatively functional myostatin genes, myostatin-1a, -1b and -2a, which are ubiquitously and differentially expressed. In addition, in silico promoter analyses of the rainbow trout myostatin promoters have failed to identify putative GREs, suggesting a divergence in myostatin function. Therefore, we hypothesized that myostatin mRNA expression is not regulated by glucocorticoids in rainbow trout. In this study, both juvenile rainbow trout and primary trout myoblasts were treated with cortisol to examine the relationship between this glucocorticoid and myostatin mRNA expression. Results suggest that exogenous cortisol does not regulate myostatin-1a and -1b expression in vivo, as myostatin mRNA levels were not significantly affected by cortisol treatment in either red or white muscle tissue. In red muscle, myostatin-2a levels were significantly elevated in the cortisol treatment group relative to the control, but not the vehicle control, at both 12 h and 24 h post-injection. As such, it is unclear if cortisol was acting alone or in combination with the vehicle. Cortisol increased myostatin-1b expression in a dose-dependent manner in vitro. Further work is needed to determine if this response is the direct result of cortisol acting on the myostatin-1b promoter or through an alternative mechanism. These results suggest that regulation of myostatin by cortisol may not be as highly conserved as previously thought and support previous work that describes potential functional divergence of the multiple myostatin genes in fishes.

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Section: Discussionsupporting

confidence: 77%

“…Samples were then centrifuged and plasma was isolated from packed cells. Glucose levels were analyzed in plasma (5 μL) using an Accu-Chek glucose meter (Roche) as previously described (Galt et al, 2013; Meyer et al, 2013). …”

Section: Methodsmentioning

confidence: 99%

The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss

Galt

Froehlich

Remily

et al. 2014

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Several studies have shown that diet may affect the gene MSTN 1. In their study on Oncorhynchus mykiss (rainbow trout) with low (10%) and high (25%) lipid levels, Galt et al (2014) reported a significant decrease in gene expression in animals fed at high lipid rates. The authors underscored a possible relationship between lipid deposition and energy partition with a decrease in myostatin.…”

Section: Discussionmentioning

confidence: 99%

Effect of propolis ethanol extract on myostatin gene expression and muscle morphometry of Nile tilapia in net cages

Buck¹,

Mizubuti

Alfieri

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but its effects on morphometry and muscle gene expression are largely unknown. The present study evaluates the influence of propolis on muscle morphometry and myostatin gene expression in Nile tilapia (Oreochromis niloticus) bred in net cages. Reversed males (GIFT strain) with an initial weight of 170 ± 25 g were distributed in a (2 x 4) factorial 2 E.L. Buck et al.Genetics and Molecular Research 16 (1): gmr16019404 scheme, with two diets (DPRO, commercial diet with 4% propolis ethanol extract and DCON, commercial diet without propolis, control) and four assessment periods (0, 35, 70, and 105 experimental days). Muscles were evaluated at each assessment period. Histomorphometric analysis classified the fiber diameters into four groups: <20 mm; 20-30 mm; 30-50 mm; and > 50 mm. RT-qPCR was performed to assess myostatin gene expression. Fibers < 20 µm diameter were more frequent in DPRO than in DCON at all times. Fiber percentages >30 µm (30-50 and > 50 µm) at 70 days were 25.39% and 40.07% for DPRO and DCON, respectively. There was greater myostatin gene expression at 105 days, averaging 1.93 and 1.89 for DCON and DPRO, respectively, with no significant difference in any of the analyzed periods. Propolis ethanol extract did not affect the diameter of muscle fibers or the gene expression of myostatin. Future studies should describe the mechanisms of natural products' effects on muscle growth and development since these factors are highly relevant for fish production performance.

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“…Diets with low and high fat were tested to determine MSTN expression in the rainbow trout fish (Oncorhynchus mykiss) by Galt et al (2014). They observed that a highfat diet reduces MSTN expression and alters skeletal muscle lipid content.…”

Section: Discussionmentioning

confidence: 99%

Research Article Soybean oil added to the diet reduces Myostatin gene expression in Longissimus dorsi muscle of sheep

Souza¹,

Rêgo²,

Faturi³

et al. 2019

Genet. Mol. Res.

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Myostatin is a protein involved in the regulation of myogenesis; animal meat quality can be influenced by its expression. Animals with low myostatin levels have increased muscle mass and are relatively stronger. We analyzed the influence of the addition of soybean, used soybean and palm oils to the diet on Myostatin gene expression in the Logissimus dorsi muscle of sheep reared in the Northeast Amazon region. A basic control diet was elaborated and used with the addition of 4% of the different oils. All animals were slaughtered at a weight of 35 kg and 5 g of Logissumus dorsi muscle was collected and RNA extracted, quantified and a RT-PCR was run. The control diet, without added oil, gave the highest Myostatin expression levels among all treatments. When unused soybean oil was added to the diet, it significantly decreased Myostatin expression and induced muscle hyperplasia, generating animals with greater musculature. The other oils did not significantly affect expresson of this gene.

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High-fat diet reduces local myostatin-1 paralog expression and alters skeletal muscle lipid content in rainbow trout, Oncorhynchus mykiss

Cited by 15 publications

References 62 publications

The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss

The effects of exogenous cortisol on myostatin transcription in rainbow trout, Oncorhynchus mykiss

Effect of propolis ethanol extract on myostatin gene expression and muscle morphometry of Nile tilapia in net cages

Research Article Soybean oil added to the diet reduces Myostatin gene expression in Longissimus dorsi muscle of sheep

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