High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients

Støen, Maria Jenvin; Andersen, Sigve; Rakaee, Mehrdad; Pedersen, Mona Irene; Ingebriktsen, Lise M.; Bremnes, Roy M.; Dønnem, Tom; Lombardi, Ana Paola G.; Kilvaer, Thomas K.; Busund, Lill-Tove; Richardsen, Elin

doi:10.1038/s41598-021-93208-6

Cited by 29 publications

(22 citation statements)

References 51 publications

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“…In addition, high expression of hsa-miR-183-5p in local tissues of advanced prostate cancer may be related to lymph node metastasis and diffusion (25). Collectively, the three core miRNAs in this study were abnormally highly expressed in prostate cancer tissues, which was consistent with previous studies (16,21,23).…”

Section: Discussionsupporting

confidence: 90%

“…This process participates in the post-transcriptional regulation of cancer-related genes (22). In addition, high expression of hsa-miR-17-5p in the epithelium was a predictive marker for poor prognosis in patients with PCa (23). Furthermore, circRNA-ITCH inhibited PCa progression by sponging hsa-miR-17-5p and increasing HOXB13 expression (24).…”

Section: Discussionmentioning

confidence: 99%

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Construction of miRNA-mRNA network and a nomogram model of prognostic analysis for prostate cancer

Su¹,

Dai²,

Zhang³

2022

Transl Cancer Res TCR

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Background: Dysregulated genetic factors correlate with carcinoma progression. However, the hub miRNAs-mRNAs related to biochemical recurrence in prostate cancer remain unclear. We aim to identify potential miRNA-mRNA regulatory network and hub genes in prostate cancer.Methods: Datasets of gene expression microarray were downloaded from Gene Expression Omnibus (GEO) database for Robust Rank Aggregation (RRA), targeted gene prediction, gene function and signal pathway enrichment analyses, miRNA-mRNA regulatory network construction, core network screening, as well as validation and survival analysis were carried out by using exogenous data.Results: Prostate cancer-related differentially expressed genes were mostly related to actin filament regulation. Moreover, the cGMP-PKG signaling pathway might play a role in prostate cancer progression.As the core of microRNAs, hsa-miR-106b-5p, hsa-miR-17-5p and hsa-miR-183-5p were matched to hub genes (such as TMEM100, FRMD6, NBL1 and STARD4). The expression levels of hub genes in prostate cancer tissues were significantly lower than normal and closely related to prognosis of patients. The ridge regression model was applied to establish a risk score system. Both risk score and Gleason were used to establish a nomogram. Nomogram predicted the area under the [receiver operating characteristic (ROC)] curve (AUC) of biochemical recurrence at 1-, 3-, and 5-year of 0.713, 0.732 and 0.753, respectively.Conclusions: Hub genes were closely related to prostate cancer development and progression, which might become biomarkers for diagnosis and prognosis. This novel nomogram established could be applied to clinical prediction.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Construction of miRNA-mRNA network and a nomogram model of prognostic analysis for prostate cancer

Su¹,

Dai²,

Zhang³

2022

Transl Cancer Res TCR

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“…The cell lines were tested for viability, migration and invasion in the absence and presence of miR-17-5p and miR-20a-5p as previously described by Stoen et al . 45 , 46 . The most important steps of each assay are referred below.…”

Section: Methodsmentioning

confidence: 99%

High expression of miR-17-5p and miR-20a-5p predicts favorable disease-specific survival in stage I-III colon cancer

Selven

Andersen

Pedersen

et al. 2022

Sci Rep

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In many types of cancer, microRNAs (miRs) are aberrantly expressed. The aim of this study was to explore the prognostic impact of miR-17-5p and miR-20a-5p in colon cancer. Tumor tissue from 452 stage I-III colon cancer patients was retrospectively collected and tissue microarrays constructed. miR-17-5p and miR-20a-5p expression was evaluated by in situ hybridization and analyzed using digital pathology. Cell line experiments, using HT-29 and CACO-2, were performed to assess the effect of miR-17-5p and miR-20a-5p over expression on viability, invasion and migration. In multivariate analyses, high miR-17-5p expression in tumor (HR = 0.43, CI 0.26–0.71, p < 0.001) and high expression of miR-20a-5p in tumor (HR = 0.60, CI 0.37–0.97, p = 0.037) and stroma (HR = 0.63, CI 0.42–0.95, p = 0.027) remained independent predictors of improved disease-specific survival. In cell lines, over expression of both miRs resulted in mitigated migration without any significant effect on viability or invasion. In conclusion, in stage I-III colon cancer, high expression of both miR-17-5p and miR-20a-5p are independent predictors of favorable prognosis.

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“…Importantly, no prostate-specific miRNAs have yet been definitively identified. We previously studied the association between several miRNAs and prostate cancer recurrence and survival [ 23 , 24 , 25 , 26 , 27 , 28 ]. High expressions of miR-205, miR-17-5p, miR-20a-5p, miR-210, and miR-141 and a low expression of miR-424 were all associated with an increased risk of prostate cancer recurrence.…”

Section: Introductionmentioning

confidence: 99%

Expression of miR-24-1-5p in Tumor Tissue Influences Prostate Cancer Recurrence: The PROCA-life Study

Stikbakke

Wilsgaard

Haugnes

et al. 2022

Cancers

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The role of miR-24-1-5p and its prognostic implications associated with prostate cancer are mainly unknown. In a population-based cohort, the Prostate Cancer Study throughout life (PROCA-life), all men had a general health examination at study entry and were followed between 1994 and 2016. Patients with available tissue samples after a prostatectomy with curative intent were identified (n = 189). The tissue expression of miR-24-1-5p in prostate cancer was examined by in situ hybridization (ISH) in tissue microarray (TMA) blocks by semi-quantitative scoring by two independent investigators. Multivariable Cox regression models were used to study the associations between miR-24-1-5p expression and prostate cancer recurrence. The prostate cancer patients had a median age of 65.0 years (range 47–75 years). The Cancer of the Prostate Risk Assessment Postsurgical Score, International Society of Urological Pathology grade group, and European Association of Urology Risk group were all significant prognostic factors for five-year recurrence-free survival (p < 0.001). Prostate cancer patients with a high miR-24-1-5p expression (≥1.57) in the tissue had a doubled risk of recurrence compared to patients with low expression (HR 1.99, 95% CI 1.13–3.51). Our study suggests that a high expression of miR-24-1-5p is associated with an increased risk of recurrence of prostate cancer after radical prostatectomy, which points to the potential diagnostic and therapeutic value of detecting miR-24-1-5p in prostate cancer cases.

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High expression of miR-17-5p in tumor epithelium is a predictor for poor prognosis for prostate cancer patients

Cited by 29 publications

References 51 publications

Construction of miRNA-mRNA network and a nomogram model of prognostic analysis for prostate cancer

Construction of miRNA-mRNA network and a nomogram model of prognostic analysis for prostate cancer

High expression of miR-17-5p and miR-20a-5p predicts favorable disease-specific survival in stage I-III colon cancer

Expression of miR-24-1-5p in Tumor Tissue Influences Prostate Cancer Recurrence: The PROCA-life Study

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