Expression of miR-24-1-5p in Tumor Tissue Influences Prostate Cancer Recurrence: The PROCA-life Study

Stikbakke, Einar; Wilsgaard, Tom; Haugnes, Hege Sagstuen; Pedersen, Mona Irene; Knutsen, Tore; Støyten, Martin; Giovannucci, Edward; Eggen, Anne Elise; Thune, Inger; Richardsen, Elin

doi:10.3390/cancers14051142

Cited by 5 publications

(4 citation statements)

References 54 publications

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“…Such results were independently validated in yet another study with RP specimens from 475 patients 34 . Moreover, levels of several other miRNAs assessed in RP samples (in tumour and/or adjacent tissue) also associated with BCR, some even after adjusting for classical clinicopathological variables 35–46 …”

Section: Prostate Cancermentioning

confidence: 64%

“…34 Moreover, levels of several other miRNAs assessed in RP samples (in tumour and/or adjacent tissue) also associated with BCR, some even after adjusting for classical clinicopathological variables. [35][36][37][38][39][40][41][42][43][44][45][46] Serum miR-146b-3p was a significant predictor of time to BCR, independent of clinicopathological parameters. 47 in patients treated by RP.…”

Section: Can Mirnas Predict Biochemical Recurrence/clinical Recurrenc...mentioning

confidence: 91%

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MiRNA biomarkers in cancers of the male reproductive system: Are we approaching clinical application?

et al. 2022

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Background: Specific cancer types face specific clinical management challenges.Owing to their stability, robustness and fast, easy and cost-effective detection, microRNAs (miRNAs) are attractive candidate biomarkers to the clinic.Objectives: Based on a comprehensive review of the relevant literature in the field, we explore the potential of miRNAs as biomarkers to answer relevant clinical dilemmas inherent to cancers of the male reproductive tract (prostate [PCa], testis [TGCTs] and penis [PeCa]) and identify some of the challenges/limitations hampering their widely application. Results and discussion:We conclude that the use of miRNAs as biomarkers is at different stages for these distinct cancer types. While for TGCTs, miRNA-371a-3p is universally accepted to fill in important clinicals gaps and is moving fast towards clinical implementation, for PCa almost no overlap of miRNAs exists between studies, denoting the absence of a consistent miRNA biomarker, and for PeCa the field of miRNAs has just recently started, with only a few studies attempting to explore their clinical usefulness. Conclusion:Technological advances influencing miRNA detection and quantification will be instrumental to continue to move forward with implementation of miRNAs in the clinic as biomarkers for non-invasive diagnosis, risk stratification, treatment monitoring and follow-up.

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Section: Prostate Cancermentioning

confidence: 64%

Section: Can Mirnas Predict Biochemical Recurrence/clinical Recurrenc...mentioning

confidence: 91%

MiRNA biomarkers in cancers of the male reproductive system: Are we approaching clinical application?

et al. 2022

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“…This list of 92 cnvd -miRNAs was an assortment of novel predictive candidates and acknowledged markers of radiation and cancer. For instance, miR-24-1-5p 64 , miR-30e-3p 65 , miR-28-5p 66 , miR-142-3p 67 , miR-148a-5p 68 , miR-455-3p 69 , miR-139-3p 70 , miR-1306-5p 71 and several others mentioned in Table 1 were linked to various radiation and carcinogenesis studies. On the other hand, there are some candidates in the list of 92 cnvd -miRNAs, such as miR-30a-3p and miR-199b-5p, which had not previously linked to minipig radiation model.…”

Section: Discussionmentioning

confidence: 99%

Early to sustained impacts of lethal radiation on circulating miRNAs in a minipig model

Chakraborty,

Holmes-Hampton,

Gautam

et al. 2023

Sci Rep

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Early diagnosis of lethal radiation is imperative since its intervention time windows are considerably short. Hence, ideal diagnostic candidates of radiation should be easily accessible, enable to inform about the stress history and objectively triage subjects in a time-efficient manner. Therefore, the small molecules such as metabolites and microRNAs (miRNAs) from plasma are legitimate biomarker candidate for lethal radiation. Our objectives were to comprehend the radiation-driven molecular pathogenesis and thereby determine biomarkers of translational potential. We investigated an established minipig model of LD70/45 total body irradiation (TBI). In this pilot study, plasma was collected pre-TBI and at multiple time points post-TBI. The majority of differentially expressed miRNAs and metabolites were perturbed immediately after TBI that potentially underlined the severity of its acute impact. The integrative network analysis of miRNA and metabolites showed a cohesive response; the early and consistent perturbations of networks were linked to cancer and the shift in musculoskeletal atrophy synchronized with the comorbidity-networks associated with inflammation and bioenergy synthesis. Subsequent comparative pipeline delivered 92 miRNAs, which demonstrated sequential homology between human and minipig, and potentially similar responses to lethal radiation across these two species. This panel promised to retrospectively inform the time since the radiation occurred; thereby could facilitate knowledge-driven interventions.

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“…However, the functional research on miR-24-1-5p is still in the exploratory stage. Stikbakke et al found that high expression of miR-24-1-5p is associated with an increased risk of prostate cancer recurrence after radical prostatectomy, indicating its potential diagnostic and therapeutic value in prostate cancer cases [40]. Overexpression of miR-24-1-5p in human colorectal cancer cells signi cantly inhibits β-catenin expression and reduces cell proliferation, migration, and survival [41].…”

Section: Disscussionmentioning

confidence: 99%

Anti-inflammatory effects of Placenta-derived exosomal miR-24-1-5p through targeting TNFAIP8 and its implication in enhancing placental endotoxin tolerance in a model of lipopolysaccharide (LPS)-induced placental inflammation

Zhang

Chen

et al. 2023

Preprint

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Objective and design: Intra-amniotic infection (IAI) represents a potentially catastrophic complication during parturition, with potential for significant maternal and neonatal consequences. As a crucial immunological organ at the maternal-fetal interface, the immunoregulatory capacities of the placenta and the underlying molecular dynamics at play in the pathogenesis of IAI continue to be unclear. Recent studies have found that placenta-derived exosomes (Pd-Exos) and their contained microRNAs (miRNAs) may be involved in immune regulation during pregnancy. This study aimed to investigate the immunoregulatory function of the placenta in connection with the progression of IAI, as well as to elucidate its potential molecular mechanisms. Material or subjects: An IAI model was developed by co-culturing full-term placental explants obtained from elective cesarean sections with lipopolysaccharide (LPS). Using RT-qPCR and ELISA, the dynamic expression patterns of inflammatory factors within the placental explants were identified at varying time points post-LPS exposure. Pd-Exos were then isolated from the culture supernatant of placental explants and subjected to miRNA sequencing to pinpoint exosomal miRNAs integral to the immunoregulatory functions of the placenta. Subsequently, the role of a differentially expressed exosomal miRNA was validated. Results In the early phase of LPS stimulation, extensive pro-inflammatory responses were observed in placental explants, evidenced by the augmented expression of TNF-α and IL-1β. However, as the LPS stimulation progressed to the latter stages, a decrease in the pro-inflammatory response was noted, alongside a gradually surging anti-inflammatory response, signified by a diminishing ratio between pro-inflammatory and anti-inflammatory indicators (TNF-α/IL-10 and IL-1β/IL-1Ra). Following prolonged LPS stimulation of placental explants, the expression of miR-24-1-5p was upregulated in Pd-Exos. Upon internalization by receptor cells (THP-1 cells and Swan 71 cells), miR-24-1-5p can inhibit the expression of its target gene Tumor necrosis factor alpha-induced protein 8 (TNFAIP8), thereby suppressing the expression of downstream inflammatory factors TNF-α and IL-1β. Conclusion Prolonged exposure to the LPS in human term placental tissues induced endotoxin tolerance. Additionally, the placenta-derived exosomal miR-24-1-5p down-regulated the expression of the inflammatory markers TNF-α and IL-1β by inhibiting the functionality of TNFAIP8, thereby contributing to the placental endotoxin tolerance.

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Expression of miR-24-1-5p in Tumor Tissue Influences Prostate Cancer Recurrence: The PROCA-life Study

Cited by 5 publications

References 54 publications

MiRNA biomarkers in cancers of the male reproductive system: Are we approaching clinical application?

MiRNA biomarkers in cancers of the male reproductive system: Are we approaching clinical application?

Early to sustained impacts of lethal radiation on circulating miRNAs in a minipig model

Anti-inflammatory effects of Placenta-derived exosomal miR-24-1-5p through targeting TNFAIP8 and its implication in enhancing placental endotoxin tolerance in a model of lipopolysaccharide (LPS)-induced placental inflammation

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