High-efficient extraction of principal medicinal components from fresh Phellodendron bark (cortex phellodendri)

Xu, Keqin; He, Gongxiu; Qin, Jieming; Cheng, Xuexiang; He, Hanjie; Zhang, Dangquan; Peng, Wanxi

doi:10.1016/j.sjbs.2017.10.008

Cited by 33 publications

(26 citation statements)

References 25 publications

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“…When leaves of O. Fragrance were under high temperature stress, ADH gene expression would be increased because of the resistance response mechanism activation in order to avoid from heat damage. Many researches about functional components in the leaves, flowers (Ge et al, 2018a and2018b), barks (Ge et al, 2017a and, stems (Ge et al, 2017c and2017d), roots (Ge et al, 2017e;, fruits (Jiang et al, 2018a and2018b), timbers (Peng et al, 2017a and and byproducts (Liu et al, 2017;Xie et al, 2018) of plants showed that they contain many bioactive components (Peng et al, 2016;He et al, 2017;Cheng et al, 2017;Yang et al, 2017;Qin et al, 2017;Xu et al, 2018;Wang et al, 2018). This result was consistent with the resistance response function of ADH (Strommer et al, 2011).…”

Section: Discussionsupporting

confidence: 70%

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Zhang

2018

Emir J Food Agric

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Because of a lot of aroma components in the flowers, Osmanthus fragrans is widely used as ornamental tree species and its flowers is used as materials of spices and food additives. Alcohol dehydrogenase (ADH) plays an important regulation role in growth, development, stress resistance and aroma synthesis of plants. Using the flowers of O fragrans var. semperflorens as materials, the full-length cDNA of ADH gene (termed OfADH) was successfully cloned through rapid amplification of cDNA ends (RACE), which has the 1128 bp open reading frame (ORF) in length and codes 375 amino acids. The results of conserved domain analysis show that the OfADH belongs to the zinc-dependent medium-chain dehydrogenase /reductase (MDR) superfamily, contains 22 NAD(P) binding sites in the liver_alcohol_DH_like conserved domains, and also has the PLN02740 (Alcohol dehydrogenase-like) domain. The results of semi-quantitative RT-PCR indicate that the expression level of OfADH in O. fragrans flowers is consistent with the rule of aroma formation and release, while the expression of OfADH in leaves is positively correlated with high temperature stress, suggesting that OfADH has obvious functional diversity in regulation of aroma synthesis and response to high temperature stress.

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Section: Discussionsupporting

confidence: 70%

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Zhang

2018

Emir J Food Agric

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“…FA was applied to quantify alkaloids in different parts of eight Berberis species [11] while TFA was added into both double‐distilled water and acetonitrile (ACN) for determination of palmatine from barks of Phellodendron amurense Rupr. or P. chinense Schneid [12]. Mobile phase system containing FA, methanol, and water was employed in the analysis of amaryllidaceae alkaloids from herbs of Narcissus tazetta L. [13].…”

Section: Chromatographic Separation Methodsmentioning

confidence: 99%

“…SDS was also employed to determine phellodendrine and berberine from barks of Phellodendron amurense Rupr. or P. chinense Schneid [12]. Quality assessment of isoquinoline alkaloids in herbs of Corydalis impatiens (Pall.)…”

Section: Chromatographic Separation Methodsmentioning

confidence: 99%

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

Zhao

Chen

Yu-qian

et al. 2020

J of Separation Science

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Alkaloids are a widespread group of basic compounds in herbal medicines and have attracted great interest due to various pharmaceutical activities and desirable druggability. Their distinctive structures make chromatographic separation fairly difficult. Peak tailing, poor resolution, and inferior column‐to‐column reproducibility are common obstacles to overcome. In order to provide a valuable reference, the methodologies and/or strategies on liquid chromatographic separation of alkaloids in herbal medicines proposed from 2012 to 2019 are thoroughly summarized.

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“…Some bioactive components were found in benzene extracts which can be used in spices and food additives. Hexadecanoic acid, ethyl ester for perfume flavor, Octadecanoic acid, ethyl ester are mainly used for the preparation of bacon flavor [32][33][34]. Tetradecanoic acid is the raw material of spices.…”

Section: Comparison Of Volatile Components In Two Extractsmentioning

confidence: 99%

Bioenergy and bioactive components in leaves of Toona sinensis

2020

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Toona sinensis is an important tree for urban greening construction. Toona sinensis sprouts are widely used because of their high medicinal and edible value. However, the functions and effective components of mature Toona sinensis leaves have not been fully exploited and utilized. In order to explore the value of Toona sinensis leaves in bioactive and bioenergy utilization, Toona sinensis leaves were extracted with benzene and ethanol, and then the components in extracts were analyzed by FT-IR and GC-MS. The results showed that benzene and ethanol extracts from Toona sinensis leaves mainly contained phenols, hydrocarbons, esters, alcohols, acids and other bioactive components. These bioactive components can be used in the fields of bioenergy, biomedicine, chemical row materials, food additives and spices. Among them, the content of bioactive components from benzene extract was higher than that from ethanol extract. These findings indicate that Toona sinensis leaves contain a large number of bioactive components which can be used in bioenergy and other fields, and have broad prospects for development.

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High-efficient extraction of principal medicinal components from fresh Phellodendron bark (cortex phellodendri)

Abstract: The hydrochloric acid/methanol-ultrasonic extraction has the best effect for three medicinal components of fresh bark, providing an extraction yield of 103.12 mg/g berberine, 24.41 mg/g phellodendrine, 1.25 mg/g palmatine.

Cited by 33 publications

References 25 publications

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

Bioenergy and bioactive components in leaves of Toona sinensis

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High-efficient extraction of principal medicinal components from fresh Phellodendron bark (cortex phellodendri)

Abstract: The hydrochloric acid/methanol-ultrasonic extraction has the best effect for three medicinal components of fresh bark, providing an extraction yield of 103.12 mg/g berberine, 24.41 mg/g phellodendrine, 1.25 mg/g palmatine.

Cited by 33 publications

References 25 publications

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Gene Cloning and Gene Expression Characteristics of Alcohol Dehydrogenase in Osmanthus fragrans var. semperflorens

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

Bioenergy and bioactive components in leaves of Toona sinensis

Contact Info

Product

Resources

About

Abstract: The hydrochloric acid/methanol-ultrasonic extraction has the best effect for three medicinal components of fresh bark, providing an extraction yield of 103.12 mg/g berberine, 24.41 mg/g phellodendrine, 1.25 mg/g palmatine.