“…HPLC determination of curcumin was performed following modification of a method described earlier (Zhan, Zeng, Zhang, & Li, 2011). An Agilent 1100 HPLC system (Agilent, America) with Photo Diode Array (PDA) detector, Agilent TC-C18 column (250 mm  4.6 mm, 5 lm) and analytical software were used for the analysis of curcumin.…”
Section: Determination Of Curcumin Contentmentioning
“…HPLC determination of curcumin was performed following modification of a method described earlier (Zhan, Zeng, Zhang, & Li, 2011). An Agilent 1100 HPLC system (Agilent, America) with Photo Diode Array (PDA) detector, Agilent TC-C18 column (250 mm  4.6 mm, 5 lm) and analytical software were used for the analysis of curcumin.…”
Section: Determination Of Curcumin Contentmentioning
“…CCE was performed in glass chromatographic columns [21][22][23]. For non-cyclic CCE, 8 g tobacco material was loaded into a column with one MV solvent at a bed height to diameter ratio of 10:1 or 5:1, through the wet column preparation method.…”
“…The CCE method includes two steps, dissolving and eluting the target compounds from the plant material in chromatographic columns with minimum volume solvent at room temperature [21][22][23]. As shown in Fig.…”
Section: Extraction Solvent and Conditionsmentioning
confidence: 99%
“…We have reported a highly efficient column-chromatographic extraction (CCE) method for the extraction of natural compounds from biological materials [21][22][23]. In this work, we report a unique procedure for simultaneously extracting nicotine and solanesol and hydrolyzing bound forms or ester forms of nicotine and solanesol using a minimum volume of the optimal solvent, followed by automatic separation and simple purification to economically produce highly-purified nicotine and solanesol using the waste tobacco materials.…”
“…A highly efficient column chromatographic extraction of curcumin from Curcuma longa was proposed by Zhan et al, 2011. Curcumin (Figure 11) was extracted with minimum use of solvent, minimum volume and high concentration of extraction solution by CC.…”
Section: Chromatography and Its Applications 148mentioning
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