Absorption mechanism of whey-protein-delivered curcumin using Caco-2 cell monolayers

Li, Ming; Cui, Jie; Ngadi, Michael; Ma, Ying

doi:10.1016/j.foodchem.2015.01.132

Cited by 70 publications

(40 citation statements)

References 31 publications

(33 reference statements)

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“…permeable compounds. The rate of permeation of curcumin across the cells monolayer increased as function of time (data not shown) and the TEER values (not shown) did not change significantly (p > 0.05), suggesting that curcumin permeated the cells monolayer through transcellular pathway (Li et al, 2015a;Sun et al, 2015;Yu and Huang, 2012). Nevertheless, these results do not show if nanoemulsions permeate directly across the Caco-2 cell monolayers (Yu and Huang, 2012).…”

Section: Permeability Assaymentioning

confidence: 87%

“…their monolayers have intercellular tight junctions, microvilli and exhibit brush-border characteristics at the apical side after confluence) (Zeng et al, 2017). Generally, there are two pathways to transport bioactive compounds through the small intestine epithelium which can be modulated to enhance the absorption of bioactive compounds in the gastrointestinal tract: the paracellular and the transcellular pathways (Li et al, 2015a(Li et al, , 2015bWang et al, 2015;Yu and Huang, 2012). In paracellular transport, lipid-based nanosystems below 50 nm may be able to directly diffuse (passive diffusion) through the cell tight junctions (Li et al, 2015b;Wang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the effect of chitosan layer on bioaccessibility and cellular uptake of curcumin nanoemulsions

Silva

Beldikova

Poejo

et al. 2019

Journal of Food Engineering

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Curcumin nanoemulsions stabilized by whey protein isolate were successfully developed using high-pressure homogenization. The effect of a chitosan layer deposition using the layer-by-layer technique on nanoemulsions' stability was evaluated during storage conditions, as well as during gastrointestinal tract passage. Lipids' hydrolysis and curcumin bioaccessibility was assessed using a dynamic gastrointestinal model (simulating the stomach, duodenum, jejunum and ileum) and the cytotoxicity, cellular antioxidant activity and permeability analyses were carried out using Caco-2 cells. Results showed that both nanosystems were stable during one month of storage and at stomach pH conditions, whereas creaming and phase separation occurred at intestine pH conditions. The addition of a chitosan layer increased curcumin bioaccessibility, whereas cellular antioxidant activity studies revealed that nanoemulsions and multilayer nanoemulsions exhibited 9 and 10 times higher antioxidant capacity at the cellular level, respectively, when compared to free curcumin. Permeability assays showed that the use of a chitosan layer significantly increased the apparent permeability coefficient of curcumin through Caco-2 cells by 1.55-folds.

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Section: Permeability Assaymentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Evaluating the effect of chitosan layer on bioaccessibility and cellular uptake of curcumin nanoemulsions

Silva

Beldikova

Poejo

et al. 2019

Journal of Food Engineering

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“…This property is beneficial for CUR for being delivered into the intestinal tract without its release in the stomach. The permeation rate of β-lactoglobulin-CUR complex was higher than that of digested β-lactoglobulin-CUR complex, both the digestion-absorption and direct diffusion route may exist at the same time in the intestinal tract because the β-lactoglobulin was very sensitive to trypsin 101 . Chopra et al 102 have synthesized biodegradable and non-toxic polymer such as poly (lacticco-glycolic) acid (PLGA) encapsulated formulation of CUR (PLGA-CUR-NPs) with photosafe nature.…”

Section: Biological Activities Of Various Curcuminoid Formulationsmentioning

confidence: 98%

Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives – A review

Amalraj¹,

Pius

Gopi

et al. 2017

Journal of Traditional and Complementary Medicine

708

413

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In recent years, several drugs have been developed deriving from traditional products and current drug research is actively investigating the possible therapeutic roles of many Ayruvedic and Traditional Indian medicinal therapies. Among those being investigated is Turmeric. Its most important active ingredient is curcuminoids. Curcuminoids are phenolic compounds commonly used as a spice, pigment and additive also utilized as a therapeutic agent used in several foods. Comprehensive research over the last century has revealed several important functions of curcuminoids. Various preclinical cell culture and animals studies suggest that curcuminoids have extensive biological activity as an antioxidant, neuroprotective, antitumor, anti-inflammatory, anti-acidogenic, radioprotective and arthritis. Different clinical trials also suggest a potential therapeutic role for curcuminoids in numerous chronic diseases such as colon cancer, lung cancer, breast cancer, inflammatory bowel diseases. The aim of this review is to summarize the chemistry, analog, metal complex, formulations of curcuminoids and their biological activities.

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“…Madalena et al (2016) described that β-Lg/riboflavin nanostructures particle size increased (from 232 ± 21 nm to 329 ± 23 nm) after simulated gastric phase. The presence of intact β-Lg structure in the stomach was depicted by Li, Cui, Ngadi, and Ma (2015). These authors performed an in vitro gastrointestinal digestion of β-Lg/curcumin complex in simulated gastric fluid (SGF).…”

Section: Evaluation Of β-Lg Micro-and Nanostructures Responsiveness Amentioning

confidence: 99%

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

Simões

Martins

Pinheiro

et al. 2020

Food Research International

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Lactoglobulin (β-Lg) is known to be capable to bind hydrophilic and hydrophobic bioactive compounds. This research aimed to assess the in vitro performance of β-Lg micro-(diameter ranging from 200 to 300 nm) and nano (diameter < 100 nm) structures associated to hydrophilic and hydrophobic model compounds on Caco-2 cells and under simulated gastrointestinal (GI) conditions. Riboflavin and quercetin were studied as hydrophilic and hydrophobic model compounds, respectively. Cytotoxicity experiment was conducted using in vitro cellular model based on human colon carcinoma Caco-2 cells. Moreover, the digestion process was simulated using the harmonized INFOGEST in vitro digestion model, where samples were taken at each phase of digestion processoral, gastric and intestinal-and characterized in terms of particle size, polydispersity index (PDI), surface charge by dynamic light scattering (DLS); protein hydrolysis degree by 2,4,6-trinitrobenzene sulfonic acid (TNBSA) assay and native polyacrylamide gel electrophoresis; and bioactive compound concentration. Caco-2 cell viability was not affected up to 21 × 10 −3 mg mL −1 of riboflavin and 16 × 10 −3 mg mL −1 quercetin on β-Lg micro-and nanostructures. In the oral phase, β-Lg structures' particle size, PDI and surface charge values were not changed comparing to the initial β-Lg structures (i.e., before being subjected to in vitro GI digestion). During gastric digestion, β-Lg structures were resistant to proteolytic enzymes and to acid environment of the stomachconfirmed by TNBSA and native gel electrophoresis. In vitro digestion results indicated that β-Lg micro-and nanostructures protected both hydrophilic and hydrophobic compounds from gastric conditions and deliver them to target site (i.e., intestinal phase). In addition, β-Lg structures were capable to enhance riboflavin and quercetin bioaccessibility and bioavailability potential compared to bioactive compounds in their free form. This study indicated that β-Lg micro-and nanostructures were capable to enhance hydrophilic and hydrophobic compounds bioavailability potential and they can be used as oral delivery systems.

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Absorption mechanism of whey-protein-delivered curcumin using Caco-2 cell monolayers

Cited by 70 publications

References 31 publications

Evaluating the effect of chitosan layer on bioaccessibility and cellular uptake of curcumin nanoemulsions

Evaluating the effect of chitosan layer on bioaccessibility and cellular uptake of curcumin nanoemulsions

Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives – A review

β-lactoglobulin micro- and nanostructures as bioactive compounds vehicle: In vitro studies

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