High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad)

Dong, Jin-Zhuo; Jia, Sun

doi:10.1007/bf00232331

Cited by 69 publications

(55 citation statements)

References 12 publications

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“…Young explants have been shown to give better results than older explants in most Brassica species (Chakrabarty et al 2002;Sharma et al 2012;Sharma et al 2014). Dong and Jia (1991) have reported that shoot differentiation frequencies of cotyledonary explants from seedlings older than 7 days dropped sharply. A possible explanation is that young cotyledon and hypocotyl explants are physiologically and biochemically more active as they have less rigid cell wall and are easily affected by the environmental factors such as exogenous plant growth regulators.…”

Section: Resultsmentioning

confidence: 99%

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Kumar

Srivastava

2015

Physiol Mol Biol Plants

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Broccoli (Brassica oleracea L. var. italica) is an important, nutritionally rich vegetable crop, but severely affected by environmental stresses, pests and diseases which cause massive yield and quality losses. Genetic manipulation is becoming an important method for broccoli improvement. In the present study, a reproducible and highly efficient protocol for obtaining organogenesis from hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica cv. Solan green head) has been developed. Hypocotyl and cotyledon explants were used from 10 to 12 days old aseptically grown seedlings whereas leaf and petiole explants were excised from 18 to 20 days old green house grown seedlings and surface sterilized. These explants were cultured on shoot induction medium containing different concentration and combination of BAP and NAA. High efficiency shoot regeneration has been achieved in hypocotyl (83.33 %), cotyledon (90.11 %), leaf (62.96 %) and petiole (91.10 %) explants on MS medium supplemented with 3.5 mg/l BAP+ 0.019 mg/l NAA 2.5 mg/l BAP+0.5 mg/l NAA, 4.0 mg/l BAP+0.5 mg/l NAA and 4.5 mg/l BAP+0.019 mg/l NAA respectively. Petiole explants showed maximum shoot regeneration response as compared to other explants. MS medium supplemented with 0.10 mg/l NAA was found best for root regeneration (100 %) from in vitro developed shoots. The regenerated complete plantlets were transferred to the pots containing cocopeat and successfully acclimatized. This optimized regeneration protocol can be efficiently used for genetic transformation in broccoli. This is the first comparative report on multiple shoot induction using four different types of explants viz. hypocotyl, cotyledon, leaf and petiole.

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Section: Resultsmentioning

confidence: 99%

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Kumar

Srivastava

2015

Physiol Mol Biol Plants

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“…Seedling age is reported to be an important factor for obtaining high frequency adventitious shoot regeneration in watermelon (Choi et al, 1994;Compton & Gray, 1994). For watermelon organogenesis, explants were collected from seedlings younger than five-dayold (Choi et al, 1994;Compton, 2000), as young cotyledons are physiologically very active and respond efficiently to exogenous hormones (Dong & Jia, 1991).…”

Section: Resultsmentioning

confidence: 99%

“…The composition of induction media is also important for adventitious bud development; the presence of cytokinin is critical for shoot induction and for differentiation from cotyledonary explants of watermelon (Dong & Jia, 1991). BAP has been reported as highly Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…Dong & Jia (1991) reported an improvement in shoot bud development in watermelon cotyledons, when combining cytokinin and auxin in the induction media, but both Compton & Gray (1993) and Srisvastava et al (1989) detected an inhibition of shoot organogenesis, when NAA or indole-3-acetic acid (IAA) was added to the induction medium. TDZ has been reported to stimulate organogenesis in several crops (Zhang et al, 2001;Fratini & Ruiz, 2002), but this does not seem to be the case for watermelon, as Compton & Gray (1993) also reported a lower number of explants with shoot buds, using culture media supplemented with this growth regulator.…”

Section: Resultsmentioning

confidence: 99%

“…The system is based on three culture steps (Dong & Jia, 1991): a bud induction phase, culturing the explants in medium supplemented with cytokinin (Compton & Gray, 1993); an elongation phase, transferring the shoot buds to medium with a lower concentration of cytokinin (Dong & Jia, 1991); and, a rooting phase, using a culture medium supplemented with auxin (Compton & Gray, 1994;Dabauza et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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In vitro organogenesis in watermelon cotyledons

Krug

Stipp

Martinelli

et al. 2005

Pesq. agropec. bras.

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-The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L -l ) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants. Adventitious shoots were characterized by the development of shoot apical meristem and leaf primordia. The formation of protuberances, that do not develop into adventitious buds, was also observed.Index terms: Citrullus lanatus, histological study, adventitious bud. Organogênese in vitro em cotilédones de melanciaResumo -O objetivo do trabalho foi estudar a organogênese in vitro de C. lanatus, pela indução de gemas adventícias, em segmentos de cotilédones, cultivados em meio de cultura suplementado com citocinina. Os explantes consistiram de segmentos das regiões distal e proximal de cotilédones, coletados de plantas germinadas in vitro com um, três e cinco dias de idade. Os dados obtidos mostram que a organogênese de melancia, in vitro, ocorre com maior eficiência em segmentos da região proximal dos cotilédones, coletados de plântulas com três dias de idade e cultivados em meio de cultura MS, suplementado com a combinação BAP (1 mg L -l ) e água de coco (10%). Pelo estudo histológico, verificou-se que a organogênese ocorre diretamente, sem a formação de calo, na epiderme e subepiderme do explante. As gemas adventícias foram caracterizadas pela presença de meristema apical e primórdios foliares. Observou-se, também, o desenvolvimento de protuberâncias que não se desenvolvem em gemas adventícias.Termos de indexação: Citrullus lanatus, estudo histológico, gema adventícia.

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Cucurbits

Tricoli

Staub

Kabelka

et al. 2008

Compendium of Transgenic Crop Plants

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There are five cultivated species of the genus Cucurbita: C. pepo , C. moschata , C. maxima , C. argyrosperma , and C. ficifolia . Cucurbita is one of the most morphologically variable genera in the plant kingdom. Breeding of Cucurbita has played a key role in the variety and productivity of cultivars available to growers and consumers today. In addition to traditional plant breeding methods of transferring virus‐resistance alleles from wild to cultivated species of Cucurbita , scientists have developed and commercialized genetically engineered virus resistance in C. pepo . The center of origin for Cucumis species is likely Africa for the wild species, but initial sites of domestication for C. melo are likely in the Middle East. On the other hand, C. sativus may have originated in Africa, China, India, or in the Near East with domestication occurring later throughout Europe. Melon and cucumber's improved varieties have thus far been derived through traditional breeding, which has accomplished a wide range of improvements, including yield and disease resistance. Watermelon was thought to have originated in southern Africa. Most cultivated or edible watermelons belong to the species Citrullus lanatus . Over the past century, conventional breeding has tremendously improved earliness, plant architecture, fruit yield, quality, diversity, disease resistance, and shipping quality.

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High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad)

Cited by 69 publications

References 12 publications

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

In vitro organogenesis in watermelon cotyledons

Cucurbits

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