High efficiency gene transfection by electroporation using a radio-frequency electric field

Electroporation-mediated pain-killer gene therapy for mononeuropathic rats

“…14,15 Unlike viral vectors, the introduction of the plasmid DNA into target cells is facilitated by chemical or physical methods. [16][17][18][19] Electroporation is a physical method of introducing macromolecules into cells in vitro by applying a brief electrical pulse that causes transient membrane transit of molecules, providing higher transfer efficiency compared with other nonviral transformation methods. 19,20 For the present study, a novel method was developed for spinal cord-confined gene transfer in vivo.…”

“…[16][17][18][19] Electroporation is a physical method of introducing macromolecules into cells in vitro by applying a brief electrical pulse that causes transient membrane transit of molecules, providing higher transfer efficiency compared with other nonviral transformation methods. 19,20 For the present study, a novel method was developed for spinal cord-confined gene transfer in vivo. The optimal conditions of intrathecal electroporation for the expression of pro-opiomelanocortin (POMC) gene were determined initially by Western blot.…”

Improving electrotransfection efficiency by post-pulse centrifugation

“…4,5 Also, the parameters in electroporation are easier to control and the efficiency in some cases is higher than those by other methods. [6][7][8][9] Moreover, it avoids biological contamination that potentially exists in virus-induced methods. It offers an attractive way for ex vivo gene delivery if the transfection efficiency can be significantly improved.…”

Therapeutic Applications of Electroporation

“…The transfection efficiency decreases with increasing gap between repeating pulses Chang et al (1991). This also suggests that DNA is collected on the cell surface for subsequent push through the electropores.…”

“…Once the cell is permeabilized by a pulse, more DNA enters the cells during the next pulse of lower field strength Sukharev et al (1992). Multiple pulses and AC pulses seem to have better results Chang et al (1991).…”