High efficiency entrapment of superoxide dismutase into mucoadhesive chitosan-coated liposomes

Rengel, Ružica Galović; Barišić, Karmela; Pavelić, Željka; Grubišić, Tihana Žanić; Čepelak, Ivana; Filipović-Grčić, Jelena

doi:10.1016/s0928-0987(02)00030-1

Cited by 90 publications

(32 citation statements)

References 22 publications

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“…In order to determine the effect of preparation method on enzyme activity, a recovery test was performed. The recovered SOD activity was 85.3% of initial SOD activity that is lower than that reported in literature (7). Therefore, it can be concluded that there was little activity loss during the production procedure.…”

Section: Activitycontrasting

confidence: 57%

“…Activity of SOD was determined with a kit based on ability of enzyme to convert superoxide radicals, produced by xanthine/xanthine oxidase, to hydrogen peroxide and to inhibit indicator oxidation and color formation (6,7,14).…”

Section: Enzyme Assay Methodsmentioning

confidence: 99%

“…Treatment using SOD appears to be a promising alternative to conventional therapy. Various studies have attempted to use it in the treatment of oxidative stress-related diseases such as burns (7). These studies were mainly based on systemic application of SOD.…”

Section: Introductionmentioning

confidence: 99%

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Superoxide Dismutase-Loaded Solid Lipid Nanoparticles Prepared by Cold Homogenization Method: Characterization and Permeation Study Through Burned Rat Skin

Karami

Makhmalzadeh

Koochak

et al. 2016

Jundishapur J Nat Pharm Prod

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Background: Superoxide dismutase (SOD), which inhibits lipid peroxidation and scavengers oxygen radicals, is an effective enzyme for treatment of skin ulcer lesion especially due to burns. Superoxide Dismutase is a hydrophilic compound with high molecular weight and low affinity for partitioning into skin. Moreover, another important limitation for its use in medicine is thermal denaturation and inactivation.

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Section: Activitycontrasting

confidence: 57%

Section: Enzyme Assay Methodsmentioning

confidence: 99%

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Superoxide Dismutase-Loaded Solid Lipid Nanoparticles Prepared by Cold Homogenization Method: Characterization and Permeation Study Through Burned Rat Skin

Karami

Makhmalzadeh

Koochak

et al. 2016

Jundishapur J Nat Pharm Prod

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“…Some liposomal drug delivery systems exhibited superior pharmacological properties to those observed with conventional formulation (20). Activity of liposomes as a carrier for drugs depends upon various factors such as charge, rigidity, composition of the liposomal membrane, encapsulation efficiency and release rate (21)(22)(23)(24). Liposomes can be formulated from a variety of lipid and lipid mixtures with different composition (25), they can be modified in particle size, structure, and surface charge to obtain desirable physicochemical properties to suit particular needs (26,27).…”

Section: Introductionmentioning

confidence: 99%

Ciprofloxacin as Ocular Liposomal Hydrogel

Hosny

2010

AAPS PharmSciTech

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Abstract. The purpose of this study was to prepare and characterize an ocular effective prolonged-release liposomal hydrogel formulation containing ciprofloxacin. Reverse-phase evaporation was used for preparation of liposomes consisting of soybean phosphatidylcholine (PC) and cholesterol (CH). The effect of PC/CH molar ratio on the percentage drug encapsulation was investigated. The effect of additives such as stearylamine (SA) or dicetyl phosphate (DP) as positive and negative charge inducers, respectively, were studied. Morphology, mean size, encapsulation efficiency, and in vitro release of ciprofloxacin from liposomes were evaluated. For hydrogel preparation, Carbopol 940 was applied. In vitro transcorneal permeation through excised albino rabbit cornea was also determined. Optimal encapsulation efficiency of 73.04±3.06% was obtained from liposomes formulated with PC/CH at molar ratio of 5:3 and by increasing CH content above this limit, the encapsulation decreased. Positively charged liposomes showed superior entrapment efficiency (82.01±0.52) over the negatively charged and the neutral liposomes. Hydrogel containing liposomes with lipid content PC, CH, and SA in molar ratio 5:3:1, respectively, showed the best release and transcorneal permeation with the percentage permeation of 30.6%. These results suggest that the degree of encapsulation of ciprofloxacin into liposomes and prolonged in vitro release depend on composition of the vesicles. In addition, the polymer hydrogel used in preparation ensure steady and prolonged transcorneal permeation. In conclusion, ciprofloxacin liposomal hydrogel is a suitable delivery system for improving the ocular bioavailability of ciprofloxacin.

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“…It is slowly soluble in the region of GI tract where juices are neutral to weakly alkaline 21 . By the right combination of liposomal and polymer characteristics, it is possible to develop delivery system with specific, prolonged, and controlled release 22 . The objective of this work was to optimize the mucoadhesive liposomal delivery system for oral administration of peptides and possibly phytochemicals by the optimization of coating mucoadhesive material.…”

mentioning

confidence: 99%

Mucoadhesive liposomal delivery systems: the choice of coating material

Karn

Vanić

Pepić

et al. 2010

Drug Development and Industrial Pharmacy

120

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Objective: Development of liposomal mucoadhesive drug delivery system, which is able to improve the bioavailability of poorly absorbed oral drugs by prolonging their gastric and intestinal residence time, through facilitating the intimate contact of the delivery system with the absorption membrane. Materials and methods: Liposomes containing model drug atenolol were prepared by the modified ethanol injection method. Liposomes containing atenolol were coated by different mucoadhesive polymers, for example, chitosan, Carbopol 974P, Eudragit L100, and Eudragit S100, to optimize the choice of coating material. The delivery systems were tested for their in vitro mucoadhesiveness. Results: Liposomes prepared by the ethanol injection method were of satisfactory size (around 100 nm, before coating). Through the coating of liposomes in the presence of unentrapped material, the entrapment efficiency for drug was increased. In vitro mucoadhesive test confirmed the mucoadhesive properties of the coated layer for all tested polymers; however, Eudragit S100-coated liposomes were superior to other coating materials. Discussion: Eudragit coating appeared to be an optimal polymer choice. These preliminary data indicate that polymer-coated mucoadhesive liposomes are able to carry sufficient amount of drug and to remain attached to the intestinal mucosa for a sufficient period of time to enable prolonged absorption of entrapped drug. Conclusion: While keeping in mind that the in vivo conditions may vary with the in vitro ones, we may recommend the system described in our work for possible oral delivery of peptides and phytochemicals.

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High efficiency entrapment of superoxide dismutase into mucoadhesive chitosan-coated liposomes

Cited by 90 publications

References 22 publications

Superoxide Dismutase-Loaded Solid Lipid Nanoparticles Prepared by Cold Homogenization Method: Characterization and Permeation Study Through Burned Rat Skin

Superoxide Dismutase-Loaded Solid Lipid Nanoparticles Prepared by Cold Homogenization Method: Characterization and Permeation Study Through Burned Rat Skin

Ciprofloxacin as Ocular Liposomal Hydrogel

Mucoadhesive liposomal delivery systems: the choice of coating material

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