High doses of alcohol during pregnancy cause DNA damages in osteoblasts of newborns rats

Carvalho, Isabel Chaves Silva; Dutra, Tamires Pereira; Andrade, Dennia Perez de; Balducci, Ivan; Soares, Cristina Pacheco; Rocha, Rosilene Fernandes da

doi:10.1002/bdra.23468

Cited by 12 publications

(13 citation statements)

References 66 publications

(130 reference statements)

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“…The Comet Assay technique was originally developed by [53] and modified for human blood cells by [37]. It is considered a relatively simple, rapid and sensitive technique to detect various sorts of DNA damage, including oxidative damage inflicted by ROS at single-cell level at a reasonably low cost [36,[54][55][56][57][58]. This powerful technique facilitates the denaturation, unwinding, and detection of single strand breaks as well as DNA breaks by exposure to alkali [54].…”

Section: Discussionmentioning

confidence: 99%

“…T. foetus parasites were cultivated as previously described and evaluated following the Single Cell Gel Electrophoresis/Comet Assay (SCGE/Comet Assay) protocol used by [36] and described by [37]. The number of control and PDT-treated cells was adjusted for 1 × 10 4 .…”

Section: Single Cell Gel Electrophoresis/comet Assaymentioning

confidence: 99%

“…Mean tail moments (tail length × tail DNA%) were automatically scored using the Open-Comet analysis software. Two-tailed Student t-tests were used for data statistical analysis and software Prism (GraphPad Software) for representation [36,38,39].…”

Section: Single Cell Gel Electrophoresis/comet Assaymentioning

confidence: 99%

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DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

Margraf-Ferreira

Carvalho

Machado

et al. 2017

Photodiagnosis and Photodynamic Therapy

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Photodynamic therapy (PDT) is a modality of therapy that involves the activation of photosensitive substances and the generation of cytotoxic oxygen species and free radicals to promote the selective destruction of target tissues. This study analyzed the application of PDT to Tritrichomonas foetus, a scourged and etiological agent of bovine trichomoniasis, a sexually transmitted infectious disease. As it is an amitochondrial and aerotolerant protozoan, it produces energy under low O tension via hydrogenosome. T. foetus from an axenic culture was incubated with photosensitizer tetrasulfonated aluminium phthalocyanine and then irradiated with a laser source (InGaAIP) at a density of 4.5Jcm. The DNA integrity of the control and treated group parasites was analyzed by conventional gel electrophoresis and comet assay techniques. In previous results, morphological changes characterized by apoptotic cell death were observed after T. foetus was submitted to PDT treatment. In the treated groups, T. foetus DNA showed a higher concentration of small fragments, about 200pb, in gel electrophoresis after PDT. In the comet assay, the DNA tail percentage was significantly higher in the treated groups. These results demonstrate that PDT leads to DNA fragmentation with changes in nuclear morphology and apoptotic features.

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Section: Discussionmentioning

confidence: 99%

Section: Single Cell Gel Electrophoresis/comet Assaymentioning

confidence: 99%

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DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

Margraf-Ferreira

Carvalho

Machado

et al. 2017

Photodiagnosis and Photodynamic Therapy

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“…These data were chosen based on Gyori et al, 25 who explained the OpenComet software. The comet assay protocol was described by Singh et al and used with a minor modification described in Silva and performed by Carvalho et al [26][27][28] Micronucleus assay…”

Section: Comet Assaymentioning

confidence: 99%

Genotoxic effects of photodynamic therapy in laryngeal cancer cells – Anin vitrostudy

Moraes

Godoi

Carvalho

et al. 2019

Exp Biol Med (Maywood)

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Photodynamic therapy provides the formation of reactive oxygen species that are capable of inducing cell death. Human laryngeal carcinoma (HEp-2) cells have been evaluated in this study under PDT treatment. Cells were treated with photosensitizer aluminum phthalocyanine tetrasulfonate (AlPcS4) and irradiated with a Biopdi/Irrad-Led5 660 LED with 660 nm wavelength, intensity of delivered light of 25 mW/cm2, power of 70 mW, fluence of 5 J/cm2 for 24 h and 48 h, and then evaluated. Cell population was not increased by PDT treatment after the tested period. The apoptosis assay demonstrated that control groups exhibit approximately 60% of living cells in the 24 h and 48 h periods, however. A significant increase in apoptotic cells was observed after the photodynamic therapy treatments, for both 24 and 48 h groups. Over 50% of cells were under apoptosis after photodynamic therapy, evidencing a death process generated from the oxidative damage of the treatment. Comet assay and micronucleus assessments, both of which evaluate genotoxicity, demonstrated favorable results to damages caused by the photodynamic therapy treatment. Thus, photodynamic therapy is proposed to damage nuclear cells and the subcellular structure of carcinogenic cells. Impact statement Recently, the use of photodynamic therapy grows as an alternative treatment for cancer, since it has a noninvasive characteristic and affinity to the tumor tissue. Accordingly, understanding the therapy’s foci of action is important for the technique improvement. This work aims to understand the genotoxic effect triggered by the therapy action, thus evidencing the permanent changes caused to the genetic material of the tumor cell after the treatment. Therefore, to increase the knowledge in this study field, the methodology of the comet assay and count of micronucleus formed after the therapy was adopted in order to understand if the damage caused to the DNA of tumor cell makes its replication process unfeasible in future generations. The study allows a better therapeutic approach to the cancer treatment, making the process of association between therapies a more effective option during the disease treatment.

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“…The experimental feeding regime is illustrated in Fig. 1 and is described in detail elsewhere (23). This study was approved by the Ethical Committee on Research at São Paulo State University (UNESP) (protocol no.…”

Section: Animalsmentioning

confidence: 99%

Prenatal alcohol exposure reduces mandibular calcium and phosphorus concentrations in newborn rats

et al. 2016

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Previous studies suggest that prenatal alcohol exposure affects fetal bone development, including bone quality. This study evaluated the chemical composition of mandibles from newborn rats after maternal 20% alcohol consumption before and throughout gestation. Nine rats were initially distributed into three groups: an Alcohol group, Pairfed group, and Control group. The groups were fed prespecified diets for 8 weeks before and the 3 weeks during pregnancy. At age 5 days, eight newborns from each group were euthanized (total, n = 24). Using energy dispersive spectrometry, we evaluated samples of mandibles from newborns to identify changes in bone mineralization, specifically Ca and P concentrations. Ca and P concentrations were lower in the Alcohol group than in the Control and Pair-fed groups (P = 0.003 and P = 0.001, respectively). In summary, alcohol exposure before and throughout gestation reduces mandibular Ca and P concentrations in newborn rats. (J Oral Sci 58, 439-444, 2016)

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High doses of alcohol during pregnancy cause DNA damages in osteoblasts of newborns rats

Cited by 12 publications

References 66 publications

DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

DNA analysis of cattle parasitic protozoan Tritrichomonas foetus after photodynamic therapy

Genotoxic effects of photodynamic therapy in laryngeal cancer cells – Anin vitrostudy

Prenatal alcohol exposure reduces mandibular calcium and phosphorus concentrations in newborn rats

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