High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver

Abstract

(Sub)chronic supplementation of sedentary adolescent male rats with high TE doses does not exert a lasting major effect on the liver antioxidant barrier and redox homeostasis.

High-dose testosterone supplementation disturbs liver pro-oxidant/antioxidant balance and function in adolescent male Wistar rats undergoing moderate-intensity endurance training

“…The higher TE dose significantly lowered BW gain and LW as compared to those in TE-untreated EndTr rats, but caused no substantial shift in the LW/BW ratio (−5%, p = 0.66) and thus no apparent hepatotoxicity in EndTr rats. Earlier, we found a significantly reduced LW/BW ratio in high-dose TE-treated adolescent UTr rats (Sadowska-Krępa et al, 2017), which may have been due to the start of TE treatment at a younger age. The training was performed at an intensity that allows maximal fat oxidation (Purdom et al, 2018), and the mean BW gains were less in all three EndTr groups than in the respective UTr groups, see Sadowska-Krępa et al (2017).…”

“…All biochemical determinations in blood serum and liver samples, except for serum lipids that were not determined before, were performed as described earlier (Sadowska-Krępa et al, 2017). Specifically: The total serum testosterone (TT) level was assessed with a DSL-4100 Testosterone RIA Kit (Diagnostic Systems Laboratories, Webster, TX, USA).…”

“…All other data were analyzed by a one-way ANOVA with weekly TE dose as the main factor, followed by the Tukey test when appropriate. Comparisons with data from our previous study (Sadowska-Krępa et al, 2017) were made using Student's t -test for independent variables, as indicated in the text. Associations between variables were assessed using the Spearman rank correlation test.…”

“…Our earlier study on the effects of long-term testosterone treatment on the liver antioxidant barrier and some blood markers of liver injury in sedentary adolescent male rats showed some signs of enhanced liver oxidative stress and toxicity but no potential lasting harm (Sadowska-Krępa et al, 2017). Here, we tested the possible harmful effects of this treatment in a situation aimed to model androgen abuse to aid physical exercise training.…”

Study on the mechanism and therapeutic effect of Antelope horn treatment on cerebral ischemia reperfusion injury of mouse

“…Ischemic pre-conditioning and post-conditioning can induce increased expression of Hsp 27, which can protect the tissue from ischemia reperfusion injury, thus constituted an endogenous protective mechanism. Studies have reported that Hsp 27 can protect cerebral ischemia reperfusion injury (Sadowska-Krępa et al, 2017), increased Hsp 27 could reduce azithromycin-induced cardiac cell death (Kumar et al, 2016), and Hsp 27 can alleviate cerebral ischemia reperfusion injury (Mohammadi-Ostad-Kalayeh et al, 2017). This research found that, compared with the ischemic control group, Hsp 27 and Hsp 27 levels increased significantly after medication, suggesting that the protective effect of Antelope horn on cerebral ischemia reperfusion is correlated with the elevation of Hsp 27 and Hsp 70.…”