High dose of dietary vitamin D3 modulated the yellow catfish (Pelteobagrus fulvidraco) splenic innate immune response after Edwardsiella ictaluri infection

Cheng, Ke; Tang, Qin; Guo, Xun; Karow, Niel A.; Wang, Chunfang

doi:10.1016/j.fsi.2020.03.005

Cited by 19 publications

(11 citation statements)

References 52 publications

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“…In fish, dietary VD can increase the activity of serum peroxidase in sea bream ( Sparus aurata L. ) [ 20 ] and European sea bass ( Dicentrarchus labrax L. ) [ 40 ]. In addition, previous studies in our lab have also found that dietary VD 3 or cell culture medium supplemented with 1,25(OH) 2 D 3 can improve the antioxidant capacity of yellow catfish or head kidney macrophages and reduce the production of ROS [ 23 , 24 , 25 ]. All this evidence underscores the role of VD 3 in resistance to lipid peroxidation and ferroptosis in animals.…”

Section: Discussionmentioning

confidence: 90%

“…In fish, VD 3 was first recognized to regulate the metabolism and balance of calcium and phosphorus. However, recent studies have found that VD 3 can also regulate innate immunity and exert antioxidant function [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ]. Furthermore, VD has also been reported to regulate iron metabolism and to be a modulator of the hepcidin–ferroportin axis in humans and mammals [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…Based on the previous experimental results from our laboratory, the supplementation of 1,25(OH) 2 D 3 in cell culture medium reduced ROS production induced by lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C)) in yellow catfish head kidney macrophages, while supplementation of VD 3 in diets significantly lowered the deposition of hemosiderin in yellow catfish spleen after Edwardsiella ictaluri challenge. Moreover, dietary VD 3 affected the ‘iron ion transport’ signaling pathway in yellow catfish immune tissues based on the next-generation sequencing results [ 23 , 24 , 25 ]. However, information about the relationship between VD and ferroptosis has not been well documented.…”

Section: Introductionmentioning

confidence: 99%

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1,25(OH)2D3 Inhibited Ferroptosis in Zebrafish Liver Cells (ZFL) by Regulating Keap1-Nrf2-GPx4 and NF-κB-hepcidin Axis

Cheng

Huang

Wang

2021

IJMS

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Ferroptosis is a kind of iron-dependent programed cell death. Vitamin D has been shown to be an antioxidant and a regulator of iron metabolism, but the relationship between vitamin D and ferroptosis is poorly studied in fish. This study used zebrafish liver cells (ZFL) to establish a ferroptosis model to explore the effect of 1,25(OH)2D3 on cell ferroptosis and its mechanism of action. The results showed that different incubation patterns of 1,25(OH)2D3 improved the survival rate of ZFL, mitigated mitochondrial damage, enhanced total glutathione peroxidase (GPx) activity, and reduced intracellular reactive oxygen species (ROS), lipid peroxidation (LPO), and malondialdehyde (MDA), as well as iron ion levels, with the best effect at 200 pM 1,25(OH)2D3 preincubation for 72 h. Preincubation of ZFL at 200 pM 1,25(OH)2D3 for 72 h downgraded keap1 and ptgs2 gene expression, increased nrf2, ho-1, fth1, gpx4a,b expression, and lowered the expression of the nf-κb p65,il-6,il-1β gene, thus reducing the expression of hamp1. The above results indicate that different incubation patterns of 1,25(OH)2D3 have protective effects on ferroptosis of ZFL induced by ferroptosis activator RSL3 and 1,25(OH)2D3 can inhibit ferroptosis of ZFL by regulating Keap1–Nrf2–GPx4 and NF-κB–hepcidin axis.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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1,25(OH)2D3 Inhibited Ferroptosis in Zebrafish Liver Cells (ZFL) by Regulating Keap1-Nrf2-GPx4 and NF-κB-hepcidin Axis

Cheng

Huang

Wang

2021

IJMS

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“…In the process of previous research on the innate immunity of fish, the liver and spleen of teleost fishes were used as the key research objects (Dang et al ., 2016, 2017, 2018a, 2018b), and in previous studies on the innate immune response of yellow catfish, the liver and spleen were also the focus of research (Cheng et al ., 2020; Tang et al ., 2021). To study the role of trafs in the immune response of yellow catfish, the expression patterns of traf1‐7 in the liver and spleen under A. hydrophila infection were analysed.…”

Section: Resultsmentioning

confidence: 99%

“…studies on the innate immune response of yellow catfish, the liver and spleen were also the focus of research (Cheng et al, 2020;Tang et al, 2021). To study the role of trafs in the immune response of yellow catfish, the expression patterns of traf1-7 in the liver and spleen under A. hydrophila infection were analysed.…”

Section: Fishmentioning

confidence: 99%

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Nie

Wang

Zhang

et al. 2022

Journal of Fish Biology

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Tumour necrosis factor (TNF) receptor‐associated factor (TRAF) is a receptor protein that has important functions in the immune system. Nonetheless, there have been few reports of traf genes in teleost fishes. The present study aimed to identify the traf genes from the genomic information of yellow catfish (Pelteobagrus fulvidraco). Eight traf genes were identified and named, which are distributed on different chromosomes but have similar conserved protein domains. Phylogenetic and syntenic analyses demonstrated conservation of traf genes during evolution. In addition, yellow catfish has the relatively rare traf1 and traf5 genes. Gene structure and motif analysis revealed the homology and distribution diversity of the traf genes. Quantitative real‐time reverse transcription PCR was used to study the expression patterns of traf genes in healthy fish tissues and after infection by Aeromonas hydrophila. The results demonstrated significant changes in traf gene expression, indicating a potential role in innate immunity.

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Vitamin D—‘Keep the Orthopedist Away!’

Steinberg

2022

Aquatic Animal Nutrition

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High dose of dietary vitamin D3 modulated the yellow catfish (Pelteobagrus fulvidraco) splenic innate immune response after Edwardsiella ictaluri infection

Cited by 19 publications

References 52 publications

1,25(OH)2D3 Inhibited Ferroptosis in Zebrafish Liver Cells (ZFL) by Regulating Keap1-Nrf2-GPx4 and NF-κB-hepcidin Axis

1,25(OH)2D3 Inhibited Ferroptosis in Zebrafish Liver Cells (ZFL) by Regulating Keap1-Nrf2-GPx4 and NF-κB-hepcidin Axis

Genome‐wide identification of the traf gene family in yellow catfish (Pelteobagrus fulvidraco) and analysis of their expression in response to bacterial challenge

Vitamin D—‘Keep the Orthopedist Away!’

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