High diversity in SCCmec elements among multidrug-resistant Staphylococcus haemolyticus strains originating from paediatric patients; characterization of a new composite island

Hosseinkhani, Farideh; Buirs, Matthias Tammes; Jabalameli, Fereshteh; Emaneini, Mohammad; Leeuwen, Willem B. van

doi:10.1099/jmm.0.000776

Cited by 13 publications

(11 citation statements)

References 35 publications

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“…This result is consistent with a previous study indicating that MRSH is prevalently detected in inpatients accounting for 83.6% (Ternes et al 2013). Moreover, MRSH has been identified as the dominant player in catheter-linked bacteremia in the intensive care unit patients (Rodríguez-Aranda et al 2009) including neonatal and pediatric patients (Hosseinkhani et al 2018;Ternes et al 2013). Furthermore, other studies also revealed the high survival and adaptive potential of MRSH strains within healthcare environment as they are detected and cross-transmission not only through medical devices (Kim et al 2012), but also patients and hospital staff (Squeri et al 2012).…”

Section: Discussionsupporting

confidence: 92%

Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia

2019

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Abstract. Suhartono S, Hayati Z, Mahmuda Mahmuda M. 2019. Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia. Biodiversitas 20: 2076-2080. The occurrence of Staphylococcus-related infections is emerging and might potentially harbor multidrug resistance leading to the major risks of hospital-associated infection. The study aimed to determine the dominant species distributed among Staphylococcus-related infections from clinical specimens and determine antibiotic resistance profile of the dominant species. The clinical samples were collected from inpatients and outpatients at the Zainoel Abidin Regional Hospital of Aceh, Indonesia during March 2017-March 2018. All clinical samples were mainly inoculated to plates containing blood agar followed by identification and determination of their antibiotic susceptibility using VITEK® 2 Compact. Statistical analysis was performed using Chi-square test or Fisher's exact test when appropriate to determine the independence of frequency distributions, and the tests were considered statistically significant at a P ≤ 0.05 on two-tailed. Of 693 Staphylococcus isolates found in the clinical specimens, Staphylococcus haemolyticus was the most predominant isolates with a total of 233 (32.2%), and it was identified with a high prevalence of methicillin-resistance (95.96%) termed as methicillin-resistant Staphylococcus haemolyticus (MRSH). MRSH were found in the blood samples and ICU patients accounting for 64.1% and 55.61%, respectively. The resistance profile of MRSH isolates exhibited a high level of resistance (more than 85%) to a wide range of antibiotics, including beta-lactams, (first to the fourth generation) cephalosporins, fluoroquinolones, carbapenems, and macrolides. This might be an alarming occurrence and require serious action to prevent MRSH dissemination of healthcare infections in the future.

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Section: Discussionsupporting

confidence: 92%

Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia

2019

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“…Currently, vancomycin, linezolid and rifampicin are the treatment of choice for Staphylococcus haemolyticus infections 14, 21 , our results showed that resistance to vancomycin and linezolid do not exist, particularly with the absence of vanA gene (0% in this study) which in agreement with what reported worldwide, however, resistance to rifampicin exhibited by 30% of the isolates reported in this study is alarming, emerging Staphylococcus haemolyticus resistant to rifampicin have been reported 7 , Resistance to glycopeptides (vancomycin and teicoplanin) remains rarely detected in Staphylococcus haemolyticus 3, 6, 14, 16, 18 . Despite the total absence of vancomycin/teicoplanin, linezolid resistant isolates and the apparently low prevalence of rifampicin resistance among Staphylococcus haemolyticus reported in this study, wide surveillance of susceptibility patterns of these drugs among Staphylococcus haemolyticus of clinical origin is mandatory, since the development of resistance to these drugs may exist at low levels 17 .…”

Section: Resultssupporting

confidence: 89%

First Report of Multi-drug Resistant Staphylococcus haemolyticus in Nosocomial Infections in North Western Saudi Arabia

Alahmadi¹,

Alahmadey²,

Organji³

et al. 2021

J Pure Appl Microbiol

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We report in this study for the first time the prevalence of multiple resistant Staphylococcus haemolyticus in clinical settings in Saudi Arabia. A total of 1060 clinical specimens of hospitalized patients were screened for the presence of S. haemolyticus in the period between September and December 2020. Primary identification of the isolates was carried out by colonial characteristics on mannitol salt agar and clumping factor test, confirmation of presumptive isolates and antimicrobial susceptibility testing was performed by Vitek® 2, while PCR was employed to detect mecA and vanA genes. A total of 20 S. haemolyticus isolates were recovered from 20 samples (blood cultures, urine, nasal swab, wound swab, groin swab, and axilla swab), 90% (P <0.001, x2) of the isolates were multiple resistant to three antimicrobial agents and more. Resistance to oxacillin was exhibited in 95% of the isolates, while none of the isolates were resistant to vancomycin and linezolid, yet resistance to rifampicin was observed in 30 % of the isolates. The findings of this study highlights the emerging trends of Staphylococcus haemolyticus as potential drug resistant pathogen in hospital settings in Saudi Arabia, which requires in depth investigation on molecular understanding on antimicrobial resistance and virulence traits of the strains.

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“…Some Staphylococcus strains are considered inhabitants of the normal microbiota, and do not have all the virulence factors found in S. aureus. Their emerging threat comes from the fact that these bacteria can carry a large number of antimicrobial resistance genes [15], [16]. Due to the increase in antibiotic resistance in the recent years as a result of the use of these antibiotics, the World Health Organization announced a "global action plan on antimicrobial resistance" [17].…”

Section: Resultsmentioning

confidence: 99%

Antibiotic Resistance of Staphylococcus spp. Isolated from Sewage in Manaus, Amazonas

Nogueira

Andrade

Magalhães

et al. 2021

EJMED

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Staphylococcus spp. have become important human pathogens in recent decades due to the selection of resistant bacteria and the spread of their resistance genes in the environment. This study aimed to evaluate the resistance of Staphylococcus spp. obtained from sewage in the city of Manaus, Amazonas state, Brazil. The isolates were tested for susceptibility to antimicrobials using the Kirby-Bauer method for ampicillin, azithromycin, ciprofloxacin, clindamycin, chloramphenicol, erythromycin, gentamicin, oxacillin, cefoxitin, linezolid, penicillin, rifampicin, sulfazotrim, tetracycline and vancomycin. Among the strains isolated from sewage, the greatest resistance was observed for penicillin and oxacillin, with 100% of isolates resistant to these antibiotics. Some antibiotics had resistant and sensitive strains (ampicillin, clindamycin, erythromycin, cefoxitin, azithromycin). Staphylococcus spp. were identified as sensitive to ciprofloxacin chloramphenicol gentamicin linezolid sulfazotrim tetracycline, vancomycin, with no strain resistant to these antibiotics.

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High diversity in SCCmec elements among multidrug-resistant Staphylococcus haemolyticus strains originating from paediatric patients; characterization of a new composite island

Cited by 13 publications

References 35 publications

Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia

Distribution of Staphylococcus haemolyticus as the most dominant species among Staphylococcal infections at the Zainoel Abidin Hospital in Aceh, Indonesia

First Report of Multi-drug Resistant Staphylococcus haemolyticus in Nosocomial Infections in North Western Saudi Arabia

Antibiotic Resistance of Staphylococcus spp. Isolated from Sewage in Manaus, Amazonas

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