High-Density Culture of Human Islets on Top of Silicone Rubber Membranes

Papas, Klearchos K.; Avgoustiniatos, Efstathios S.; Tempelman, Linda A.; Weir, Gordon C.; Colton, Clark K.; Pisania, Anna; Rappel, Michael J.; Friberg, Andrew; Bauer, Andréa Carla; Hering, Bernhard J.

doi:10.1016/j.transproceed.2005.09.086

Cited by 53 publications

(67 citation statements)

References 8 publications

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“…For experiments at 20% and 3% O 2 , islets were placed in 100 cm 2 silicone rubber flasks 51 (PR-AY-5-0017-5; Wilson Wolf Manufacturing) in islet culture media with a depth of 2.5 mm and a surface density of <30 IE/cm 2 . The flasks were placed in a sealed humidified chamber that was continually flushed with 20% or 1% O 2 , 5% CO 2 , and the balance N 2 and was in turn placed within an incubator at 378C.…”

Section: Culture Of Encapsulated Tissuementioning

confidence: 99%

Quantitative Assessment of Islets of Langerhans Encapsulated in Alginate

Johnson

O'Sullivan

D'Aoust

et al. 2011

Tissue Engineering Part C: Methods

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Section: Culture Of Encapsulated Tissuementioning

confidence: 99%

Quantitative Assessment of Islets of Langerhans Encapsulated in Alginate

Johnson

O'Sullivan

D'Aoust

et al. 2011

Tissue Engineering Part C: Methods

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“…However, use of colder temperatures than 228C has not been well characterized with regard to islet viability and recovery for clinical purposes. Alternatively, culture and transport of islets could be performed in gas-permeable devices [24]. It is, however, thereby crucial to avoid focal islet aggregation or accumulation to ensure optimal oxygenation of islets.…”

Section: Oxygenation Of Isolated Isletsmentioning

confidence: 99%

Oxygenation of islets and its role in transplantation

Lau¹,

Henriksnäs²,

Svensson³

et al. 2009

Current Opinion in Organ Transplantation

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“…11 Noninvasive monitoring of the oxygen partial pressure (pO 2 ) within TEGs is critically important because of the deleterious effects of long-and short-term exposure of islet tissue to hypoxia or anoxia. 2,7,8,[11][12][13] Lack of oxygen is the most critical issue preventing widespread utilization of macroencapsulated islet transplantation, and delivery of exogenous oxygen has been shown to enhance TEG survival and function in vivo. 2,14,15 Noninvasive monitoring of pO 2 can improve TEG development and allow the evaluation of TEG viability 16 and local changes in pO 2 with the delivery of supplemental oxygen (DSO).…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Noninvasive Magnetic Resonance Relaxometry for the In Vivo Assessment of Tissue-Engineered Graft Oxygenation

Einstein

Weegman

Firpo

et al. 2016

Tissue Engineering Part C: Methods

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Techniques to monitor the oxygen partial pressure (pO 2 ) within implanted tissue-engineered grafts (TEGs) are critically necessary for TEG development, but current methods are invasive and inaccurate. In this study, we developed an accurate and noninvasive technique to monitor TEG pO 2 utilizing proton ( 1 H) or fluorine ( 19 F) magnetic resonance spectroscopy (MRS) relaxometry. The value of the spin-lattice relaxation rate constant (R 1 ) of some biocompatible compounds is sensitive to dissolved oxygen (and temperature), while insensitive to other external factors. Through this physical mechanism, MRS can measure the pO 2 of implanted TEGs. We evaluated six potential MRS pO 2 probes and measured their oxygen and temperature sensitivities and their intrinsic R 1 values at 16.4 T. Acellular TEGs were constructed by emulsifying porcine plasma with perfluoro-15-crown-5-ether, injecting the emulsion into a macroencapsulation device, and cross-linking the plasma with a thrombin solution. A multiparametric calibration equation containing R 1 , pO 2 , and temperature was empirically generated from MRS data and validated with fiber optic (FO) probes in vitro. TEGs were then implanted in a dorsal subcutaneous pocket in a murine model and evaluated with MRS up to 29 days postimplantation. R 1 measurements from the TEGs were converted to pO 2 values using the established calibration equation and these in vivo pO 2 measurements were simultaneously validated with FO probes. Additionally, MRS was used to detect increased pO 2 within implanted TEGs that received supplemental oxygen delivery. Finally, based on a comparison of our MRS data with previously reported data, ultra-high-field (16.4 T) is shown to have an advantage for measuring hypoxia with 19 F MRS. Results from this study show MRS relaxometry to be a precise, accurate, and noninvasive technique to monitor TEG pO 2 in vitro and in vivo.

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High-Density Culture of Human Islets on Top of Silicone Rubber Membranes

Cited by 53 publications

References 8 publications

Quantitative Assessment of Islets of Langerhans Encapsulated in Alginate

Quantitative Assessment of Islets of Langerhans Encapsulated in Alginate

Oxygenation of islets and its role in transplantation

Development and Validation of Noninvasive Magnetic Resonance Relaxometry for the In Vivo Assessment of Tissue-Engineered Graft Oxygenation

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