High Affinity Iron Acquisition Systems Facilitate but Are Not Essential for Colonization of Chickens by Salmonella Enteritidis

Wellawa, Dinesh H.; Lam, Po-King S.; White, Aaron P.; Gomis, Susantha; Allan, Brenda; Köster, Wolfgang

doi:10.3389/fmicb.2022.824052

Cited by 3 publications

(2 citation statements)

References 89 publications

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“…Our previous report showed that “active” colonization of the cecum with SEn strain LS101 occurred between 24 and 48 h p.i. in day-old birds challenged with the 10 9 CFU dose ( 21 ). In that study, the cecal Salmonella burden reached a maximum of ~10 9 CFU/g 24 and 48 h p.i.…”

Section: Discussionmentioning

confidence: 99%

“…In parallel, an experiment from our previous study provided evidence that yolk sac infection was not visualized prior to day 2 p.i. by LS101 sig70c35 [Supplementary Figure 4 of ( 21 )]. The passage of the yolk stalk gets partially occluded by lymphocyte proliferation minimizing the transfer of material between the yolk and the jejunum ( 25 ).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

et al. 2022

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The light emitting module lux operon (luxCDABE) of Photorhabdus luminescens can be integrated into a “dark” bacterium for expression under a suitable promoter. The technique has been used to monitor kinetics of infection, e.g., by studying gene expression in Salmonella using mouse models in vivo and ex vivo. Here, we applied the bioluminescence imaging (BLI) technique to track Salmonella Enteritidis (SEn) strains carrying the lux operon expressed under a constitutive promoter sequence (sigma 70) in chicken after oral challenge. Detectable photon signals were localized in the crop, small intestine, cecum, and yolk sac in orally gavaged birds. The level of colonization was determined by quantification of signal intensity and SEn prevalence in the cecum and yolk sac. Furthermore, an isogenic SEn mutant strain tagged with the lux operon allowed for us to assess virulence determinants regarding their role in colonization of the cecum and yolk sac. Interestingly, mutations of SPI-1(Salmonella Pathogenicity Island 1) and fur (ferric uptake regulator) showed significantly decreased colonization in yolk sac that was correlated with the BLI data. A similar trend was detected in a ΔtonB strain by analyzing enrichment culture data. The inherently low quantum yield, light scattering, and absorption by tissues did not facilitate detection of signals from live birds. However, the detection limit of lux operon has the potential to be improved by resonance energy transfer to a secondary molecule. As a proof-of-concept, we were able to show that sensitization of a fluorescent-bound molecule known as the lumazine protein (LumP) improved the limit of detection to a certain extent.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

et al. 2022

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Characterization and Statistical Optimization of Enterobatin Synthesized by Escherichia coli OQ866153

Khazaal,

Faraag,

Hamada

et al. 2024

Biochem Genet

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Microorganisms produce siderophores, which are secondary metabolites with a high affinity for iron. Siderophores have received significant attention due to their diverse applications in ecological and clinical research. In this study, siderophores production by Escherichia coli OQ866153 was optimized using two-stage statistical approach involving Plackett–Burman design (PBD) and response surface methodology (RSM) using central composite design (CCD). Out of 23 variables, succinate, tryptophan, Na2HPO4, CaCl2, agitation, and KH2PO4 were found to have the most significant effect on siderophores production in the first optimization stage with the highest SU% of 43.67%. In the second stage, RSM using CCD was utilized, and the optimal conditions were determined to be 0.3 g/l succinate, 0 g/l tryptophan, 6 g/l Na2HPO4, 0.1 g/l CaCl2, 150 RPM agitation, and 0.6 g/l KH2PO4, resulting in a maximum siderophore units (SU%) of 89.13%. The model was significant, as indicated by the model f-value of 314.14 (p-value = 0.0004) and coefficient of determination R2 of 0.9950. During validation experiments, the obtained maximum SU% was increased up to 87.1472%, which was two times as the value obtained under ordinary conditions (46.62%). The produced siderophores were purified and characterized using 1H, 13C NMR, IR spectroscopy. The obtained results indicated that the compound was enterobactin and entABCDEF genes were further detected in Escherichia coli OQ866153 extracted DNA. To our knowledge, this is the first report of statistical optimization for enterobactin synthesis by an E. coli strain isolated from a clinical source in Egypt.

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Role of Stress-Induced Proteins RpoS and YicC in the Persistence of Salmonella enterica subsp. enterica Serotype Typhimurium in Tomato Plants

Deblais

Ranjit

Vrisman

et al. 2023

MPMI

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Understanding the functional role of bacterial genes in the persistent of Salmonella in plant organs can facilitate the development of agricultural practices to mitigate food safety risks associated with the consumption of fresh produce contaminated with Salmonella. Our study showed that Salmonella enterica subsp. enterica serotype Typhimurium (strain MDD14) persisted less in inoculated tomato plants than other S. Typhimurium strains tested (JSG210, JSG626, JSG634, JSG637, JSG3444, and EV030415; P<0.01). In vitro assays performed in limited-nutrient conditions (growth rate, biofilm production and motility) were inconclusive in explaining the in planta phenotype observed with MDD14. Whole genome sequencing combined with non-synonymous single nucleotide variations (nsSNVs) analysis was performed to identify genomic differences between MDD14 and the other S. Typhimurium strains. The genome of MDD14 contained a truncated version (123 bp N-terminal) of yicC and a mutated version of rpoS (two non-synonymous substitutions; G66E and R82C), which are two stress induced proteins involved in iron acquisition, environmental sensing and cell envelop integrity. The rpoS and yicC genes were deleted in S. Typhimurium JSG210 with the Lambda Red recombining system. Both mutants had limited persistence in tomato plant organs, similar to MDD14. In conclusion, we demonstrated that YicC and RpoS are involved in the persistence of Salmonella in tomato plants in greenhouse conditions, and thus, could represent potential targets to mitigate persistence of Salmonella spp. in planta.

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High Affinity Iron Acquisition Systems Facilitate but Are Not Essential for Colonization of Chickens by Salmonella Enteritidis

Cited by 3 publications

References 89 publications

Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

Characterization of colonization kinetics and virulence potential of Salmonella Enteritidis in chickens by photonic detection

Characterization and Statistical Optimization of Enterobatin Synthesized by Escherichia coli OQ866153

Role of Stress-Induced Proteins RpoS and YicC in the Persistence of Salmonella enterica subsp. enterica Serotype Typhimurium in Tomato Plants

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