Hierarchical Incorporation of Surface-Functionalized Laponite Clay Nanoplatelets with Type I Collagen Matrix

Abstract

Unraveling the interaction mechanisms of type I collagen with various inorganic nanoparticles is of pivotal importance to construct collagen-based bionanocomposites with hierarchical structures for biomedical, pharmaceutical, and other industrial applications. In this study, synthetic two-dimensional Laponite nanoplatelets (LAP NPs) are surface-functionalized with tetrakis­(hydroxymethyl) phosphonium sulfate (THPS) for reinforcing their incorporation with type I collagen matrix by focusing on the influences of the interactions on the hierarchical structures of the collagen. Our results indicate that the LAP NPs can be successfully surface-functionalized with THPS via covalent bonds between the amine-functionalized NPs and the hydroxymethyl groups of THPS. Moreover, the resulting NPs can be well dispersed into the collagen matrix and evenly bound onto the collagen fiber strands and between the collagen fibrils, preserving the native D-periodic banding patterns of the collagen fibrils. The formation of covalent and hydrogen bonds between the collagen and the functionalized NPs can stabilize the intrinsic triple-helical conformation of the collagen, conferring the resulting collagen-based nanocomposites with improved thermal stability and enhanced mechanical properties. We anticipate that a fundamental understanding of the interactions between the collagen and functionalized inorganic nanoparticles would contribute to the design, fabrication, and further application of hierarchical collagen-based bionanocomposites with multifunctions.

Developing Ecofriendly Chrome-Free Leather Processing-Based Polymer-Intercalated Layered Double Hydroxide Supramolecules

“…It can be seen that the isoelectric point (IEP) of leather tanned by LDHs, P-LDHs, and GEP-LDHs increased from 5.4 of pickled pelt to 6.8, 6.3, and 6.6, respectively, confirming that the positively charged LDHs, P-LDHs, and GEP-LDHs can bind with the carboxyl group of collagen molecules through electrostatic interaction. 44 XPS is employed to further study the interaction mechanism of LDHs, P-LDHs, respectively. These results indicate the improved hydrothermal stability and thermal stability of LDH-, P-LDH-, and GEP-LDH-tanned leather, and the hydrothermal stability and thermal stability of GEP-LDH-tanned leather is higher than that of LDH-and P-LDH-tanned leather, owing to the uniform distribution of GEP-LDHs in collagen fibers and the excellent cross-linking ability between them.…”

A comparative study of bone bioactivity and osteogenic potential of different bioceramics in methacrylated collagen hydrogels

“…Change The overall significance of this study is in the direct comparison of bioceramic performance on bone bioactivity and growth factor-free osteogenic differentiation of MSCs toward the identification of the most potent bioceramic material for use in the biomimetic design and development of composite scaffolds for BTE applications. While biomimetic scaffolds that combine collagen and bioceramic materials have been extensively studied in prior literature, [19][20][21][22] a single study that compares bioceramic performance head-to-head has not yet been undertaken. In this work, CMA was combined with different bioceramics to form composite hydrogels and a head-to-head comparison of bioceramic type on the physical properties, mechanical properties, and bone bioactivity of CMA hydrogels were investigated.…”

“…Furthermore, bioceramics have been often combined with collagen to generate mechanically tough scaffolds with compositional properties akin to native bone. These composite scaffolds have been shown to provide the essential biophysical and biochemical cues to guide cell proliferation, differentiation, and de novo mineralization 15–18 . In this realm, a plethora of different bioceramics such as hydroxyapatite, bioactive glasses, tricalcium phosphates, and nanosilicates have been used in the development of composite scaffolds for growth factor‐free strategies for BTE applications 19–22 .…”

“…These composite scaffolds have been shown to provide the essential biophysical and biochemical cues to guide cell proliferation, differentiation, and de novo mineralization. [15][16][17][18] In this realm, a plethora of different bioceramics such as hydroxyapatite, bioactive glasses, tricalcium phosphates, and nanosilicates have been used in the development of composite scaffolds for growth factor-free strategies for BTE applications. [19][20][21][22] However, a head-to-head comparison of the different bioceramics on the physical properties of collagenbased scaffolds and osteogenic differentiation of MSCs has not yet been performed.…”

“…[15][16][17][18] In this realm, a plethora of different bioceramics such as hydroxyapatite, bioactive glasses, tricalcium phosphates, and nanosilicates have been used in the development of composite scaffolds for growth factor-free strategies for BTE applications. [19][20][21][22] However, a head-to-head comparison of the different bioceramics on the physical properties of collagenbased scaffolds and osteogenic differentiation of MSCs has not yet been performed. Moreover, prior work has mostly entailed seeding cells directly on bioceramic granules 23 or atop a composite scaffold, 24 but a comparative investigation of bioceramic performance with encapsulated cells is lacking.…”