Hierarchical Dendritic Mesoporous TiO₂ Nanocomposites for Highly Selective Enrichment of Endogenous Phosphopeptides

Abstract

The uniform growth of metal oxides into mesoporous nanostructures with controllable orientations is challenging but of significance. Here, a polydopamine (PDA)-induced oriented growth strategy is proposed to uniformly grow metal ions into mesoporous metal oxides with controllable orientation. Two mesoporous nanostructures (3D hierarchical dendritic nanostructures and 2D nanosheets) were constructed by this strategy. It is worth emphasizing that the 3D hierarchical mesoporous nanostructures (DMSNs@TiO2) are formed by constructing single-layered porous TiO2 in dendritic mesoporous tunnels, which is different from the previously reported generation of hierarchical pores inside frameworks or accumulation of various channels. In such a pore system, small pores are connected by large pores, thereby enhancing the accessibility of pores. DMSNs@TiO2 with a large surface area and connected hierarchical pore structure exhibit highly improved phosphopeptide enrichment performance compared with commercial TiO2 and single-pored TiO2. Especially for real samples such as human saliva, DMSNs@TiO2 captured 49 endogenous phosphopeptides from 1 μL of saliva, demonstrating their broad prospects in phosphopeptidomics analysis.

Construction and application of carbon aerogels in microwave absorption

“…115 To construct hierarchical pores in the materials, pre-constructed mesoporous/ microporous structures are always used as precursors. There is a wide range of these materials, including hollow polymer nanotubes, 33 nanocomposites 116,117 and biomass aerogels (Fig. 7b).…”

Biobased Tannic Acid-Chitosan Composite Membranes as Reusable Adsorbents for Effective Enrichment of Phosphopeptides

“…The low abundance and dynamics of phosphopeptides make the enrichment of phosphopeptides an integral part of the phosphoproteomics analysis workflow . In recent years, based on typical enrichment strategies, such as immobilized metal ion affinity chromatography (IMAC), − metal oxide affinity chromatography (MOAC), − and immunoprecipitation, − some emerging functional enrichment materials were discovered, including dual-functional materials that could simultaneously enrich different types of post-translational modification (PTM) peptides, − dual-mode materials that integrating different enrichment strategies, , and reusable materials that pursue the concept of green chemistry. Among them, IMAC exhibits high selectivity and sensitivity and also possesses stronger specificity for multiphosphopeptides.…”

Titanium dioxide‐functionalized dendritic mesoporous silica nanoparticles for highly selective isolation of phosphoproteins

“…Dendritic mesoporous silica nanoparticles (DMSNs) are a new type of porous silica material with an open three-dimensional dendritic framework [32]. Benefiting from their features, for example, unique central-radial pore structure, large pore size, highly accessible inner surface, ease of modification, and excellent biocompatibility, DMSNs have been regarded as an excellent carrier platform for the loading of biomacromolecules [33][34][35][36]. For instance, Ti 4+ immobilized DMSNs exhibited excellent phosphopeptides enrichment performance, and the large pore sizes could successfully prevent the "shadow effect," facilitating efficient diffusion and release of phosphopeptides [37].…”