HHV-8 infection is specific for cell lines derived from primary effusion (body cavity-based) lymphomas

Lymphoma cell lines: in vitro models for the study of HHV-8+ primary effusion lymphomas (body cavity-based lymphomas)

“…Appropriately, all cell lines contain HHV-8 sequences but either lack or harbor EBV sequences. Previous studies showed that among a spectrum of some 240 human cell lines (comprising solid tumor-derived cell lines and hematopoetic leukemialymphoma cell lines, many of which were established from pleural effusion or ascites material) only PEL cell lines are HHV-8+ 23 (Table 1). These PEL cell lines have already played instrumental roles in the analysis of the biology of HHV-8.…”

Molecular profile of Epstein–Barr virus infection in HHV-8-positive primary effusion lymphoma

“…5,6 Recently, PEL has been recognized as a unique lymphoma entity based on its distinctive features which include presentation as lymphomatous effusion in the serous body cavities and consistent infection of the tumor clone by human herpesvirus type 8 (HHV-8). [1][2][3][4][5][6][7][8][9][10][11] In addition to HHV-8, PEL cells are also frequently co-infected by Epstein-Barr virus (EBV). [1][2][3][4][5][6][7][8][9][10]12 The role of viral infection in PEL pathogenesis is not fully understood.…”

“…[1][2][3][4][5][6][7][8][9][10][11] In addition to HHV-8, PEL cells are also frequently co-infected by Epstein-Barr virus (EBV). [1][2][3][4][5][6][7][8][9][10]12 The role of viral infection in PEL pathogenesis is not fully understood. On the one hand, the pathogenetic role of HHV-8 is suggested by the consistency of infection and by the expression of a subset of HHV-8 genes in PEL cells.…”

“…On the one hand, the pathogenetic role of HHV-8 is suggested by the consistency of infection and by the expression of a subset of HHV-8 genes in PEL cells. [1][2][3][4][5][6][7][8][9][10]13,14 On the other hand, the pathogenetic contribution of EBV is unclear. In fact, EBV infection occurs in 70% of PEL, is typically latent, and is generally monoclonal, suggesting that the virus infects the tumor cells since the early phases of clonal expansion.…”

The tyrosine kinase receptor Met and its ligand HGF are co-expressed and functionally active in HHV-8 positive primary effusion lymphoma

“…The PEL cell lines forming the basis of this study were HBL-6, BC-1, BC-2, CRO-AP/2, CRO-AP/3, CRO-AP/5, BCBL-1, BC-3 and BCP-1. [23][24][25][26][27][28][29][30] The detailed characterization of these cell lines has been reported previously. 23 31,32 Seven out of nine PEL specimens were derived from HIV-infected patients, whereas two PEL samples (BC-3 and BCP-1) were derived from HIV-negative individuals.…”