Hfq: A Multifaceted RNA Chaperone Involved in Virulence

Feliciano, Joana R.; Grilo, André; Guerreiro, Soraia I.; Sousa, Sílvia A.; Leitão, Jorge H.

doi:10.2217/fmb.15.128

Cited by 35 publications

(30 citation statements)

References 141 publications

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“…These factors include extracellular polysaccharide (EPS), extracellular enzymes (protease, endoglucanase and amylase) (Ryan et al ., ). In other pathogenic bacteria, Hfq has been shown to control virulence through modulation of the production of specific virulence factors (Chao and Vogel, ; Feliciano et al ., ). To clarify whether Hfq Xcc exhibits any influence on the production of key virulence factors, we compared EPS production and extracellular enzymes’ activity of the Δ hfq mutant with the wild‐type strain (see section on ‘Methods’ for details).…”

Section: Resultsmentioning

confidence: 97%

“…Similarly, Hfq has been reported to be essential for the virulence of Vibrio cholera and Salmonella typhimurium (Chao and Vogel, ). Currently, Hfq has been reported to be involved in the regulation of virulence in approximately two dozen human and animal bacterial pathogens (Chao and Vogel, ; Mansfield et al ., ; Wilms et al ., ; Zeng et al ., ; Feliciano et al ., ). Despite extensive work on this protein only a limited amount of work has been carried out examining the potential role of Hfq in the virulence regulation of plant pathogenic bacteria.…”

Section: Introductionmentioning

confidence: 97%

“…Later it was found that inactivation of hfq in E. coli exhibited broad, pleiotropic phenotypes affecting growth rate, cell morphology and tolerance of stress conditions (Tsui et al ., ). Since these experiments it is now acknowledged that Hfq orthologues are widespread and highly conserved in both Gram‐positive and Gram‐negative bacterial species (Sun et al ., ; Valentin‐Hansen et al ., ; Chao and Vogel, ; Sobrero and Valverde, ; Feliciano et al ., ; Updegrove et al ., ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The RNA chaperone Hfq is important for the virulence, motility and stress tolerance in the phytopathogen Xanthomonas campestris

Jie-ling

Tang

Liang

et al. 2018

Environ Microbiol Rep

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The RNA chaperone, Hfq, is known to play extensive roles in bacterial growth and development. More recently, it has been shown to be required for virulence in many human and animal bacterial pathogens. Despite these studies little is known about the role Hfq plays in phytopathogenic bacteria. In this study, we show Hfq is required for full virulence of the crucifer black rot pathogen Xanthomonas campestris pv. campestris (Xcc). We demonstrate that an Xcc hfq deletion strain is highly attenuated for virulence in Chinese radish and shows a severe defect in the production of virulence factors including extracellular enzymes and extracellular polysaccharide. Furthermore, the Xcc strain lacking Hfq had significantly reduced cell motility and stress tolerance. These findings suggest that Hfq is a key regulator of important aspects of virulence and adaptation of Xcc. Taken together, our findings are suggestive of a regulatory network placing Hfq at the centre of virulence gene expression control in Xcc.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The RNA chaperone Hfq is important for the virulence, motility and stress tolerance in the phytopathogen Xanthomonas campestris

Jie-ling

Tang

Liang

et al. 2018

Environ Microbiol Rep

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“…Several sRNAs have been found inactive in cells lacking Hfq protein, which has been described as a master regulator of gene expression in bacteria, essentially due to its ability to mediate the interaction of sRNAs with their mRNA targets, including those related to virulence in Gram-negative bacteria (Kim et al, 2015; Feliciano et al, 2016). The association between Hfq and virulence in E. coli was revealed by Kulesus et al (2008).…”

Section: Srnas Involved In the Virulence Of Human Pathogenic Vibrio Sppmentioning

confidence: 99%

Role of Non-coding Regulatory RNA in the Virulence of Human Pathogenic Vibrios

et al. 2017

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In recent decades, the identification of small non-coding RNAs in bacteria has revealed an important regulatory mechanism of gene expression involved in the response to environmental signals and to the control of virulence. In the family Vibrionaceae, which includes several human and animal pathogens, small non-coding RNAs (sRNAs) are closely related to important processes including metabolism, quorum sensing, virulence, and fitness. Studies conducted in silico and experiments using microarrays and high-throughput RNA sequencing have led to the discovery of an unexpected number of sRNAs in Vibrios. The present review discusses the most relevant reports regarding the mechanisms of action of sRNAs and their implications in the virulence of the main human pathogens in the family Vibrionaceae: Vibrio parahaemolyticus, V. vulnificus and V. cholerae.

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“…Therefore, Hfq provides RNA molecule with moored platform to promote the interaction of uncoded sRNA and target mRNA molecule (Mikulecky et al 2004;Schu et al 2015). Currently, more and more researches founded that Hfq and sRNAs has an essential effect on various physiological functions of many bacteria, such as growth features, environmental stress and virulence, when the Hfq is damaged, vibrio cholera, haemophilus bleeding and yersinia enterocolitica will be significantly attenuated (Lenz et al 2004;Hempei et al 2013; Kakoschke et al 2014;Feliciano et al 2016). …”

Section: Introductionmentioning

confidence: 99%

Modulation of Quorum-Regulatory sRNA on Various Physiological Functions and Virulence of Vibrio alginolyticus

Liu¹,

Yang²,

Zhang³

et al. 2017

Proceedings of the 2016 2nd International Conference on Materials Engineering and Information Technology Applications (MEITA 20

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Abstract. Vibrio alginolyticus is one of the important fish pathogen in China, bringing about serious economic loss to aquaculture. sRNA (Small non-coding RNA, sRNA) modulate the target genes on the post-transcriptional level with the aid of the chaperon protein, Hfq and play essential roles in the regulation of various physiological functions, like virulence. In this paper, a quorum regulatory sRNA, Qrr1, was screened and cloned. Besides, An in-frame deletion was made in qrr1 by allelic exchange, using the suicide plasmid. Meanwhile, the plasmid, pMMB206 was utilized to carry the contact qrr1 gene into the mutant strain Δqrr1 to generate the complemented strain, qrr1 + . The roles of this gene in different physiological functions of Vibrio alginolyticus were characterized. The results demonstrated that Qrr1 obviously promoted the growth of Vibrio alginolyticus during exponential and stationary stage. Moreover, the ability of mobility was almost absent, the biofilm formation and extracellular protease production were all impaired in the Δqrr1 mutant strain, contrast to the wild-type strain, which means Qrr1 participates in the virulence regulation as an important regulator in Vibrio alginolyticus.

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Hfq: A Multifaceted RNA Chaperone Involved in Virulence

Cited by 35 publications

References 141 publications

The RNA chaperone Hfq is important for the virulence, motility and stress tolerance in the phytopathogen Xanthomonas campestris

The RNA chaperone Hfq is important for the virulence, motility and stress tolerance in the phytopathogen Xanthomonas campestris

Role of Non-coding Regulatory RNA in the Virulence of Human Pathogenic Vibrios

Modulation of Quorum-Regulatory sRNA on Various Physiological Functions and Virulence of Vibrio alginolyticus

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