Role of Non-coding Regulatory RNA in the Virulence of Human Pathogenic Vibrios

Pérez-Reytor, Diliana; Plaza, Nicolás; Espejo, Romilio T.; Navarrete, Paola; Bastías, Roberto; García, Katherine

doi:10.3389/fmicb.2016.02160

Cited by 24 publications

(17 citation statements)

References 114 publications

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“…Additionally, several regulatory proteins involved in quorum sensing and biofilm formation, including Hfq, LuxS, and SmcR (Kim et al, 2013;Wen et al, 2016), were found to be acetylated. The role of these proteins in the regulation of virulence genes in genus Vibrio has also been widely reported (Waters et al, 2010;Pérez-Reytor et al, 2016), and our finding further shows the potential association between protein acetylation and the virulence of V. vulnificus.…”

Section: Functional Annotation and Enrichment Of Acetylated Proteins supporting

confidence: 83%

Genome- and Proteome-Wide Analysis of Lysine Acetylation in Vibrio vulnificus Vv180806 Reveals Its Regulatory Roles in Virulence and Antibiotic Resistance

et al. 2020

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Infection with Vibrio vulnificus is notorious for its atypical clinical manifestations and irreversible disease progression. Lysine acetylation is a conserved post-translational modification (PTM) that plays a critical regulatory role in diverse cellular processes. However, little is known about the role of lysine acetylation on the pathogenesis of V. vulnificus. Here, we report the complete genome sequence and a global profile for protein lysine acetylation of V. vulnificus Vv180806, a highly cefoxitin resistant strain isolated from a mortality case. The assembled genome comprised two circular chromosomes and one circular plasmid; it contained 4,770 protein-coding genes and 153 RNA genes. Phylogenetic analysis revealed genetic homology of this strain with other V. vulnificus strains from food sources. Of all the proteins in this strain, 1,924 (40.34%) were identified to be acetylated at 6,626 sites. The acetylated proteins were enriched in metabolic processes, binding functions, cytoplasm, and multiple central metabolic pathways. Moreover, the acetylation was found in most identified virulence factors of this strain, suggesting its potentially important role in bacterial virulence. Our work provides insights into the genomic and acetylomic features responsible for the virulence and antibiotic resistance of V. vulnificus, which will facilitate future investigations on the pathogenesis of this bacterium.

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Section: Functional Annotation and Enrichment Of Acetylated Proteins supporting

confidence: 83%

Genome- and Proteome-Wide Analysis of Lysine Acetylation in Vibrio vulnificus Vv180806 Reveals Its Regulatory Roles in Virulence and Antibiotic Resistance

et al. 2020

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“…The autoinducer-receptor systems in V. parahaemolyticus are likely similar to those in V. harveyi, based on phylogenetic analyses and signaling assays (10,14,16,42), whereas only the LuxPQ system that detects AI-2 has been identified in V. vulnificus (40). The number of Qrrs in different Vibrio species varies from one to five among vibrios, and they act either additively or redundantly (15,45,(52)(53)(54)(55)(56). Further, the Qrr regulon in V. harveyi (ϳ20 genes) does not appear to include the type VI secretion genes, which are regulated by the Qrrs in V. cholerae (50,57).…”

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confidence: 99%

Quorum Sensing Gene Regulation by LuxR/HapR Master Regulators in Vibrios

2017

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The coordination of group behaviors in bacteria is accomplished via the cell-cell signaling process called quorum sensing. Vibrios have historically been models for studying bacterial communication due to the diverse and remarkable behaviors controlled by quorum sensing in these bacteria, including bioluminescence, type III and type VI secretion, biofilm formation, and motility. Here, we discuss the Vibrio LuxR/HapR family of proteins, the master global transcription factors that direct downstream gene expression in response to changes in cell density. These proteins are structurally similar to TetR transcription factors but exhibit distinct biochemical and genetic features from TetR that determine their regulatory influence on the quorum sensing gene network. We review here the gene groups regulated by LuxR/ HapR and quorum sensing and explore the targets that are common and unique among Vibrio species.

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“…Small RNAs have been widely studied especially in the context of virulence regulation in pathogenic bacteria 31 , 37 , 38 , 55 . In Vibrionacea, small RNAs has been intensively studied in V. cholerae 56 . However, in other species, the role of small RNAs awaits further elucidation.…”

Section: Discussionmentioning

confidence: 99%

Quorum sensing and iron-dependent coordinated control of autoinducer-2 production via small RNA RyhB in Vibrio vulnificus

Lee

Wen

Park

et al. 2022

Sci Rep

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Roles for the non-coding small RNA RyhB in quorum-sensing and iron-dependent gene modulation in the human pathogen V. vulnificus were assessed in this study. Both the quorum sensing master regulator SmcR and the Fur-iron complex were observed to bind to the region upstream of the non-coding small RNA RyhB gene to repress expression, which suggests that RyhB is associated with both quorum-sensing and iron-dependent signaling in this pathogen. We found that expression of LuxS, which is responsible for the biosynthesis of autoinducer-2 (AI-2), was higher in wild type than in a ryhB-deletion isotype. RyhB binds directly to the 5′-UTR (untranslated region) of the luxS transcript to form a heteroduplex, which not only stabilizes luxS mRNA but also disrupts the secondary structure that normally obscures the translational start codon and thereby allows translation of LuxS to begin. The binding of RyhB to luxS mRNA requires the chaperone protein Hfq, which stabilizes RyhB. These results demonstrate that the small RNA RyhB is a key element associated with feedback control of AI-2 production, and that it inhibits quorum-sensing signaling in an iron-dependent manner. This study, taken together with previous studies, shows that iron availability and cell density signals are funneled to SmcR and RyhB, and that these regulators coordinate cognate signal pathways that result in the proper balance of protein expression in response to environmental conditions.

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Role of Non-coding Regulatory RNA in the Virulence of Human Pathogenic Vibrios

Cited by 24 publications

References 114 publications

Genome- and Proteome-Wide Analysis of Lysine Acetylation in Vibrio vulnificus Vv180806 Reveals Its Regulatory Roles in Virulence and Antibiotic Resistance

Genome- and Proteome-Wide Analysis of Lysine Acetylation in Vibrio vulnificus Vv180806 Reveals Its Regulatory Roles in Virulence and Antibiotic Resistance

Quorum Sensing Gene Regulation by LuxR/HapR Master Regulators in Vibrios

Quorum sensing and iron-dependent coordinated control of autoinducer-2 production via small RNA RyhB in Vibrio vulnificus

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