Hexane fraction from Brazilian <i>Morus nigra</i> leaves improved oral carbohydrate tolerance and inhibits α-amylase and α-glucosidase activities in diabetic mice

Silva, Dionísio Henrique Amaral da; Barbosa, Humberto de Moura; Beltrão, Raphaelle Lima de Almeida; Silva, Clarissa de França Oliveira; Moura, Celuane Alves; Castro, Rosane Nora; Almeida, Jackson Roberto Guedes da Silva; Gomes, Dayane Aparecida; Lira, Eduardo Carvalho

doi:10.1080/14786419.2020.1723087

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…The n-hexane component of Morus alba has also been demonstrated to lower blood glucose in mice. In a diabetic mouse model, the n-hexane extract of Morus alba was administered and found to delay the digestion and absorption of carbohydrates, reduce postprandial blood glucose values and inhibit the activity of α-glucosidase ( Silva et al, 2021a ). The extract was obtained by extracting mulberry leaves with ethanol and 48 rats were grouped and treated by gavage for 11 weeks, after which the islet β-cell function, insulin resistance index, fasting blood glucose, plasma insulin levels and the histomorphology of islets were measured.…”

Section: In Vitro and In Vivo Evaluation ...mentioning

confidence: 99%

“…Researchers used column chromatography to elute and separate the aqueous extract of mulberry leaves to obtain flavonoids including rutin, isoquercitrin, kaempferol-3-0-rutinoside and astragaloside, and used these compounds to conduct in vitro α-glucosidase inhibition experiments using microplate method, and found that these compounds The inhibition rates of these compounds were found to be dose-dependent for α-glucosidase,Their IC 50 values were 13.19 ± 1.10, 116.7 ± 1.17, 365.4 ± 1.05 and 15.82 ± 1.11 μM respectively ( Hong et al, 2013 ). In addition to the inhibition of α-glucosidase by extraction of the aqueous fraction, the flavonoids obtained from the extracted fraction of mulberry leaves with non-polar components were administered to diabetic mice, and after a period of treatment, the oral carbohydrate tolerance and blood glucose values of the diabetic mice were improved, and the activities of α-amylase and α-glucosidase were inhibited ( Silva et al, 2021a ). It has also been shown that the addition of flavonoid components (5,6,7-trihydroxyflavone sapogenins) to 1-DNJ-containing diets can have a synergistic effect by increasing the inhibition of α-glucosidase by 1-DNJ, probably due to the fact that flavonoid sapogenins can act as an inhibitor that can bind to the non-competitive position of maltosidase-glucoamylase, allowing 1-DNJ to interact with α-glucoamylase.…”

Section: Molecular Mechanism Of Hypoglycemia In Mulberry Leavesmentioning

confidence: 99%

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Evaluation of mulberry leaves’ hypoglycemic properties and hypoglycemic mechanisms

Chen

Gao

et al. 2023

Front. Pharmacol.

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The effectiveness of herbal medicine in treating diabetes has grown in recent years, but the precise mechanism by which it does so is still unclear to both medical professionals and diabetics. In traditional Chinese medicine, mulberry leaf is used to treat inflammation, colds, and antiviral illnesses. Mulberry leaves are one of the herbs with many medicinal applications, and as mulberry leaf study grows, there is mounting evidence that these leaves also have potent anti-diabetic properties. The direct role of mulberry leaf as a natural remedy in the treatment of diabetes has been proven in several studies and clinical trials. However, because mulberry leaf is a more potent remedy for diabetes, a deeper understanding of how it works is required. The bioactive compounds flavonoids, alkaloids, polysaccharides, polyphenols, volatile oils, sterols, amino acids, and a variety of inorganic trace elements and vitamins, among others, have been found to be abundant in mulberry leaves. Among these compounds, flavonoids, alkaloids, polysaccharides, and polyphenols have a stronger link to diabetes. Of course, trace minerals and vitamins also contribute to blood sugar regulation. Inhibiting alpha glucosidase activity in the intestine, regulating lipid metabolism in the body, protecting pancreatic -cells, lowering insulin resistance, accelerating glucose uptake by target tissues, and improving oxidative stress levels in the body are some of the main therapeutic properties mentioned above. These mechanisms can effectively regulate blood glucose levels. The therapeutic effects of the bioactive compounds found in mulberry leaves on diabetes mellitus and their associated molecular mechanisms are the main topics of this paper’s overview of the state of the art in mulberry leaf research for the treatment of diabetes mellitus.

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Section: In Vitro and In Vivo Evaluation ...mentioning

confidence: 99%

Section: Molecular Mechanism Of Hypoglycemia In Mulberry Leavesmentioning

confidence: 99%

Evaluation of mulberry leaves’ hypoglycemic properties and hypoglycemic mechanisms

Chen

Gao

et al. 2023

Front. Pharmacol.

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“…In this context, in Brazil, there are several medicinal plants that are used as herbal medicines by the rural and urban populations (Dutra et al, 2016;Pio et al, 2019). A single species can produce numerous chemical compounds with diverse pharmacological activities (Mendonça et al, 2019), including antibacterial (Pandini et al, 2018;Emre et al, 2020;Pacheco et al, 2020), anti-inflammatory (Ribeiro et al, 2018;Almohawes and Alruhaimi, 2020), antioxidant (Barth et al, 2018;Pontes et al, 2019;Vale et al, 2019), wound-healing properties (Ribeiro Neto et al, 2020), antihyperglycemic effect (Silva et al, 2020), and cardiovascular activity (Moreira et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical characterization, and antioxidant and antibacterial activities of the hydroethanolic extract of Anadenanthera peregrina stem bark

et al. 2022

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The Brazilian Cerrado biome consists of a great variety of endemic species with several bioactive compounds, and Anadenanthera peregrina (L.) Speg is a promising species. In this study, we aimed to perform phytochemical characterization and evaluate the antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli of the hydroethanolic extract of A. peregrina stem bark. The barks were collected in the Botanical Garden of Goiânia, Brazil. The hydroethanolic extract was obtained by percolation and subjected to physicochemical screening, total phenolic content estimation, high-performance liquid chromatography (HPLC) fingerprinting, and antioxidant (IC50 values were calculated for the 2,2-diphenyl-1-picrylhydrazyl assay - DPPH) and antibacterial activity determination. The pH of the extract was 5.21 and density was 0.956 g/cm3. The phytochemical screening indicated the presence of cardiac glycosides, organic acids, reducing sugars, hemolytic saponins, phenols, coumarins, condensed tannins, flavonoids, catechins, depsides, and depsidones derived from benzoquinones. The extract showed intense hemolytic activity. The total phenolic content was 6.40 g GAE 100 g-1. The HPLC fingerprinting analysis revealed the presence of gallic acid, catechin, and epicatechin. We confirmed the antioxidant activity of the extract. Furthermore, the extract did not inhibit the growth of E. coli colonies at any volume tested, but there were halos around S. aureus colonies at all three volumes tested. These results contribute to a better understanding of the chemical composition of A. peregrina stem bark and further support the medicinal applications of this species.

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“…The plant leaves constitute quercetin and chlorogenic acid (Zeni et al, 2017), and the fruit has keracyanin, kuromanin, cyanidin, cyanidin‐3‐glucoside (C3G), cyanidin‐3‐xyloside, and cyanidin‐3‐rutinoside (Suttisansanee et al, 2020; Yazdankhah et al, 2019). Other constituents are isoquercitrin, kaempferol‐3‐ O ‐rhamnoside (Silva et al, 2020), gallic acid, catechin, and rutin (Diab et al, 2020). Moreover, M. nigra fruit is a potential source of anthocyanins (Aybastier, 2021).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical characterization ofMorus nigrafruit ultrasound‐assisted ethanolic extract for its cardioprotective potential

Maqsood

Khan

Sharif

et al. 2022

Journal of Food Biochemistry

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The current work investigated the phytochemical profile of ultrasound‐assisted ethanolic extract of Morus nigra (M. nigra) fruit. FTIR analysis of M. nigra fruit extract revealed the presence of alcohols (O‐H), alkanes (C‐H stretch), alkenes (C=C), and alkynes (C≡C). The HPLC analysis quantified the quercetin, gallic acid, vanillic acid, chlorogenic acid, syringic acid, cinnamic acid, sinapic acid, and kaempferol. Furthermore, the cardioprotective activity of ethanolic extract of M. nigra fruit was investigated. Cholesterol supplementation (2%) in the daily diet and exposure to cigarette smoke (2 cigarettes twice a day) were to induce hypertension in rats. The experimental animals were categorized into four groups: G0 (negative control), G1 (positive control), G2 (standard drug), and G3 (M. nigra fruit). The fruit extract administration at 300 mg/kg BW/day orally for 2 months significantly (p < .001) enhanced the activities of serum and cardiac tissue antioxidants in hypertensive rats. Meanwhile, the fruit extract reduced the elevated serum lipid profile while significantly increasing the high‐density lipoproteins in G3 than G1 and G2. The increase in blood pressure, liver transaminases, and serum lactate dehydrogenase also reduced significantly in M. nigra fruit extract‐treated rats. Histopathological findings revealed mild normalization of cardiac myocytes with central nuclei, branching, and cross‐striations. Consequently, the M. nigra fruit extract exerted the cardioprotective potential via increasing the antioxidant enzymes and reducing the lipids, lactate dehydrogenase, liver transaminases, and blood pressure. The therapeutic potential of M. nigra fruit can be due to flavonols and phenolic acids. Practical applications The present work quantified the Morus nigra fruit phytochemicals and its significant role in reducing lipid markers and blood pressure and improving antioxidant status in rats fed a hypercholesterolemic diet and exposed to cigarette smoke. Conclusively, the inclusion of M. nigra fruit in daily diet could improve the cardiac health of the individuals. Furthermore, the therapeutic potential of M. nigra fruit and its isolated constituents in modulating the gene expression against cardiac problems can explore after clinical trials and standardization in higher animals.

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Hexane fraction from Brazilian Morus nigra leaves improved oral carbohydrate tolerance and inhibits α-amylase and α-glucosidase activities in diabetic mice

Cited by 6 publications

References 13 publications

Evaluation of mulberry leaves’ hypoglycemic properties and hypoglycemic mechanisms

Evaluation of mulberry leaves’ hypoglycemic properties and hypoglycemic mechanisms

Phytochemical characterization, and antioxidant and antibacterial activities of the hydroethanolic extract of Anadenanthera peregrina stem bark

Phytochemical characterization ofMorus nigrafruit ultrasound‐assisted ethanolic extract for its cardioprotective potential

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