1997
DOI: 10.1002/pro.5560060513
|View full text |Cite
|
Sign up to set email alerts
|

Hexafluoroacetone hydrate as a structure modifier in proteins: Characterization of a molten globule state of hen egg‐white lysozyme

Abstract: A molten globule-like state of hen egg-white lysozyme has been characterized in 25% aqueous hexafluoroacetone hydrate (HFA) by CD, fluorescence, NMR, and H/D exchange experiments. The far UV CD spectra of lysozyme in 25% HFA supports retention of native-like secondary structure while the loss of near UV CD bands are indicative of the overall collapse of the tertiary structure. The intermediate state in 25% HFA exhibits an enhanced a f f i n i t y towards the hydrophobic dye, ANS, and a native-like tryptophan f… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

3
23
0

Year Published

1997
1997
2019
2019

Publication Types

Select...
9

Relationship

2
7

Authors

Journals

citations
Cited by 36 publications
(26 citation statements)
references
References 62 publications
3
23
0
Order By: Relevance
“…The compact denatured state of b-lactoglobulin was reported not only in methanol but also in several types of organic solvents including ethanol, isopropanol, dimethylformamide, and dioxane~Uversky et al, 1997!. The compact denatured state in hexafluoroacetone hydrate was also reported for lysozymẽ Bhattacharjya & Balaram, 1997!. There results also indicate the general existence of the MG state in alcohol-water solvents.…”
Section: General Existence Of the Compact Denatured Conformations In supporting
confidence: 55%
“…The compact denatured state of b-lactoglobulin was reported not only in methanol but also in several types of organic solvents including ethanol, isopropanol, dimethylformamide, and dioxane~Uversky et al, 1997!. The compact denatured state in hexafluoroacetone hydrate was also reported for lysozymẽ Bhattacharjya & Balaram, 1997!. There results also indicate the general existence of the MG state in alcohol-water solvents.…”
Section: General Existence Of the Compact Denatured Conformations In supporting
confidence: 55%
“…(9,31,32) Hexafluoroacetone hydrate (HFA) is an amphiphilic molecule possessing a highly hydrophobic fluorocarbon face and a hydrophobic hydrogen bond donating hydrated face. Balaram and Bhattacharya (33) have observed a molten globule-like state of hen egg-white lysozyme in 0.25 mass fraction aqueous HFA. Figure 6 shows the far u.v.…”
Section: Resultsmentioning
confidence: 98%
“…Because of the dispersion turbidity, a quartz cuvette with optical path length of 0.20 cm was used. Data reported here refer to the k range 190-260 nm, where lysozyme shows typical absorbing bands centered at 208 and 222 nm, respectively [28]. The measuring temperature, 25.0°C, is controlled to 0.1°C by an external water circulation bath.…”
Section: Circular Dichroismmentioning
confidence: 99%