Heterotopic and orthotopic autologous chondrocyte implantation using a minipig chondral defect model

Lohan, Anke; Marzahn, Ulrike; Sayed, Karym El; Bock, C.; Haisch, Andreas; Kohl, Benjamin; Stoelzel, Katharina; John, Thilo; Ertel, Wolfgang; Schulze‐Tanzil, Gundula

doi:10.1016/j.aanat.2013.04.009

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…In a rabbit model (46) and minipig model (47) of articular repair, Lohan et al found inferior healing in constructs seeded with auricular chondrocytes. Our study could explain their observations.…”

Section: Discussionmentioning

confidence: 99%

Disparate response of articular- and auricular-derived chondrocytes to oxygen tension

Kean

Mera

Whitney

et al. 2016

Connective Tissue Research

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Purpose/Aim To determine the effect of reduced (5%) oxygen tension on chondrogenesis of auricular-derived chondrocytes. Currently, many cell and tissue culture experiments are performed at 20% oxygen with 5% carbon dioxide. Few cells in the body are subjected to this supra-physiological oxygen tension. Chondrocytes and their mesenchymal progenitors are widely reported to have greater chondrogenic expression when cultured at low, more physiological, oxygen tension (1–7%). Although generally accepted, there is still some controversy, and different culture methods, species, and outcome metrics cloud the field. These results are, however, articular chondrocyte biased and have not been reported for auricular-derived chondrocytes. Materials and Methods Auricular and articular chondrocytes were isolated from skeletally mature New Zealand White rabbits, expanded in culture and differentiated in high density cultures with serum free chondrogenic media. Cartilage tissue derived from aggregate cultures or from the tissue engineered sheets were assessed for biomechanical, glycosaminoglycan, collagen, collagen cross-links, and lysyl oxidase activity and expression. Results Our studies show increased proliferation rates for both auricular and articular chondrocytes at low (5%) O2 versus standard (20%) O2. In our scaffold free chondrogenic cultures, low O2 was found to increase articular chondrocyte accumulation of glycosaminoglycan, but not cross-linked type II collagen, or total collagen. Conversely, auricular chondrocytes accumulated less glycosaminoglycan, cross-linked type II collagen and total collagen under low oxygen tension. Conclusions This study highlights the dramatic difference in response to low O2 of chondrocytes isolated from different anatomical sites. Low O2 is beneficial for articular-derived chondrogenesis but detrimental for auricular-derived chondrogenesis.

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Section: Discussionmentioning

confidence: 99%

Disparate response of articular- and auricular-derived chondrocytes to oxygen tension

Kean

Mera

Whitney

et al. 2016

Connective Tissue Research

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“…Longer studies (6–12 months) are necessary to gain confidence in extent of success in the repair and regeneration of articular cartilage, including interface with adjacent cartilage and subchondral bone as well as the opposing articular surface (ASTM F2451–05 2010). A range of large animal models suitable for the assessment of cartilage repair have been investigated, including dogs (Engkvist 1979; Igarashi et al 2012; Breinan et al 2000), pigs (Hunziker et al 2001; Lohan et al 2013; Boopalan et al 2011; Klein et al 2009; Christensen et al 2015), sheep (Kon et al 2010a; Milano et al 2010; Erggelet et al 2009), goats (Getgood et al 2012; Jurgens et al 2013; Lu et al 2006; Wang et al 2007) and horses (Kon et al 2010b; Frisbie et al 2009; Hendrickson et al 1994). When using any large animal model it is important to determine where on the joint the defect should be created based on the biomechanics of the joint.…”

Section: Large Animal Modelsmentioning

confidence: 99%

The benefits and limitations of animal models for translational research in cartilage repair

et al. 2016

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Much research is currently ongoing into new therapies for cartilage defect repair with new biomaterials frequently appearing which purport to have significant regenerative capacity. These biomaterials may be classified as medical devices, and as such must undergo rigorous testing before they are implanted in humans. A large part of this testing involves in vitro trials and biomechanical testing. However, in order to bridge the gap between the lab and the clinic, in vivo preclinical trials are required, and usually demanded by regulatory approval bodies. This review examines the in vivo models in current use for cartilage defect repair testing and the relevance of each in the context of generated results and applicability to bringing the device to clinical practice. Some of the preclinical models currently used include murine, leporine, ovine, caprine, porcine, canine, and equine models. Each of these has advantages and disadvantages in terms of animal husbandry, cartilage thickness, joint biomechanics and ethical and licencing issues. This review will examine the strengths and weaknesses of the various animal models currently in use in preclinical studies of cartilage repair.

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“…19,47,51 When auricular chondrocytes were impregnated in the 3-dimensional polyglycolic acid, they demonstrated high chondrogenic potential and induced superior histological healing responses in vivo. 15,31,32 In our study, we showed that auricular chondrocytes could be induced by ECMs to gain phenotypes similar to articular chondrocytes in 2-dimensional culture, 3-dimensional culture, and in vivo transplantation. Our results confirmed the feasibility of switching the cellular phenotypes of chondrocytes exclusively via the microenvironment created by ECM biomaterials.…”

Section: Discussionmentioning

confidence: 64%

Facilitating In Vivo Articular Cartilage Repair by Tissue-Engineered Cartilage Grafts Produced From Auricular Chondrocytes

et al. 2017

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Heterotopic and orthotopic autologous chondrocyte implantation using a minipig chondral defect model

Cited by 8 publications

References 24 publications

Disparate response of articular- and auricular-derived chondrocytes to oxygen tension

Disparate response of articular- and auricular-derived chondrocytes to oxygen tension

The benefits and limitations of animal models for translational research in cartilage repair

Facilitating In Vivo Articular Cartilage Repair by Tissue-Engineered Cartilage Grafts Produced From Auricular Chondrocytes

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