Facilitating In Vivo Articular Cartilage Repair by Tissue-Engineered Cartilage Grafts Produced From Auricular Chondrocytes

Wong, Chin Chean; Chen, Chih-Hwa; Chiu, Li Hsuan; Tsuang, Yang Hwei; Bai, Meng Yi; Chung, Ren-Jei; Lin, Yuanhua; Hsieh, Fon Jou; Chen, You-Tzung; Yang, Tsung‐Lin

doi:10.1177/0363546517741306

Cited by 25 publications

(18 citation statements)

References 53 publications

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“…Instead, both Safranin O (associated or not with the fast green dye for the bone compartment) and Toluidine Blue were the staining methods most frequently chosen for proteoglycans as well as glycosaminoglycans content, in addition to the Alcian Blue staining method. Only five studies utilized the two classical techniques to visualize collagen fibers in histological section, picrosrius red [35] or Goldner's trichrome [16, 18, 21, 30]. Finally, for undecalcified samples, the Stevenel Blue/Van Gieson Pichrofucsin dye [29], thionine staining [27], and Toluidine Blue were used to evaluate the osteochondral compartment.…”

Section: Resultsmentioning

confidence: 99%

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Maglio

Brogini

Pagani

et al. 2019

BioMed Research International

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Osteochondral lesions (OCs) are typically of traumatic origins but are also caused by degenerative conditions, in primis osteoarthritis (OA). On the other side, OC lesions themselves, getting worse over time, can lead to OA, indicating that chondral and OC defects represent a risk factor for the onset of the pathology. Many animal models have been set up for years for the study of OC regeneration, being successfully employed to test different treatment strategies, from biomaterials and cells to physical and biological adjuvant therapies. These studies rely on a plethora of post-explant investigations ranging from histological and histomorphometric analyses to biomechanical ones. The present review aims to analyze the methods employed for the evaluation of OC treatments in each animal model by screening literature data within the last 10 years. According to the selected research criteria performed in two databases, 60 works were included. Data revealed that lapine (50% of studies) and ovine (23% of studies) models are predominant, and knee joints are the most used anatomical locations for creating OC defects. Analyses are mostly conducted on paraffin-embedded samples in order to perform histological/histomorphometric analyses by applying semiquantitative scoring systems and on fresh samples in order to perform biomechanical investigations by indentation tests on articular cartilage. Instead, a great heterogeneity is pointed out in terms of OC defect dimensions and animal's age. The choice of experimental times is generally adequate for the animal models adopted, although few studies adopt very long experimental times. Improvements in data reporting and in standardization of protocols would be desirable for a better comparison of results and for ethical reasons related to appropriate and successful animal experimentation.

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Section: Resultsmentioning

confidence: 99%

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Maglio

Brogini

Pagani

et al. 2019

BioMed Research International

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“…Cell plasticity is a key feature of stem/progenitor cells that is regulated by the environment of the surrounding tissue (MacLean et al 2017; Wong et al 2018). After transplantation, cellular differentiation and incorporation with host tissues are important to constitute functional histologic units.…”

Section: Discussionmentioning

confidence: 99%

Salispheres from Different Major Salivary Glands for Glandular Regeneration

et al. 2019

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Dysfunctional salivary glands (SGs) are a clinical challenge due to the lack of effective treatments. Cell therapy with stem/progenitor cells may improve this situation by providing promising therapeutic solutions. Therefore, exploring abundant cellular sources is important. Three major pairs of SGs are located in different anatomic regions: the parotid glands, the submandibular glands, and the sublingual glands. Although SG stem/progenitor cells can be isolated and cultivated from all major SGs as salispheres, the differences among SG origins remain unclear. In this study, salispheres were successfully isolated from all major SGs. The salispheres demonstrated unique cellular features that originated from their native tissues. The characteristic expression profiles and cellular features of SG stem cells were demonstrated in all salispheres. When they were transplanted into irradiated animals, the salispheres were all capable of improving the saliva secretion that was disrupted by irradiation. Typical histologic structures could be observed in most parts of the treated glands, and the fibrotic environments of irradiated submandibular glands were remodeled by all salispheres regardless of origins. This study characterized the cellular features and in vivo effects of salispheres that were derived from different anatomic origins. The results suggest the possibility of functional redundancy among distinct pairs of major SGs, which is useful for the design of cell therapy to treat dysfunctional glandular organs.

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“…Collagen hydrogels reduce the risk of immune rejection by stimulating the ECM formation, reduce immunogenicity of exogenous cells that are seeded in engineered hydrogel constructs and promote cartilage regeneration [ 99 ]. In addition, Wong et al found that type II collagen was capable of converting auricular chondrocytes into articular cartilage after dedifferentiation by monolayer passaging [ 100 ]. By using type I collagen as scaffold combined with stromal cell-derived factors-1 (SDF-1) as growth factor, Zhang et al successfully induced the migration and adhesion of C-MSCs and SM-MSCs and promoted the self-repair of partial thickness defects [ 30 ].…”

Section: Current State-of-art Of Hydrogels Designed For Cartilage Regmentioning

confidence: 99%

Advanced hydrogels for the repair of cartilage defects and regeneration

Wei

Yao

et al. 2021

Bioactive Materials

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176

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Cartilage defects are one of the most common symptoms of osteoarthritis (OA), a degenerative disease that affects millions of people world-wide and places a significant socio-economic burden on society. Hydrogels, which are a class of biomaterials that are elastic, and display smooth surfaces while exhibiting high water content, are promising candidates for cartilage regeneration. In recent years, various kinds of hydrogels have been developed and applied for the repair of cartilage defects in vitro or in vivo , some of which are hopeful to enter clinical trials. In this review, recent research findings and developments of hydrogels for cartilage defects repair are summarized. We discuss the principle of cartilage regeneration, and outline the requirements that have to be fulfilled for the deployment of hydrogels for medical applications. We also highlight the development of advanced hydrogels with tailored properties for different kinds of cartilage defects to meet the requirements of cartilage tissue engineering and precision medicine.

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Facilitating In Vivo Articular Cartilage Repair by Tissue-Engineered Cartilage Grafts Produced From Auricular Chondrocytes

Abstract: This study proposes a feasible methodology to convert heterotopic auricular chondrocytes for articular cartilage repair, which may serve as potential alternative sources for cartilage repair.

Cited by 25 publications

References 53 publications

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Current Trends in the Evaluation of Osteochondral Lesion Treatments: Histology, Histomorphometry, and Biomechanics in Preclinical Models

Salispheres from Different Major Salivary Glands for Glandular Regeneration

Advanced hydrogels for the repair of cartilage defects and regeneration

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