Advanced hydrogels for the repair of cartilage defects and regeneration

Wei, Wei; Ma, Yihua; Yao, Xudong; Zhou, Wenyan; Wang, Xiaozhao; Li, Chenglin; Lin, Junxin; He, Qun; Leptihn, Sebastian; Ouyang, Hongwei

doi:10.1016/j.bioactmat.2020.09.030

Cited by 214 publications

(154 citation statements)

References 168 publications

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“…biomaterials have been employed for engineering cartilage [2], each having its own advantages and disadvantages. Because hydrogels mimic cartilage tissue-like features, they are considered the frontline among scaffolds to treat cartilage defects [3].…”

Section: Introductionmentioning

confidence: 99%

Enhanced expression of hyaluronic acid in osteoarthritis-affected knee-cartilage chondrocytes during three-dimensional in vitro culture in a hyaluronic-acid-retaining polymer scaffold

Katoh

Yoshioka²,

Senthilkumar³

et al. 2021

The Knee

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Background: Chondrocyte transplantation to address cartilage damage is an established solution. Because hyaluronic acid (HA) is an essential component for homeostasis of the cartilage, in order to arrive at methodologies to utilize its advantages in cell-based therapies, we compared the HA retention capability of a thermoreversible gelation polymer scaffold-based environment (3D-TGP) with conventional in vitro cell culture methodologies. Methods: Chondrocytes derived from osteoarthritis-affected knee joint cartilage of elderly patients were used and accomplished in three phases. In Phase I, the levels of HA secreted by chondrocytes were measured in culture supernatant. In Phase II, retention capacity of externally added HA was quantified indirectly by measuring the HA released in culture supernatant, and in Phase III, the expression of CD44 on cells was analysed by immunohistochemistry. Results: In Phase I, the average HA in the 3D supernatant was 3% that of 2D. In phase II, 80% of externally added HA was detected in the 2D on day 7, while in 3D-TGP, only 0.1% was released until day 21. In Phase III, 2D yielded individual cells that started degenerating from the third week; in 3D-TGP cells grew for a longer duration, formed a tissue-like architecture with extracellular matrix with significantly intense staining of CD44 than 2D. Conclusion:The capability of the 3D-TGP culture environment to retain HA and support chondrocytes to grow with a tissue-like architecture expressing higher HA content is considered advantageous as it serves as an in vitro culture platform that enables tissue engineering of cartilage tissue with native hyaline phenotype and higher HA expression. The in vitro environment being conducive, based on this data, we also recommend that the TGP be tried as an encapsulation material in clinical studies of chondrocyte implantation for optimal clinical outcome.

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Section: Introductionmentioning

confidence: 99%

Enhanced expression of hyaluronic acid in osteoarthritis-affected knee-cartilage chondrocytes during three-dimensional in vitro culture in a hyaluronic-acid-retaining polymer scaffold

Katoh

Yoshioka²,

Senthilkumar³

et al. 2021

The Knee

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“…Various types of PLL-based nanomaterials have been investigated for soft tissue regeneration and reconstruction. Hydrogels are characterized regarding their structural similarity to natural ECMs, high water content, porous frameworks for cell proliferation and transplantation, ability to match irregular defects, among other properties , which make them attractive as scaffolds in cartilage and other soft tissue engineering [ 325 , 326 ]. Interestingly, the employment of PLL could not only enable the influence of swelling and degradation behavior of hydrogels but also enhance the cell absorption and proliferation because of its pendent primary cationic amine groups.…”

Section: Biomedical Applications Of Pll-based Polymersmentioning

confidence: 99%

Poly(α-l-lysine)-based nanomaterials for versatile biomedical applications: Current advances and perspectives

Zheng

Pan

Zhang

et al. 2021

Bioactive Materials

109

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Poly(α- l -lysine) (PLL) is a class of water-soluble, cationic biopolymer composed of α- l -lysine structural units. The previous decade witnessed tremendous progress in the synthesis and biomedical applications of PLL and its composites. PLL-based polymers and copolymers, till date, have been extensively explored in the contexts such as antibacterial agents, gene/drug/protein delivery systems, bio-sensing, bio-imaging, and tissue engineering. This review aims to summarize the recent advances in PLL-based nanomaterials in these biomedical fields over the last decade. The review first describes the synthesis of PLL and its derivatives, followed by the main text of their recent biomedical applications and translational studies. Finally, the challenges and perspectives of PLL-based nanomaterials in biomedical fields are addressed.

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“…In a healthy joint, the synovial fluid consists of different biomacromolecules, which act as efficient lubricant participating in the boundary lubrication of the joint at physiological pressures [ 16 , 17 ]. The biomacromolecules are also the important constituents for articular cartilage.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic injectable hydrogel microspheres with enhanced lubrication and controllable drug release for the treatment of osteoarthritis

Han

Yang

Zhao

et al. 2021

Bioactive Materials

135

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The occurrence of osteoarthritis (OA) is highly associated with the reduced lubrication property of the joint, where a progressive and irreversible damage of the articular cartilage and consecutive inflammatory response dominate the mechanism. In this study, bioinspired by the super-lubrication property of cartilage and catecholamine chemistry of mussel, we successfully developed injectable hydrogel microspheres with enhanced lubrication and controllable drug release for OA treatment. Particularly, the lubricating microspheres (GelMA@DMA-MPC) were fabricated by dip coating a self-adhesive polymer (DMA-MPC, synthesized by free radical copolymerization) on superficial surface of photo-crosslinked methacrylate gelatin hydrogel microspheres (GelMA, prepared via microfluidic technology), and encapsulated with an anti-inflammatory drug of diclofenac sodium (DS) to achieve the dual-functional performance. The tribological test and drug release test showed the enhanced lubrication and sustained drug release of the GelMA@DMA-MPC microspheres. In addition, the functionalized microspheres were intra-articularly injected into the rat knee joint with an OA model, and the biological tests including qRT-PCR, immunofluorescence staining assay, X-ray radiography and histological staining assay all revealed that the biocompatible microspheres provided significant therapeutic effect against the development of OA. In summary, the injectable hydrogel microspheres developed herein greatly improved lubrication and achieved sustained local drug release, therefore representing a facile and promising technique for the treatment of OA.

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Advanced hydrogels for the repair of cartilage defects and regeneration

Cited by 214 publications

References 168 publications

Enhanced expression of hyaluronic acid in osteoarthritis-affected knee-cartilage chondrocytes during three-dimensional in vitro culture in a hyaluronic-acid-retaining polymer scaffold

Enhanced expression of hyaluronic acid in osteoarthritis-affected knee-cartilage chondrocytes during three-dimensional in vitro culture in a hyaluronic-acid-retaining polymer scaffold

Poly(α-l-lysine)-based nanomaterials for versatile biomedical applications: Current advances and perspectives

Biomimetic injectable hydrogel microspheres with enhanced lubrication and controllable drug release for the treatment of osteoarthritis

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