Heterosubtypic protective immunity against influenza A virus induced by fusion peptide of the hemagglutinin in comparison to ectodomain of M2 protein

Staneková, Zuzana; Király, J.; Stropkovská, A.; Mikušková, T; Mucha, V; Kostolanský, F; Varečková, E

doi:10.4149/av_2011_01_61

Cited by 43 publications

(48 citation statements)

References 20 publications

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“…HA2 gp is a relatively conserved protein, and some HA2 epitopes are even shared among HA subtypes (Kostolanský et al, 2002;Varečková et al, 2008). HA2-specific antibodies recognizing HA stem were shown to be protective (Gocník et al, 2007(Gocník et al, , 2008Prabhu et al, 2009;Sui et al, 2009;Wang et al, 2010;Staneková et al, 2011;Janulíková et al, 2012), though they do not mediate virus neutralization by blocking the receptor-binding site on HA. We therefore suggest that anti-stem HA antibodies could help individuals exposed to dangerous avian IAV to overcome the infection in a subclinical manner.…”

Section: Discussionmentioning

confidence: 99%

Virus-neutralizing antibody response of mice to consecutive infection with human and avian influenza A viruses

Janulíková¹,

Stropkovská²,

Bobišová³

et al. 2015

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Summary. -In this work we simulated in a mouse model a naturally occurring situation of humans, who overcame an infection with epidemic strains of influenza A, and were subsequently exposed to avian influenza A viruses (IAV). The antibody response to avian IAV in mice previously infected with human IAV was analyzed. We used two avian IAV (A/Duck/Czechoslovakia/1956 (H4N6) and the attenuated virus rA/Viet Nam/1203-2004 (H5N1)) as well as two human IAV isolates (virus A/Mississippi/1/1985 (H3N2) of medium virulence and A/Puerto Rico/8/1934 (H1N1) of high virulence). Two repeated doses of IAV of H4 or of H5 virus elicited virus-specific neutralizing antibodies in mice. Exposure of animals previously infected with human IAV (of H3 or H1 subtype) to IAV of H4 subtype led to the production of antibodies neutralizing H4 virus in a level comparable with the level of antibodies against the human IAV used for primary infection. In contrast, no measurable levels of virus-neutralizing (VN) antibodies specific to H5 virus were detected in mice infected with H5 virus following a previous infection with human IAV. In both cases the secondary infection with avian IAV led to a significant increase of the titer of VN antibodies specific to the corresponding human virus used for primary infection. Moreover, cross-reactive HA2-specific antibodies were also induced by sequential infection. By virtue of these results we suggest that the differences in the ability of avian IAV to induce specific antibodies inhibiting virus replication after previous infection of mice with human viruses can have an impact on the interspecies transmission and spread of avian IAV in the human population.Keywords: avian influenza A viruses; sequential influenza A infection; antibody response; virus-neutralizing antibodies; HA2-specific antibodies; original antigenic sin * Corresponding author. E-mail: viruevar@savba.sk; phone: +421-2-59302427. Abbreviations: HA = hemagglutinin; IAV = influenza A virus; MAb = monoclonal antibody; OAS = original antigenic sin; VN = virus-neutralizing

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Section: Discussionmentioning

confidence: 99%

Virus-neutralizing antibody response of mice to consecutive infection with human and avian influenza A viruses

Janulíková¹,

Stropkovská²,

Bobišová³

et al. 2015

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“…The course of infection in mice immunized with FP and challenged with homologous influenza virus our previous data showed that immunization of mice with three doses of FP conjugated to klh significantly protected them against a lethal infection with 1 lD 50 of homologous virus A/Miss (Staneková et al, 2011). here, even though the infective dose was increased to 2 lD 50 , the protection induced by immunization was still observable.…”

Section: Cross-protective Effect Of Immunization With Fp On Lethal Inmentioning

confidence: 99%

“…Many studies have been focused on the M2 protein (okuda et al, 2001;Fan et al, 2004;liu et al, 2004;DeFilette et al, 2006;hoelscher et al, 2006;DeFilette et al, 2008a,b;huleatt et al, 2008;Zhang et al, 2009;li et al, 2011;Park et al, 2011;Staneková et al, 2011), but recently also the conserved part of hA, hA2 gp, was used as an inductor of cross-protective immunity (okuno et al, 1993;horváth et al, 1998;Gocník et al, 2008;Sui et al, 2009;Bommakanti et al, 2010;Steel et al, 2010;Wang et al, 2010;Staneková et al, 2011). The latter was the subject of our interest for several years.…”

Section: Introductionmentioning

confidence: 99%

Two distinct regions of HA2 glycopolypeptide of influenza virus hemagglutinin elicit cross-protective immunity against influenza

Janulíková¹,

Staneková²,

Mucha³

et al. 2012

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Summary. -currently, a new trend in development of vaccines against influenza with broader spectrum of efficacy is focused on conserved antigens of influenza virus. The hA2 glycopolypeptide (hA2 gp) is one of conserved antigens, potentially suitable as immunogens inducing cross-protection against influenza. We selected two distinct domains of hA2 gp originating from influenza A virus (IAv) of h3 subtype for induction of antiviral immune response: the ectodomain (ehA2) comprising aa 23-185 and the fusion peptide (FP) comprising n-terminal aa 1-38. BAlB/c mice were immunized with three doses of ehA2 and FP, respectively, and subsequently challenged with 2 lD 50 of IAv of homologous (h3) or heterologous (h7) hA subtype. Both peptides induced significant antibody response and protected mice against the lethal infection. The most efficient protection was achieved with ehA2 against homologous virus.Keywords: influenza A virus; cross-protection; hA2 glycopolypeptide; hA2 ectodomain; fusion peptide; mice; vaccine * corresponding author. e-mail: viruevar@savba.sk; phone: +4212-59302427. Abbreviations: A/chicken/Germany/34 (h7n1) virus, rostock strain; A/Miss = A/Mississippi/1/85 (h3n2) virus; A/rostock = avian hA = hemagglutinin; ehA2 = ectodomain of hA2 gp; FA = Freund΄s adjuvant; FP = fusion peptide; hA0 = hA precursor; hA1 = hA1 glycopolypeptide; hA2 = hA2 glycopolypeptide; IAv(s) = influenza A virus(es); MAb = monoclonal antibody; vrnA = viral rnA; p.i. = post infection

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“…Peptide vaccination is a key role of combining a good desirable immune response and a minimal immunological side effect. There are many peptide vaccines under development, such as vaccine for human immunodeficiency virus (HIV), hepatitis C virus (HCV), malaria, foot and mouth disease, swine fever, influenza, anthrax, human papilloma virus (HPV), therapeutic anti-cancer vaccines, pancreatic cancer, melanoma, non-small cell lung cancer, advanced hepatocellular carcinoma, cutaneous T-cell lymphoma and B-Cell chronic lymphocytic leukaemia [54][55][56][57][58][59][60][61][62][63][64][65][66][67].…”

Section: Discussionmentioning

confidence: 99%

Multi Epitope Peptide Vaccine Prediction against Sudan Ebola Virus Using Immuno-Informatics Approaches

Abu-haraz¹,

Abd-elrahman²,

Ibrahim³

et al. 2017

Adv Tech Biol Med

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Sudan Ebola virus is single stranded negative sense RNA genome belonging to Filovirus Filoviridae family that causes hemorrhagic fever. There is no treatment or vaccine for it, thus the aim of this study is to design a peptide vaccine using immuoinformatics approaches to analyse the glycoprotein of the all strain of SUDV, to determine the conserved region which is further studied to predict all possible epitopes that can be used as a peptide vaccine. A total of 21 Sudan Ebola virus glycoprotein retrieved from NCBI database were aligned to determine the conservancy and to predict the epitopes using IEDB analysis resource. Three epitopes predicted as a peptide vaccine for B cell (PPPPDGVR, ETFLQSPP, LQSPPIRE). For T cell four epitopes showed high affinity to MHC class I (FLYDRLAST, IIIAIIALL, MHNQNALVC and RTYTILNRK) and high coverage against Sudan and the whole world population. Also in MHC class II, Four epitopes that interact with most frequent MHC class II alleles (FAEGVIAFL, FLRATTELR, FLYDRLAST and FVWVIILFQ) with high coverage against Sudan and the whole world population. We recommend in vivo and in vitro study to prove the effectiveness of these predicted epitopes as a peptide vaccine.

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Heterosubtypic protective immunity against influenza A virus induced by fusion peptide of the hemagglutinin in comparison to ectodomain of M2 protein

Cited by 43 publications

References 20 publications

Virus-neutralizing antibody response of mice to consecutive infection with human and avian influenza A viruses

Virus-neutralizing antibody response of mice to consecutive infection with human and avian influenza A viruses

Two distinct regions of HA2 glycopolypeptide of influenza virus hemagglutinin elicit cross-protective immunity against influenza

Multi Epitope Peptide Vaccine Prediction against Sudan Ebola Virus Using Immuno-Informatics Approaches

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