Heterosis breeding in chilli pepper by using cytoplasmic male sterile lines for high-yield production with special reference to seed and bioactive compound content under temperature stress regimes

Meena, Om Prakash; Dhaliwal, M. S.; Jindal, S. K.

doi:10.1016/j.scienta.2019.109036

Cited by 13 publications

(21 citation statements)

References 86 publications

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“…The magnitude of MS attributed to the genotypes was much higher than the MS due to environments and the G × E interaction effects for all the traits. Earlier research study in which fruit traits were estimated have showed significant genetic variations among the progenies [10,[30][31][32]. Data's are mean sums of squares; *significant at p = 0.05; ns: non-significant at 5%level of significance…”

Section: Analysis Of Variance For Experimental Designmentioning

confidence: 99%

“…One of the most effective tools at the breeder's disposal is appraisal of the heterotic effect of F1 hybrid combination. The heterosis, i.e., hybrid vigor, measures the phenotypic superiority of F1 hybrids over their parents, and it is widely used for traits such as plant growth, quality fruit production, fertility, and other adaptive traits like resistance to abiotic and biotic stresses [10]. Heterosis of bell pepper has high utilization values.…”

Section: Introductionmentioning

confidence: 99%

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Exploitation of Heterosis among Phenotypically Diverse Capsicum Parents for Important Fruit Traits

Rani

Jindal

Meena

2021

Braz. arch. biol. technol.

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One nuclear male sterile (NMS) line of chilli pepper and eight elite inbred lines of bell pepper were evaluated in a line × tester mating design to project heterotic F1's through studying plant growth and yield performance of developed hybrids in relation to phenotypic distance between their parental lines. Hybrids and their parental lines were grown and evaluated under poly-net house conditions at the Punjab Agricultural University, Ludhiana, India during the years 2017-2019. Pooled over the year, mean squares (MS) due to genotypes was recorded significant (p=0.05) for all the traits studied, suggesting that there existed significant differences among the genotypic means that included nine parental lines and their eight F1 hybrids. The MS due to the G × Y interaction effects were also significantly different for plant growth and fruit traits, indicating differential response of each genotype in the year. On the basis of heterotic performance, the hybrids namely MS-12 × PAU SM-31 had positive significant mid-parent heterosis (MPH) for total fruit yield plant -1 (96.68**), number of fruits plant -1 (51.98**), pericarp thickness (47.91**), fruit length (25.77**), and plant height (63.35**). Apart from this cross-combination, the hybrids MS-12 × PAU SM-3 and MS-12 × PAU SM-9 also depicted MPH for total fruit yield plant -1 (71.22** and 50.64**), fruit length (41.98** and 68.09**) and number of fruits plant -1 (27.60** and 44.73**). Based on better parent heterosis (BPH), the hybrid MS-12 × PAU SM-HIGHLIGHTS  The effect of phenotypic distance between parental lines on the yield of C. annuum var. annuum × C. annuum var. grossum F1 hybrids was assessed. Simultaneously, the target of the research was transferring of the GMS ms10 gene from hot pepper to heat tolerant bell pepper inbreds. Over the BP, the hybrid MS-12 × PAU SM-31 was found to be superior in respect of total fruit yield plant -1 and pericarp thickness. 2 Rani, M.; et al.

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Section: Analysis Of Variance For Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploitation of Heterosis among Phenotypically Diverse Capsicum Parents for Important Fruit Traits

Rani

Jindal

Meena

2021

Braz. arch. biol. technol.

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“…C. annuum has a variety of bioactive compounds including capsaicin which showed great pharmaceutical and antimicrobial benefit. 6 , 7 The capsaicin in C. annuum showed therapeutic ability such as anticancer agent, antiobesity, cardiovascular effect, dermatological effect, and neuropathic pain relief. 4 C. annuum is native to Mexico, Peru, and other countries in the tropical region of Central and South America.…”

Section: Introductionmentioning

confidence: 99%

Integration of mRNA and miRNA profiling reveals the heterosis of three hybrid combinations of Capsicum annuum varieties

et al. 2021

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Capsicum annuum is also known as chili which is one of the most important vegetable crops grown in the world. Breeding new varieties with heterosis could improve the quality of pepper, increase yield, growth potential, disease resistance, adaptability, and seed viability. To investigate the heterosis among three cross combinations of different parents, the mRNA-miRNA integrated analysis was performed. A total number of 22,659,009 to 36,423,818 clean data were generated from mRNA-seq with 81 libraries, and the unique mapped reads were from 35,495,567 (86.81%) to 46,466,622 (88.95%). The plant-hormone signal transduction pathway (40 genes) was detected with a higher DEG number. The SAUR32L, GID1, PYR1, EIN2. ERF1, PR1, JAR1-like, IAA from this pathway play a key role in plant development. From the miRNA-seq, the number of clean reads was ranging from 12,132,221 to 25,632,680. A total of 220 miRNAs were predicted in this study, and all of them were identified as novel miRNA. The top three candidate KEGG pathways of miRNA were ribosome signaling pathway (13 miRNAs), spliceosome pathway (13 miRNAs), and plant hormone signal transduction pathways (10 miRNAs). With the mRNA and miRNA integrated analysis, we found some key genes were regulated by some miRNAs. Among them, the scarecrowlike 6 protein can be up or down regulated by mir8, mir120, mir184, mir_214, mir125, and mir130. The function of Della protein was regulated by mir24, mir74, mir94, mir139, and mir190. This study contributes to understanding how heterosis regulates the traits, such as crop production, fruit weight, and fruit length.

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“…Incidentally, the use of a male sterile (MS) line, which does not need emasculation during hybrid F 1 production, is a best solution for overcoming this problem (Meena et al 2020b). Both the genetic male sterility (GMS) and the nuclear-cytoplasmic male sterility (C-GMS or CMS) have been reported and utilized for hybrid development in Capsicum (Dhaliwal and Jindal 2014;Meena 2017).…”

Section: Introductionmentioning

confidence: 99%

Recurrent parent phenome recovery analysis in six different populations with the introgression of genetic male sterile gene ms10 from hot pepper to heat tolerant bell pepper

Rani

Jindal

Meena

2021

Preprint

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Backcrossing is one of the most commonly used breeding methods to transfer target trait(s) into a bell pepper inbreds. We attempted for the first time to transfer genetic male sterile (GMS) ms10 gene from the donor parent MS-12, popular GMS line in India, into the genetic background of six heat tolerant bell pepper inbreds, in restricted generations through the marker-assisted selection (MAS) and backcross breeding method, to develop new GMS lines in bell pepper. In this project, the recurrent parent phenome (RPP) recovery was analyzed in each generations of backcrossing using economic phenotypic markers. Phenotypic background analysis revealed that the extent of RPP recovery ranged from 43.59% (MSSM-1) to 66.26% (MSSM-21) and 70.89% (MSSM-17) to 92.09% (MSSM-21) for fruit weight, from 19.0% (MSSM-21) to 55.14% (MSSM-3) and 61.0% (MSSM-21) to 98.75% (MSSM-1) for number of lobes fruit− 1, from 55.77% (MSSM-17) to 94.25% (MSSM-2) and 69.83% (MSSM-17) to 98.26% (MSSM-3) for total fruit yield plant− 1 in BC1F1 and BC2F1 populations, respectively. Based on a paired 't' test analysis in BC2F1 population, out of six, the MSSM-1 and MSSM-2 generation was shown to be non-significant for all 19 studied traits followed by MSSM-3 (18), indicating a successful recovery of the recipient genome through marker-assisted backcross breeding after successful introgression of the ms10 gene. The GMS lines developed from this project will be utilized in bell pepper breeding programs aimed to identify the best cross-combination for their cultivation under north-Indian plains.

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Heterosis breeding in chilli pepper by using cytoplasmic male sterile lines for high-yield production with special reference to seed and bioactive compound content under temperature stress regimes

Cited by 13 publications

References 86 publications

Exploitation of Heterosis among Phenotypically Diverse Capsicum Parents for Important Fruit Traits

Exploitation of Heterosis among Phenotypically Diverse Capsicum Parents for Important Fruit Traits

Integration of mRNA and miRNA profiling reveals the heterosis of three hybrid combinations of Capsicum annuum varieties

Recurrent parent phenome recovery analysis in six different populations with the introgression of genetic male sterile gene ms10 from hot pepper to heat tolerant bell pepper

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