Heterologous ovum penetration by human spermatozoa

Tyler, J. P. P.; Pryor, J. P.; Collins, W. P.

doi:10.1530/jrf.0.0630499

Cited by 57 publications

(27 citation statements)

References 13 publications

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“…In the donor group the mean penetration rate of 53% coincided with the results of similar groups in different studies where the mean penetration rates in donor and fertile men were 56.3% [14], 54% [6], 55.9% [16], and 59.7% [15]. The mean penetration obtained in this study is low compared with some studies: 81% [19], 69% [ l o ] , and 66% [7].…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Hamdi

Ibrahim

Girgis

et al. 1985

Archives of Andrology

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The zona-free hamster egg penetration test was performed on semen samples from 21 fertile donors, 57 infertile patients with normal semen parameters, and 63 infertile patients with subnormal spermiogram, as well as on 19 frozen semen samples.In the donor group 100% of the samples gave a positive range of penetration (PRP) of 20-96% with a mean penetration rate (MPR) of 53%, while 84% of the frozen samples showed a PRP of 18-44% and an MPR of 31%.Although 74% of the samples from infertile patients with normal spermiograms gave a PRP of 11-9270 and an MPR of 34%, only 46% of the semen samples from the infertile patients with subnormal spermiogram showed a PRP of 21-100% but an MRP of 43%.

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Section: Discussionsupporting

confidence: 86%

“…The range of penetration in this group (11-92%) is also different from that of other investigators: 38-81% [17] and 0 4 . 7 % [15].…”

Section: Discussionmentioning

confidence: 99%

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Hamdi

Ibrahim

Girgis

et al. 1985

Archives of Andrology

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“…Although some investigators have found positive correlations between these results, others have failed to do so [5,10,13,19,20,22,261. In the present study the fertile and the infertile subjects differed in both the conventional semen parameters, sperm density and motility, as well as in the in vitro penetrations of hamster ova and bovine cervical mucus.…”

Section: Dlscussionmentioning

confidence: 99%

Relationship Between the Sperm Penetration Assay and Other Tests of Sperm Function

Fredericks

Abdel-latif

Abdel-Aal

et al. 1987

Archives of Andrology

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Semen from 88 men of infertile couples and 33 fertile donors differed in seminal fluid analysis (sperm density and motility) (SFA) as well as in the penetration of hamster ova (SPA) and bovine cervical mucus (MPT). In the fertile group, significantly more subjects had adequate SFA, SPA, or MPT results than in the infertile group. When the two groups were subdivided into those with normal or those with abnormal SFA, no differences were noted in SPA, MPT, or postcoital test (PCT) scores. The SFA parameter most consistently reflected in the results of the SPA, MPT, and PCT was sperm density. This was most evident when the SFA was poor. The worst prognosticator of fertility was the SFA, with 30% of the fertile donors having an abnormal SFA. The worst prognosticator of infertility was the MPT, with 79% of the patients penetrating in the fertile range. The SPA was a significantly better predictor than either the SFA or MPT. SPA and MPT results were positively correlated only in the overall infertile group. The SPA, MPT, and PCT measure sperm qualities distinct from those revealed by the SFA, and from each other, and in combination provide the best assessment of fertility.

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“…Subject C Within subject variability T h e variation in penetration rates between six different semen samples was studied for 3 individuals and is shown in Table 1 (Tyler et al 1981).…”

Section: Subject Bmentioning

confidence: 99%

“…The results obtained with 15 fertile patients, exhibiting normal sperm concentrations and motility, all showed good sperm attachment and penetration rates of 24-89% (Tyler et al 1981). The HOP test has been performed on the male Relationship between sperm motility in semen and the result of the HOP test in 501 men.…”

Section: Relationship To Other Semen Testsmentioning

confidence: 99%

Clinical experience of the heterologous oocyte penetration test 1979‐1985

Pryor¹

1986

Int J of Andrology

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The heterologous oocyte penetration (HOP) test has been under evaluation in our laboratory for the last six years and has shown itself to be a useful technique in the assessment of couples with unexplained infertility. The following paper summarises our experience. et al. (1981) considered the basic techniques for the HOP test and preferred to collect the motile spermatozoa by layering 0.5 ml of semen under 5 ml of BWW (Biggers et al. 1971) supplemented by the addition of 20 mM-HEPES and 3 mg HSA/ml. After 1-1y2 h the motile spermatozoa were concentrated from the supernatant by centrifugation and washed twice more before pre-incubation. A final sperm concentration of 1 to 10 x 106 spermatozoa/ml was obtained and incubated for 18 h overnight at 37°C in a tightly stoppered bottle. An atmosphere of air (pH medium 8.2) was preferable to one of 95% air: 5% COz (pH medium 7.2). Materials and Methods TylerThe recovery of oocytes was more predictable from super ovulated-immature (25-30 day old) golden hamsters (Mesocricetus auratus) than from the culture of immature oocytes. Super-ovulation was induced by an intraperitoneal injection of 40 IU of pregnant mares' serum gonadotropin, followed 72 h later by 25 IU of 76

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Heterologous ovum penetration by human spermatozoa

Cited by 57 publications

References 13 publications

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Relationship Between the Sperm Penetration Assay and Other Tests of Sperm Function

Clinical experience of the heterologous oocyte penetration test 1979‐1985

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