Clinical experience of the heterologous oocyte penetration test 1979‐1985

Abstract

The heterologous oocyte penetration (HOP) test has been under evaluation in our laboratory for the last six years and has shown itself to be a useful technique in the assessment of couples with unexplained infertility. The following paper summarises our experience. et al. (1981) considered the basic techniques for the HOP test and preferred to collect the motile spermatozoa by layering 0.5 ml of semen under 5 ml of BWW (Biggers et al. 1971) supplemented by the addition of 20 mM-HEPES and 3 mg HSA/ml. After 1-1y2 h the motile spermatozoa were concentrated from the supernatant by centrifugation and washed twice more before pre-incubation. A final sperm concentration of 1 to 10 x 106 spermatozoa/ml was obtained and incubated for 18 h overnight at 37°C in a tightly stoppered bottle. An atmosphere of air (pH medium 8.2) was preferable to one of 95% air: 5% COz (pH medium 7.2). Materials and Methods TylerThe recovery of oocytes was more predictable from super ovulated-immature (25-30 day old) golden hamsters (Mesocricetus auratus) than from the culture of immature oocytes. Super-ovulation was induced by an intraperitoneal injection of 40 IU of pregnant mares' serum gonadotropin, followed 72 h later by 25 IU of 76