Heterologous expression of Paranosema (Antonospora) locustae hexokinase in lepidopteran, Sf9, cells is followed by accumulation of the microsporidian protein in insect cell nuclei

Timofeev, Sergey A.; Senderskiy, Igor V.; Tsarev, Alexander; Tokarev, Yuri S.; Dolgikh, Viacheslav V.

doi:10.1016/j.jip.2016.12.002

Cited by 12 publications

(10 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The localization of eukaryotic expressed rHK revealed that the signal peptide could not lead the rHK to be secreted or to enter into nuclei of healthy BmN or Sf9-III. The localization results were different from those of P. locustae HK which is mainly concentrated on hosts’ nuclei ( Senderskiy et al, 2014 ; Timofeev et al, 2017 ). Expression profiles in vivo and in vitro also indicated NbHK continuous high transcriptional level during proliferation stages of N. bombycis infection and the western blot showed NbHK barely exist in mature spores, which implied the NbHK just functioned in intracellular stage.…”

Section: Discussioncontrasting

confidence: 67%

“…This phenomenon was reported in Paranosema (Antonospora) locustae as well. The P. locustae HK was predicted to contain a signal peptide, and the indirect immunofluorescent assay (IFA) showed a nuclear localization in the host ( Senderskiy et al, 2014 ; Timofeev et al, 2017 ). Then, special secreting hexokinase function which work as a regulator to increase ATP generation to parasite surface, was explored in Trachipleistophora hominis ( Ferguson & Lucocq, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A secretory hexokinase plays an active role in the proliferation of Nosema bombycis

Huang

Zheng

Mei

et al. 2018

PeerJ

View full text Add to dashboard Cite

The microsporidian Nosema bombycis is an obligate intracellular parasite of Bombyx mori, that lost its intact tricarboxylic acid cycle and mitochondria during evolution but retained its intact glycolysis pathway. N. bombycis hexokinase (NbHK) is not only a rate-limiting enzyme of glycolysis but also a secretory protein. Indirect immunofluorescence assays and recombinant HK overexpressed in BmN cells showed that NbHK localized in the nucleus and cytoplasm of host cell during the meront stage. When N. bombycis matured, NbHK tended to concentrate at the nuclei of host cells. Furthermore, the transcriptional profile of NbHK implied it functioned during N. bombycis’ proliferation stages. A knock-down of NbHK effectively suppressed the proliferation of N. bombycis indicating that NbHK is an important protein for parasite to control its host.

show abstract

Section: Discussioncontrasting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

A secretory hexokinase plays an active role in the proliferation of Nosema bombycis

Huang

Zheng

Mei

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…Hexokinase ( HK ) is the first enzyme in the glucose metabolism pathway, as well as a multifunctional protein with roles in transcriptional regulation (Niederacher and Entian 1991, Herrero et al 1995) and apoptosis (Gottlob et al 2001, Bryson et al 2002), as well as acting as a cytokine neurotransmitter interleukin (neurotrophic factor) (Gurney et al 1986, Chaput et al 1988, Faik et al 1988). In addition, HK is also involved in pathogen-host interactions; for example, HK was first reported in Nematocida parisii (Cuomo et al 2012) and has also been shown in Paranosema (Antonospora) locustaes by biochemical experiments (Reinke et al 2017), and in P. locustaes (Senderskiy et al 2014, Timofeev et al 2017). Locustae HK is predicted to contain a signal peptide, while showing the presence of HK in the host’s indirect immunofluorescence assay (IFA) (Senderskiy et al 2014, Timofeev et al 2017).…”

mentioning

confidence: 99%

Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper

Pan

et al. 2019

Journal of Insect Science

View full text Add to dashboard Cite

Glucose-6-phosphatase (G6Pase) and hexokinase (HK) are two key enzymes in the glycolysis and gluconeogenesis pathways, which catalyze the synthesis and degradation of glucose in insects, respectively. G6Pase and HK play an important role in insect growth by regulating the metabolism of glucose, leading to the efficient metabolism of other macromolecules. However, it is unclear whether these genes could be investigated for pest control through their actions on chitin metabolism. We studied the potential functions of G6Pase and HK genes in the regulation of chitin metabolism pathways by RNAi technology. Interference with G6Pase expression did not affect trehalose and chitin metabolism pathways in brown planthopper, Nilaparvata lugens (Stål). However, knockdown of the HK gene resulted in a significant decrease of expression of genes associated with the trehalose metabolic pathway but had no significant effect on trehalase activity, trehalose content, or glucogen content. Additionally, HK knockdown resulting in downregulation of the genes involved in chitin metabolism in the brown planthopper. These insects also showed wing deformities and difficulty in molting to varying degrees. We suggest that the silencing of HK expression directly inhibited the decomposition of glucose, leading to impaired chitin synthesis.

show abstract

“…; Timofeev et al. ). However, little is known about the molecular pathogenicity of A. locustae , a potentially intriguing model system for understanding the extremes of reductive parasite evolution and host cell manipulation (Williams ).…”

mentioning

confidence: 98%

“…The sporewall and polar-tube proteins are transported from the endoplasmic reticulum to the target membranes through these tubular networks (Beznoussenko et al 2007). In aspects of host-parasite interactions, the localization of hexokinase secreted by A. locustae into infected host cells suggests that some of Microsporidia possess a broad set of enzymes and regulatory proteins that have the potential to alter metabolic processes and molecular programs of the host (Senderskiy et al 2014;Timofeev et al 2016). However, little is known about the molecular pathogenicity of A. locustae, a potentially intriguing model system for understanding the extremes of reductive parasite evolution and host cell manipulation (Williams 2009). In this paper, we have identified a putative spore wall protein of A. locustae via MALDI-TOF mass spectrometry.…”

mentioning

confidence: 99%

A Novel Spore Wall Protein from Antonospora locustae (Microsporidia: Nosematidae) Contributes to Sporulation

Chen

Zhao

You

et al. 2017

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Microsporidia are obligate intracellular parasites, existing in a wide variety of animal hosts. Here, we reported Aloc SWP2, a novel protein identified from the spore wall of Antonospora locustae (formerly, Nosema locustae, and synonym, Paranosema locustae), containing four cysteines that are conserved among the homologues of several Microspodian pathogens in insects and mammals. Aloc SWP2 was detected in the wall of mature spores via indirect immunofluorescence assay. In addition, immunocytochemistry localization experiments showed that the protein was observed in the wall of sporoblasts, sporonts, and meronts during sporulation within the host body, also in the wall of mature spores. AlocSWP2 was not detected in the fat body of infected locust until the 9th day after inoculating spores via RT‐PCR experiments. Furthermore, the survival percentage of infected locusts injected with dsRNA of AlocSWP2 on the 15th, 16th, and 17th days after inoculation with microsporidian were significantly higher than those of infected locusts without dsRNA treatment. Conversely, the amount of spores in locusts infected with A. locustae after treated with RNAi AlocSWP2 was significantly lower than those of infected locusts without RNAi of this gene. This novel spore wall protein from A. locustae may be involved in sporulation, thus contributing to host mortality.

show abstract

Heterologous expression of Paranosema (Antonospora) locustae hexokinase in lepidopteran, Sf9, cells is followed by accumulation of the microsporidian protein in insect cell nuclei

Cited by 12 publications

References 15 publications

A secretory hexokinase plays an active role in the proliferation of Nosema bombycis

A secretory hexokinase plays an active role in the proliferation of Nosema bombycis

Glucose Utilization in the Regulation of Chitin Synthesis in Brown Planthopper

A Novel Spore Wall Protein from Antonospora locustae (Microsporidia: Nosematidae) Contributes to Sporulation

Contact Info

Product

Resources

About