Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis

Jing, Shaojuan; Zhou, Xiang; Song, Yu; Yu, Diqiu

doi:10.1007/s10725-009-9366-z

Cited by 92 publications

(60 citation statements)

References 51 publications

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“…Although the similarity between AtWRKY22 protein and At-WRKY53, AtWRKY6, OsWRKY23 proteins is not high, the relative high expression level of AtWRKY22 was found to be the same as AtWRKY53 and OsWRKY23 under the condition of dark-induced senescence (Jing et al, 2009;Miao et al, 2004) (Figs. 2D and 7D).…”

Section: Resultsmentioning

confidence: 99%

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WRKY22 Transcription Factor Mediates Dark-Induced Leaf Senescence in Arabidopsis

Zhou

Jiang

2011

Molecules and Cells

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261

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Arabidopsis WRKY proteins are plant-specific transcription factors, encoded by a large gene family, which contain the highly conserved amino acid sequence WRKYGQK and the zinc-finger-like motifs, Cys 2 His 2 or Cys 2 HisCys. They can recognize and bind the TTGAC(C/T) W-box ciselements found in the promoters of target genes, and are involved in the regulation of gene expression during pathogen defense, wounding, trichome development, and senescence. Here we investigated the physiological function of the Arabidopsis WRKY22 transcription factor during dark-induced senescence. WRKY22 transcription was suppressed by light and promoted by darkness. In addition, AtWRKY22 expression was markedly induced by H 2 O 2 . These results indicated that AtWRKY22 was involved in signal pathways in response to abiotic stress. Darktreated AtWRKY22 over-expression and knockout lines showed accelerated and delayed senescence phenotypes, respectively, and senescence-associated genes exhibited increased and decreased expression levels. Mutual regulation existed between AtWRKY22 and AtWRKY6, AtWR-KY53, and AtWRKY70, respectively. Moreover, AtWRKY22 could influence their relative expression levels by feedback regulation or by other, as yet unknown mechanisms in response to dark. These results prove that AtWRKY22 participates in the dark-induced senescence signal transduction pathway.

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Section: Resultsmentioning

confidence: 99%

“…Our previous work showed that the expression of Oryza sativa WRKY23 was induced by the dark and that heterologous expression of OsWRKY23 enhanced dark-induced leaf senescence in Arabidopsis (Jing et al, 2009). In addition, certain Arabidopsis WRKY genes have important roles in dark-induced senescence signal transduction.…”

Section: Discussionmentioning

confidence: 99%

WRKY22 Transcription Factor Mediates Dark-Induced Leaf Senescence in Arabidopsis

Zhou

Jiang

2011

Molecules and Cells

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261

181

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“…Numerous studies have reported that expression of WRKY genes in plants is induced by abiotic stresses (Rizhsky et al 2002;Ulker and Somssich 2004;Marchive et al 2007;Jing et al 2009). In the present study, VpWRKY1 and VpWRKY2 transgenic Arabidopsis plants are more capable of adapting to the salt stress during germination (Fig.…”

Section: Discussionmentioning

confidence: 99%

Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata

Xiao

et al. 2010

Planta

110

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In this study, two WRKY genes were isolated from Erysiphe necator-resistant Chinese wild Vitis pseudoreticulata W. T. Wang 'Baihe-35-1', and designated as VpWRKY1 (GenBank accession no. GQ884198) and VpWRKY2 (GenBank accession no. GU565706). Nuclear localization of the two proteins was demonstrated in onion epidermal cells, while trans-activation function was confirmed in the leaves of 'Baihe-35-1'. Expression of VpWRKY1 and VpWRKY2 was induced rapidly by salicylic acid treatment in 'Baihe-35-1'. Expression of VpWRKY1 and VpWRKY2 was also induced rapidly by E. necator infection in 11 grapevine genotypes; the maximum induction of VpWRKY1 was greater in E. necator-resistant grapevine genotypes than in susceptible ones post E. necator inoculation. Furthermore, ectopic expression of VpWRKY1 or VpWRKY2 in Arabidopsis enhanced resistance to powdery mildew Erysiphe cichoracearum, and enhanced salt tolerance of transgenic plants. VpWRKY2 also enhanced cold tolerance of transgenic plants. In addition, the two proteins were shown to regulate the expression of some defense marker genes in Arabidopsis and grapevine. The data suggest that VpWRKY1 and VpWRKY2 may underlie the resistance in transgenic grapevine to E. necator and tolerance to salt and cold stresses.

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“…Recent studies have indicated that WRKY transcription factors also play important roles in physiological and developmental programs, including senescence, seed germination and trichome development (Hinderhofer and Zentgraf 2001;Johnson et al 2002;Singh et al 2002). OsWRK23 enhances dark-induced leaf senescence in Arabidopsis (Jing et al 2009), while AtWRKY22 participates in the dark-induced senescence signal-transduction pathway (Zhou et al 2011). AtWRKY2 mediates seed germination and post-germination arrest of development by ABA (Jiang and Yu 2009), and AtWRKY57 functions as a node of convergence for jasmonic acid-and auxin-mediated signaling in jasmonic acid-induced leaf senescence (Jiang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement

Sun

2015

Plant Cell Rep

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105

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AtWRKY53 is an early factor in drought response and activated expression of AtWRKY53 regulates stomatal movement via reduction of H 2 O 2 content and promotion of starch metabolism in guard cells. Drought is one of the most serious environmental factors limiting the productivity of agricultural crops worldwide. However, the mechanisms underlying drought tolerance in plants remain unclear. AtWRKY53 belongs to the group III of WRKY transcription factors. In this study, we observed both the mRNA and protein products of this gene are rapidly induced under drought conditions. Phenotypic analysis showed AtWRKY53 overexpression lines were hypersensitive to drought stress compared with Col-0 plants. The results of stomatal movement assays and abscisic acid (ABA) content detection indicated that the impaired stomatal closure of 53OV lines was independent of ABA. Further analysis found that WRKY53 regulated stomatal movement via reducing the H2O2 content and promoting the starch metabolism in guard cells. The results of quantitative real-time reverse transcriptase PCR showed that the expression levels of CAT2, CAT3 and QQS were up-regulated in 53OV lines. Chromatin immunoprecipitation assays demonstrated that AtWRKY53 can directly bind to the QQS promoter sequences, thus led to increased starch metabolism. In summary, our results indicated that the activated expression of AtWRKY53 inhibited stomatal closure by reducing H2O2 content and facilitated stomatal opening by promoting starch degradation.

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Heterologous expression of OsWRKY23 gene enhances pathogen defense and dark-induced leaf senescence in Arabidopsis

Cited by 92 publications

References 51 publications

WRKY22 Transcription Factor Mediates Dark-Induced Leaf Senescence in Arabidopsis

WRKY22 Transcription Factor Mediates Dark-Induced Leaf Senescence in Arabidopsis

Expression and functional analysis of two genes encoding transcription factors, VpWRKY1 and VpWRKY2, isolated from Chinese wild Vitis pseudoreticulata

Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement

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