Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement

Sun, Yawang; Yu, Diqiu

doi:10.1007/s00299-015-1787-8

Cited by 105 publications

(79 citation statements)

References 56 publications

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“…The nucleus localization after translation and the up-regulated expression pattern upon drought and exogenous ABA indicates that TaWRKY1 is possibly involved in mediating drought responses through an ABA-dependent pathway. Several lines of evidence have shown that a subset of the WRKY family genes are functional in mediating plant tolerance to drought stress, through their roles in modulating the physiological processes associated with defensiveness of drought or related osmotic stresses (Ishiguro and Nakamura 1994;Niu et al 2012;Wang et al 2013;Sun and Yu 2015;Jia et al 2015). For instance, TaWRKY2, TaWRKY19, and TaWRKY10, the WRKY family genes in wheat, function effectively in responses to a set of abiotic stresses, such as drought, high salinity, low temperature, wounding, and the external ABA application via improvement of osmolyte accumulation and cellular ROS homeostasis (Niu et al 2012;Wang et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…To date, it has been noted that the drought response of plants is associated with the function exerted by a suite of WRKY proteins. For example, Arabidosis AtWRKY53 acts as a significantly negative regulator in drought response through modulation of stomata movement, cellular H 2 O 2 accumulation, and starch biosynthetic metabolism (Sun and Yu 2015). TaWRKY2 and TaWRKY19, two WRKY family genes of wheat, mediate drought tolerance of plants via activation of STZ and DREB2A, two conserved components of the osmotic stress-defensive pathways (Niu et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Wheat WRKY Type Transcription Factor Gene TaWRKY1 is Essential in Mediating Drought Tolerance Associated with an ABA-Dependent Pathway

et al. 2016

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WRKY-type transcription factors (TFs) are important regulators in plant growth, development and responses to diverse biotic and abiotic stresses. In this study, we characterized the function of TaWRKY1, a wheat (Triticum aestivum) WRKY family gene, in mediating plant tolerance to drought. TaWRKY1 bears a conserved WRKY motif and a C2H2 domain and targets onto the nucleus under both normal and simulated drought conditions. On exposure to simulated drought and ABA, the TaWRKY1 expression levels were significantly up-regulated. Ectopic overexpression of TaWRKY1 in tobacco conferred improved tolerance to drought; the transgenic plants exhibited more biomass, slower leaf water loss rate (WLR), more osmolyte accumulation, and higher antioxidant enzyme activities than wild type (WT) plants after drought treatments. In addition, the stomata closure rate was promoted in the transgenic plants upon exposure to drought and -exogenous ABA with respect to that in WT plants, suggesting that TaWRKY1 mediates the stomata movement and impacts the leaf water retention capacity. The transcripts of NtPYL8, an ABA receptor gene in tobacco, were shown to be induced in the TaWRKY1-overexpressing plants under drought and ABA stresses. Moreover, overexpression and knockdown of NtPYL8 modified the stomata movement characterization, leaf WLR, and phenotype of the seedlings upon exposure to the drought stress. These findings suggest that NtPYL8 acts as one of the critical players in transducing ABA signaling initiated by osmotic stress, contributing to the TaWRKY1-mediated drought tolerance via regulating stomata movement and plant water retention capacity. Our findings together reveal that TaWRKY1 plays an important role in mediating the plant tolerance to drought through modification of a set of osmotic stress-defensive processes connecting an ABA-dependent pathway. NtPYL8 and its homolog in wheat are crucial members in transducing the drought-initiated ABA signaling and are functional in modulating stomata movement.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Wheat WRKY Type Transcription Factor Gene TaWRKY1 is Essential in Mediating Drought Tolerance Associated with an ABA-Dependent Pathway

et al. 2016

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“…For example, AtWRKY53 and AtWRKY70 play a positive role in SA-mediated resistance to P . syringae infection [43,44], and OsWRKY45 acts in the SA signaling pathway independently of NPR1 . Our results showed that the expression levels of NPR1 and WRKY45 were reduced in oscol9-ko plants, indicating that OsCOL9 possibly acted upstream of NPR1 (Fig 6A).…”

Section: Discussionmentioning

confidence: 99%

CONSTANS-Like 9 (OsCOL9) Interacts with Receptor for Activated C-Kinase 1(OsRACK1) to Regulate Blast Resistance through Salicylic Acid and Ethylene Signaling Pathways

et al. 2016

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In a previous transcriptome analysis of early response genes in rice during Magnaporthe oryzae infection, we identified a CONSTANS-like (COL) gene OsCOL9. In the present study, we investigated the functional roles of OsCOL9 in blast resistance. OsCOL9 belonged to group II of the COL protein family, and it contained a BB-box and a C-terminal CCT (CONSTANS, COL and TOC1) domain. OsCOL9 was found in the nucleus of rice cells, and it exerted transcriptional activation activities through its middle region (MR). Magnaporthe oryzae infection induced OsCOL9 expression, and transgenic OsCOL9 knock-out rice plants showed increased pathogen susceptibility. OsCOL9 was a critical regulator of pathogen-related genes, especially PR1b, which were also activated by exogenous salicylic acid (SA) and 1-aminocyclopropane-1-carboxylicacid (ACC), the precursor of ethylene (ET). Further analysis indicated that OsCOL9 over-expression increased the expressions of phytohormone biosynthetic genes, NPR1, WRKY45, OsACO1 and OsACS1, which were related to SA and ET biosynthesis. Interestingly, we found that OsCOL9 physically interacted with the scaffold protein OsRACK1 through its CCT domain, and the OsRACK1 expression was induced in response to exogenous SA and ACC as well as M. oryzae infection. Taken together, these results indicated that the COL protein OsCOL9 interacted with OsRACK1, and it enhanced the rice blast resistance through SA and ET signaling pathways.

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“…The stomatal closure assay was performed using leaves harvested from 30-day-old control or transgenic tobacco seedlings as described previously [24]. The leaves were immersed in a solution containing 10 mM KCl, 50μM CaCl 2 , and 10 mM MES-KOH (pH 6.15) under strong light for 6 h. Subsequently, 50 μM ABA or 200 mM mannitol was added to the solution.…”

Section: Methodsmentioning

confidence: 99%

A CBL-Interacting Protein Kinase TaCIPK2 Confers Drought Tolerance in Transgenic Tobacco Plants through Regulating the Stomatal Movement

Wang

Sun

et al. 2016

PLoS ONE

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In plants, the CBL-CIPK signaling pathways play key roles in the response to abiotic stresses. However, functional studies of CIPKs in the important staple crop wheat are very rare. In this study, we identified a CIPK gene from wheat, designated TaCIPK2. Expression analysis results showed that TaCIPK2 could be up-regulated in wheat leaves by polyethylene glycol, abscisic acid and H2O2 treatments. Subcellular localization analyses revealed that TaCIPK2 was present in whole wheat epidermal cells. A yeast two-hybrid assay indicated that TaCIPK2 interacted with TaCBL1, 2, 3 and 4 in vitro. Transgenic tobacco plants over-expressing TaCIPK2 exhibited increased drought tolerance, indicated by a larger proportion of green cotyledons and higher survival rates under the osmotic and drought stress conditions compared with control plants. Additionally, physiological index analyses revealed that the transgenic tobacco plants had lower water loss rates and ion leakage, accumulated less malondialdehyde and H2O2, and had higher catalase and superoxide dismutase activities than the control plants. The transgenic plants also exhibited faster stomatal closure following exposure to osmotic stress conditions. The seed germination rates and stomatal aperture of TaCIPK2-overexpressing tobacco plants decreased after exogenous abscisic acid treatment was applied, implying that the transgenic tobacco plants were more sensitive to exogenous abscisic acid than the control plants. Our results indicate that TaCIPK2 plays a positive regulatory role in drought stress responses in transgenic tobacco plants.

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Activated expression of AtWRKY53 negatively regulates drought tolerance by mediating stomatal movement

Cited by 105 publications

References 56 publications

Wheat WRKY Type Transcription Factor Gene TaWRKY1 is Essential in Mediating Drought Tolerance Associated with an ABA-Dependent Pathway

Wheat WRKY Type Transcription Factor Gene TaWRKY1 is Essential in Mediating Drought Tolerance Associated with an ABA-Dependent Pathway

CONSTANS-Like 9 (OsCOL9) Interacts with Receptor for Activated C-Kinase 1(OsRACK1) to Regulate Blast Resistance through Salicylic Acid and Ethylene Signaling Pathways

A CBL-Interacting Protein Kinase TaCIPK2 Confers Drought Tolerance in Transgenic Tobacco Plants through Regulating the Stomatal Movement

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