Heterologous expression of maize chloroplast protein synthesis elongation factor (EF-Tu) enhances Escherichia coli viability under heat stress

Proteomics combines morphological, physiological and biochemical attributes to unravel the survival strategy of Anabaena sp. PCC7120 under arsenic stress

“…[107,108]. Our results suggest that overexpressed EF-Tu might offer tolerance against As stress as also known for maize [109] and E. coli under heat stress [110], Pseudomonas fluorescens under cobalt stress [108] and A. doliolum under salt stress [8].…”

Expression of chloroplast protein synthesis elongation factor, EF-Tu, in two lines of maize with contrasting tolerance to heat stress during early stages of plant development

“…Previous studies have shown: (a) positive correlation between the heat-induced accumulation of EFTu and plant ability to tolerate heat stress in several genotypes of maize (Bhadula et al, 2001;Ristic et al, 1996), (b) association between the heat-induced synthesis of EF-Tu and the maize heat tolerance phenotype (Ristic et al, 1998); [in the previous studies (Ristic et al, 1996(Ristic et al, , 1998) maize EF-Tu was referred to as 45-46 kD HSP because the identity of this protein was not known until the report of Bhadula et al (2001)], (c) increased tolerance to heat stress in Escherichia coli expressing maize EF-Tu (Moriarty et al, 2002), (d) decreased tolerance to heat stress in a maize mutant with reduced capacity to accumulate EF-Tu , (e) decreased thermal aggregation of chloroplast proteins in maize with higher levels of EF-Tu Ristic et al, 2004), and (f) chaperone activity of recombinant maize EF-Tu; maize EF-Tu was shown to protect heat-labile proteins from thermal aggregation and inactivation by acting as a molecular chaperone (Rao et al, 2004). Rao et al (2004) have proposed a hypothesis that maize EF-Tu plays a role in the development of heat tolerance.…”

“…In contrast, no heat-induced synthesis of EF-Tu is found in a heat sensitive maize line, ZPL 389 . Maize EF-Tu has been implicated in the development of heat tolerance Moriarty et al, 2002;Rao et al, 2004;, and it has been suggested that this protein acts as a molecular chaperone and protects chloroplast proteins from thermal aggregation and inactivation (Rao et al, 2004).…”

Heterologous expression of a plastid EF-Tu reduces protein thermal aggregation and enhances CO2 fixation in wheat (Triticum aestivum) following heat stress

“…A recent study has revealed that in wheat, this protein is upregulated under heat stress conditions and those wheat genotypes that accumulate more EF-Tu display better tolerance to heat stress (Ristic et al 2008). Previous studies on EF-Tu in maize have revealed (a) positive correlation between the heat-induced accumulation of EF-Tu and plant ability to tolerate heat stress (Bhadula et al 2001;Momcilovic and Ristic 2007), (b) increased thermotolerance in Escherichia coli expressing maize EF-Tu (Moriarty et al 2002), (c) decreased thermotolerance in a maize mutant with reduced capacity to express EF-Tu , and (d) reduced thermal aggregation of chloroplast proteins in maize with higher levels of EF-Tu Momcilovic and Ristic 2004). It has been hypothesized that EF-Tu confers heat tolerance by acting as a molecular chaperone (Rao et al 2004).…”