Heterologous expression of IbMYB1a by different promoters exhibits different patterns of anthocyanin accumulation in tobacco

An, Chul Han; Lee, Ki-Won; Lee, Sanghoon; Jeong, Yu Jeong; Woo, Su Gyoung; Chun, Hyo Kon; Park, Youn‐Il; Kwak, Sang‐Soo; Kim, Cha Young

doi:10.1016/j.plaphy.2015.02.002

Cited by 32 publications

(17 citation statements)

References 50 publications

(68 reference statements)

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“…The unigene c5221-g1 was annotated as IbMYB1 and was expressed at a 4.6-fold higher level in Js57-CK than in XZH-CK, indicating its important potential function in the defense response. In sweet potato, MYB1 (IbMYB1) stimulates the accumulation of anthocyanin pigmentation in the tuberous roots of sweet potato and the leaves of tobacco and arabidopsis [ 54 – 56 ]. IbMYB1 also improves tolerance and enhances secondary metabolism in transgenic potatoes [ 57 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome profiling and digital gene expression analysis of sweet potato for the identification of putative genes involved in the defense response against Fusarium oxysporum f. sp. batatas

et al. 2017

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Sweet potato production is constrained by Fusarium wilt, which is caused by Fusarium oxysporum f. sp. batatas (Fob). The identification of genes related to disease resistance and the underlying mechanisms will contribute to improving disease resistance via sweet potato breeding programs. In the present study, we performed de novo transcriptome assembly and digital gene expression (DGE) profiling of sweet potato challenged with Fob using Illumina HiSeq technology. In total, 89,944,188 clean reads were generated from 12 samples and assembled into 101,988 unigenes with an average length of 666 bp; of these unigenes, 62,605 (61.38%) were functionally annotated in the NCBI non-redundant protein database by BLASTX with a cutoff E-value of 10−5. Clusters of Orthologous Groups (COG), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations were examined to explore the unigenes’ functions. We constructed four DGE libraries for the sweet potato cultivars JinShan57 (JS57, highly resistant) and XinZhongHua (XZH, highly susceptible), which were challenged with pathogenic Fob. Genes that were differentially expressed in the four libraries were identified by comparing the transcriptomes. Various genes that were differentially expressed during defense, including chitin elicitor receptor kinase 1 (CERK), mitogen-activated protein kinase (MAPK), WRKY, NAC, MYB, and ethylene-responsive transcription factor (ERF), as well as resistance genes, pathogenesis-related genes, and genes involved in salicylic acid (SA) and jasmonic acid (JA) signaling pathways, were identified. These data represent a sequence resource for genetic and genomic studies of sweet potato that will enhance the understanding of the mechanism of disease resistance.

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Section: Resultsmentioning

confidence: 99%

Transcriptome profiling and digital gene expression analysis of sweet potato for the identification of putative genes involved in the defense response against Fusarium oxysporum f. sp. batatas

et al. 2017

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“…Ectopic expression of anthocyanin biosynthetic regulators of transgenic plants has been reported to lead to visible pigment accumulation in the leaves, stems, flowers and roots [ 15 , 16 , 29 ]. In some cases, high accumulation of anthocyanins in transgenic tobacco has had a negative effect on plant growth and seed development [ 15 , 16 ]. In this study, the expression of two anthocyanin regulators under the control of a flower-specific promoter, PANS, led to a higher anthocyanin accumulation in floral organs specifically, while displaying a similar growth phenotype to the NT plants ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…Expression of the sweet potato IbMYB1a (R2R3 MYB) gene, as well as co-expression of the maize B-peru gene with the Arabidopsis mPAP1 gene, in transgenic tobacco plants results in notable color changes throughout the plant body including callus, seedling, roots, stems, leaves and flowers. These tobacco plants accumulate anthocyanins, but also display a growth-retardation phenotype [ 15 , 16 , 17 ], suggesting that high accumulation of anthocyanins may have a deleterious effect on plant growth.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter

Kim

Park

Lee

et al. 2018

IJMS

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Flower color is a main target for flower breeding. A transgenic approach for flower color modification requires a transgene and a flower-specific promoter. Here, we expressed the B-peru gene encoding a basic helix loop helix (bHLH) transcription factor (TF) together with the mPAP1 gene encoding an R2R3 MYB TF to enhance flower color in tobacco (Nicotiana tabacum L.), using the tobacco anthocyanidin synthase (ANS) promoter (PANS) to drive flower-specific expression. The transgenic tobacco plants grew normally and produced either dark pink (PANSBP_DP) or dark red (PANSBP_DR) flowers. Quantitative real time polymerase chain reaction (qPCR) revealed that the expression of five structural genes in the flavonoid biosynthetic pathway increased significantly in both PANSBP_DP and PANSBP_DR lines, compared with the non-transformed (NT) control. Interestingly, the expression of two regulatory genes constituting the active MYB-bHLH-WD40 repeat (WDR) (MBW) complex decreased significantly in the PANSBP_DR plants but not in the PANSBP_DP plants. Total flavonol and anthocyanin abundance correlated with flower color, with an increase of 1.6–43.2 fold in the PANSBP_DP plants and 2.0–124.2 fold in the PANSBP_DR plants. Our results indicate that combinatorial expression of B-peru and mPAP1 genes under control of the ANS promoter can be a useful strategy for intensifying flower color without growth retardation.

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“…Anthocyanins, the largest subclass of water-soluble pigments synthesized via the flavonoid pathway, confer appealing color present in plants [1,2]. Anthocyanins play important roles in a large range of plant functions, such as providing protection against UV radiation, low temperature, drought stress and pathogen attack [3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.)

et al. 2016

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Eggplant is rich in anthocyanins, which are the major secondary metabolites and beneficial to human health. We discovered that the anthocyanin biosynthesis of eggplant cultivar 'Lanshan Hexian' was regulated by light. In this study, we isolated two blue light receptor genes, SmCRY1 and SmCRY2, and negative/positive anthocyanin regulatory factors SmCOP1 and SmHY5 from eggplant. In terms of transcript levels, SmCRY1, SmCRY2 and SmHY5 were up-regulated by light, while SmCOP1 was down-regulated. Subsequently, the four genes were functionally complemented in phenotype of corresponding mutants, indicating that they act as counterparts of Arabidopsis genes. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that SmCRY1 and SmCRY2 interact with SmCOP1 in a blue-light-dependent manner. It also obtained the result that SmCOP1 interacts with SmHY5 and SmMYB1. Furthermore, using yeast one-hybrid assay, we found that SmHY5 and SmMYB1 both bind the promoters of anthocyanin biosynthesis structural genes (SmCHS and SmDFR). Taken together, blue-light-triggered CRY1/CRY2-COP1 interaction creates the condition that HY5 and MYB1 combine with the downstream anthocyanin synthesis genes (CHS and DFR) in eggplant. Our finding provides a new working model by which light controls anthocyanin accumulation in eggplant.

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Heterologous expression of IbMYB1a by different promoters exhibits different patterns of anthocyanin accumulation in tobacco

Cited by 32 publications

References 50 publications

Transcriptome profiling and digital gene expression analysis of sweet potato for the identification of putative genes involved in the defense response against Fusarium oxysporum f. sp. batatas

Transcriptome profiling and digital gene expression analysis of sweet potato for the identification of putative genes involved in the defense response against Fusarium oxysporum f. sp. batatas

Enhancing Flower Color through Simultaneous Expression of the B-peru and mPAP1 Transcription Factors under Control of a Flower-Specific Promoter

Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.)

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