Heterologous Expression of BoPAP1 in Tomato Induces Stamen Specific Anthocyanin Accumulation and Enhances Tolerance to a Long-Term Low Temperature Stress

Ren, Lijun; Hu, Zongli; Li, Yali; Zhang, Bin; Zhang, Yanjie; Tu, Yun; Chen, Guoping

doi:10.1007/s00344-014-9423-3

Cited by 23 publications

(11 citation statements)

References 53 publications

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“…Overexpression of the SlAN2 in tomato led to the same phenotype in vegetative tissues as SlANT1 , but unexpectedly, no anthocyanin accumulation was noted in tomato fruits 62 . Heterologous expression of BoPAP1 in tomato induced stamen-specific anthocyanin accumulation, and observed the consistency of SlTT8 expression and anthocyanin accumulation in purple stamens 63 . Together, these results lead to the conclusion that low expression of SlANT1 , SlAN2 , and SlTT8 in tomato fruits might be responsible for the low accumulation of anthocyanins in WT and SlAN11 overexpression fruits, and modification of only one MBW member, like SlAN11 , SlTT8 , SlANT1 , and SlAN2 , causes little effects in the fruit anthocyanin contents.…”

Section: Discussionmentioning

confidence: 75%

Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins

Liu

Chen

et al. 2018

Hortic Res

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The flavonoid compounds are important secondary metabolites with versatile human nutritive benefits and fulfill a multitude of functions during plant growth and development. The abundance of different flavonoid compounds are finely tuned with species-specific pattern by a ternary MBW complex, which consists of a MYB, a bHLH, and a WD40 protein, but the essential role of SlAN11, which is a WD40 protein, is not fully understood in tomato until now. In this study, a tomato WD40 protein named as SlAN11 was characterized as an effective transcription regulator to promote plant anthocyanin and seed proanthocyanidin (PA) contents, with late flavonoid biosynthetic genes activated in 35S::SlAN11 transgenic lines, while the dihydroflavonol flow to the accumulation of flavonols or their glycosylated derivatives was reduced by repressing the expression of SlFLS in this SlAN11-overexpressed lines. The above changes were reversed in 35S::SlAN11-RNAi transgenic lines except remained levels of flavonol compounds and SlFLS expression. Interestingly, our data revealed that SlAN11 gene could affect seed dormancy by regulating the expressions of abscisic acid (ABA) signaling-related genes SlABI3 and SlABI5, and the sensitivity to ABA treatment in seed germination is conversely changed by SlAN11-overexpressed or -downregulated lines. Yeast two-hybrid assays demonstrated that SlAN11 interacted with bHLH but not with MYB proteins in the ternary MBW complex, whereas bHLH interacted with MYB in tomato. Our results indicated that low level of anthocyanins in tomato fruits, with low expression of bHLH (SlTT8) and MYB (SlANT1 and SlAN2) genes, remain unchanged upon modification of SlAN11 gene alone in the transgenic lines. These results suggest that the tomato WD40 protein SlAN11, coordinating with bHLH and MYB proteins, plays a crucial role in the fine adjustment of the flavonoid biosynthesis and seed dormancy in tomato.

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Section: Discussionmentioning

confidence: 75%

Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins

Liu

Chen

et al. 2018

Hortic Res

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“…The bHLH proteins function as anthocyanin biosynthesis regulators and have been reported in model plants and vegetable species, including Arabidopsis [ 61 ], tobacco [ 62 , 63 ], red cabbage [ 64 ], tomato [ 65 ], and Chinese cabbage [ 66 ]. These proteins usually serve as co-factors interacting with R2R3-MYBs to induce anthocyanin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis)

Xie

Yuan

et al. 2018

IJMS

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Cytoplasmic male sterility (CMS) is universally utilized in cruciferous vegetables. However, the Chinese cabbage hau CMS lines, obtained by interspecific hybridization and multiple backcrosses of the Brassica juncea (B. juncea) CMS line and Chinese cabbage, show obvious leaf etiolation, and the molecular mechanism of etiolation remains elusive. Here, the ultrastructural and phenotypic features of leaves from the Chinese cabbage CMS line 1409A and maintainer line 1409B are analyzed. The results show that chloroplasts of 1409A exhibit abnormal morphology and distribution. Next, RNA-sequencing (RNA-Seq) is used to identify 485 differentially expressed genes (DEGs) between 1409A and 1409B, and 189 up-regulated genes and 296 down-regulated genes are found. Genes that affect chloroplasts development, such as GLK1 and GLK2, and chlorophyll biosynthesis, such as PORB, are included in the down-regulated DEGs. Quantitative real-time PCR (qRT-PCR) analysis validate that the expression levels of these genes are significantly lower in 1409A than in 1409B. Taken together, these results demonstrate that leaf etiolation is markedly affected by chloroplast development and pigment biosynthesis. This study provides an effective foundation for research on the molecular mechanisms of leaf etiolation of the hau CMS line in Chinese cabbage (Brassica rapa L. ssp. pekinensis).

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“…Anthocyanins, the largest subclass of water-soluble pigments synthesized via the flavonoid pathway, confer appealing color present in plants [1,2]. Anthocyanins play important roles in a large range of plant functions, such as providing protection against UV radiation, low temperature, drought stress and pathogen attack [3][4][5][6]. These pigments also help human to prevent several chronic diseases, such as cancer, cardiovascular disease, obesity and diabetes [7].…”

Section: Introductionmentioning

confidence: 99%

Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.)

et al. 2016

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Eggplant is rich in anthocyanins, which are the major secondary metabolites and beneficial to human health. We discovered that the anthocyanin biosynthesis of eggplant cultivar 'Lanshan Hexian' was regulated by light. In this study, we isolated two blue light receptor genes, SmCRY1 and SmCRY2, and negative/positive anthocyanin regulatory factors SmCOP1 and SmHY5 from eggplant. In terms of transcript levels, SmCRY1, SmCRY2 and SmHY5 were up-regulated by light, while SmCOP1 was down-regulated. Subsequently, the four genes were functionally complemented in phenotype of corresponding mutants, indicating that they act as counterparts of Arabidopsis genes. Yeast two-hybrid and bimolecular fluorescence complementation assays showed that SmCRY1 and SmCRY2 interact with SmCOP1 in a blue-light-dependent manner. It also obtained the result that SmCOP1 interacts with SmHY5 and SmMYB1. Furthermore, using yeast one-hybrid assay, we found that SmHY5 and SmMYB1 both bind the promoters of anthocyanin biosynthesis structural genes (SmCHS and SmDFR). Taken together, blue-light-triggered CRY1/CRY2-COP1 interaction creates the condition that HY5 and MYB1 combine with the downstream anthocyanin synthesis genes (CHS and DFR) in eggplant. Our finding provides a new working model by which light controls anthocyanin accumulation in eggplant.

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Heterologous Expression of BoPAP1 in Tomato Induces Stamen Specific Anthocyanin Accumulation and Enhances Tolerance to a Long-Term Low Temperature Stress

Cited by 23 publications

References 53 publications

Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins

Tomato SlAN11 regulates flavonoid biosynthesis and seed dormancy by interaction with bHLH proteins but not with MYB proteins

Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis)

Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.)

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