Heterologous Encapsidation of Potato Virus Y Strain O (PVYO) With The Transgenic Coat Protein Of PVY Strain N (PVYN) In Solanum Tuberosum Cv. Bintje

Farinelli, Laurent; Malnoë, Pia; Collet, Gérald F.

doi:10.1038/nbt0992-1020

Cited by 67 publications

(28 citation statements)

References 29 publications

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“…Farinelli et al (1992) used strain-specific antibodies and reported that accumulation of transgenic CP from PVY N increased upon inoculation of the plants with PVY O . In our study, the distinct molecular size of the NIa-TMV CP fusion protein allowed us to distinguish between transgenic products and those resulting from virus infection.…”

Section: Increased Accumulation Of Transgenic Proteins Upon Infectionmentioning

confidence: 99%

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Marcos

Beachy²

1997

Journal of General Virology

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An expression cassette based on the highly specific tobacco etch potyvirus (TEV) nuclear inclusion (NIa) proteinase has been developed to produce multiple proteins through the translation of a single selfprocessing polypeptide. Gene constructs encoding TEV NIa, the tobacco mosaic tobamovirus (TMV) coat protein (CP) and the soybean mosaic potyvirus (SMV) CP were used to develop transgenic tobacco plants. Proper processing of the multifunctional polypeptide was demonstrated, leading to accumulation of separate proteins in planta. Moreover, the viral genes expressed in this way were biologically

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Section: Increased Accumulation Of Transgenic Proteins Upon Infectionmentioning

confidence: 99%

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Marcos

Beachy²

1997

Journal of General Virology

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“…In the case of PVY, there have been two reports (Farinelli et al 1992; van der Vlugt and Goldbach 1993) where a higher level of resistance was obtained against isolates of a homologous strain group (PVY N ) than against isolates of a heterologous group (PVY O ) and one report where the heterologous resistance appeared to be complete (Farinelli and Malnoë 1993). In all of these studies, little or no resistance was noted when transgenic plant lines were challenged with other potyviruses.…”

mentioning

confidence: 95%

Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVY^N coat protein gene

McDonald¹,

Brandle²,

Gleddie³

et al. 1997

Can. J. Plant Sci.

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. Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVY N coat protein gene. Can. J. Plant Sci. 77: 167-171. Pure breeding lines of transgenic Nicotiana tabacum L. 'Delgold' were generated which expressed transcripts of the coat protein gene of the tobacco veinal necrosis stain of potato virus Y (PVY N ) and were highly resistant to the virus. The form of resistance appeared to be RNA-mediated as no coat protein could be detected by enzyme linked immunosorbent assay. Resistant lines had two or three copies of the transgene but segregation analysis of the R 1 and R 2 progeny indicated that one copy in each line was functional for virus resistance. Simultaneous screening of lines for resistance against both PVY N and the heterologous PVY O (common) strain allowed for optimal selection of heterologous strain resistance. Resistance against the PVY-mn and PVY-nn strains and an ungrouped isolate, PVY-136, was less than against the PVY O isolate. The degree of heterologous strain resistance did not appear to directly correlate with the degree of sequence homology previously reported. The lines had no resistance against the related potato virus A and pepper mottle virus. Can. J. Soil Sci. 77: 167-171. On a obtenu des lignées généalogiques pures de tabac transgénique Nicotiana tabacum L. 'Delgold', exprimant les transcrits du gène de protéine capsidienne de la souche de la nécrose des nervures du tabac du PVY (PVY N ), mais manifestant une forte résistance au virus. Cette forme de résistance semble à médiation ARN, puisque aucune protéine capsidienne n'a pu être décelée par dosage immunoenzymatique. Les souches résistantes portaient deux ou trois copies du transgène, mais l'analyse de ségrégation des descendances R 1 et R 2 révélait qu'une copie de chaque souche conférait une résistance effective au virus. Des recherches simultanées de lignées dotées de résistance à la fois contre la souche homologue PVY N et contre la souche hétérologue PVY O (ordinaire) ouvraient la voie à une sélection optimale sur la résistance à la souche hétérologue. La résistance envers les souches PVY-mn et PVY-nn ainsi qu'envers un isolat non classifié, PVY-136, était moins forte que la résistance à la forme PVY O . Le degré de résistance à la souche hétérologue ne semble pas directement relié au degré d'homologie de séquence précédemment signalée. Les lignées ne démontraient pas de résistance au virus A de la pomme de terre ni au virus de la marbrure du piment, deux virus apparentés au PVY.

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“…This might result in transmission of the transcapsidated virus (which might be the transgenic form) in a new way or by a different vector (Lecoq et al, 1993 ;Robinson, 1996), possibly extending its host range (Harding, 1996). Transcapsidation of virus genomes by proteins from crops expressing viral transgenes has been demonstrated (Osbourn, Sarkar & Wilson, 1990 ;Farinelli, Malnoe & Collet, 1992 ;Candelier-Harvey & Hull, 1993 ;Lecoq et al, 1993), and there is a real possibility of viral transcapsidation in co-infected hosts.…”

Section:       mentioning

confidence: 99%

The release of genetically engineered micro‐organisms and viruses into the environment

Giddings

1998

New Phytologist

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This review considers the reasons for, and research governing, the regulation and monitoring of genetically engineered micro‐organisms and viruses (GEMs) released into the environment. The hazards associated with releasing GEMs into the environment are the creation and evolution of new pests and diseases, and damage to the ecosystem and non target species. The similarities and differences between GEMs and conventional micro‐organisms are discussed in relation to risk assessment. Other issues covered include the persistence of micro‐organisms in the environment, transgene dispersal to non‐engineered microbes and other organisms, the effects of transgenes and transformation on fitness, and the evolution of pests and pathogens that are given or acquire transgenes. Areas requiring further research are identified and recommendations for risk assessment made.

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Heterologous Encapsidation of Potato Virus Y Strain O (PVYO) With The Transgenic Coat Protein Of PVY Strain N (PVYN) In Solanum Tuberosum Cv. Bintje

Cited by 67 publications

References 29 publications

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVY^N coat protein gene

The release of genetically engineered micro‐organisms and viruses into the environment

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Heterologous Encapsidation of Potato Virus Y Strain O (PVYO) With The Transgenic Coat Protein Of PVY Strain N (PVYN) In Solanum Tuberosum Cv. Bintje

Cited by 67 publications

References 29 publications

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Transgenic accumulation of two plant virus coat proteins on a single self-processing polypeptide.

Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVYN coat protein gene

The release of genetically engineered micro‐organisms and viruses into the environment

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Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVY^N coat protein gene