Heterogeneity of NK-2 tachykinin receptors in hamster and rabbit smooth muscles

Maggi, Carlo Alberto; Eglezos, Anthony; Quartara, Laura; Patacchini, Riccardo; Giachetti, Antonio

doi:10.1016/0167-0115(92)90657-g

Cited by 36 publications

(12 citation statements)

References 15 publications

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“…A covariance analysis indicated a parallelism between the linear part of the dose-response curves for GLIC at four doses, for OXM at three doses, for OXM (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37) at four doses and for OXM (30)(31)(32)(33)(34)(35)(36)(37) at three doses. These parallels were significantly distinct (P = 0.001), except when data for OXM were compared to data for OXM (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37).…”

Section: Dose-response Curvesmentioning

confidence: 96%

“…To evaluate the importance of the C-terminal moiety, two C-terminal fragments active on gastric acid secretion, namely OXM(19-37) and OXM (30)(31)(32)(33)(34)(35)(36)(37), were also tested. OXM (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37) showed the same efficacy as the mother molecule (14.9 ± 1.1% decrease in cell length at 10 -7 M, n = 9) but was 14.5-fold less potent than GLIC and as potent as OXM (Fig. 3) (EC 50 = 72 pM).…”

Section: Distribution Of Cell Lengthmentioning

confidence: 97%

“…OXM (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37) and OXM (30)(31)(32)(33)(34)(35)(36)(37) were synthesized in our laboratory by the solid-phase method using chloromethylated resin and the Boc-HF strategy. The coupling reactions were carried out manually with the use of benzotriazolyl-oxy tris dimethyl aminophosphonium hexafluorophosphate (BOP) [22].…”

Section: Peptidesmentioning

confidence: 99%

“…This work has demonstrated a specific myogenic effect of these hormones. The structure-activity relationship was investigated using GLIC, OXM and the two Cterminal fragments, OXM (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37) and OXM(30-37).…”

Section: Introductionmentioning

confidence: 99%

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Effect of glicentin, oxyntomodulin and related peptides on isolated gastric smooth muscle cells

Rodier

Magous

Mochizuki

et al. 1997

Pfl�gers Archiv European Journal of Physiology

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Glicentin (proglucagon 1-69 GLIC) and oxyntomodulin (proglucagon 33-69 or OXM) are two peptide hormones that are co-released from ileum and large intestine during digestion. They modulate in vivo gastric acid secretion and the gastro-pyloro-duodenal activity. The specificity of their effects is linked to the presence of their C-terminal octapepide. As yet, no isolated target cell that responds specifically to this family of peptides has been described. The present report describes the in vitro effect of human synthetic GLIC, OXM and octapeptide-bearing fragments on smooth muscle cells isolated from the rabbit antrum. GLIC or OXM decreased the mean length of the cells by: 13.9 +/- 0.8% and 15.5 +/- 0.9%, respectively - GLIC being 16 times more potent than OXM (respective EC50 values: 5 and 83 pM). The C-terminal fragments OXM(19-37) and OXM(30-37) were as efficient as GLIC or OXM. Their potencies were OXM = OXM(19-37)>>OXM(30-37). Glucagon, which corresponds to OXM without the C-terminal octapeptide, or glucagon-like peptide-1 (7-36 amide) did not have any effect. The response to OXM was not influenced by antagonists to muscarinic, cholecystokinin or substance P receptors. In conclusion, our studies demonstrate for the first time an isolated target cell that responds specifically to GLIC, OXM and other octapeptide-bearing peptides.

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Section: Dose-response Curvesmentioning

confidence: 96%

Section: Distribution Of Cell Lengthmentioning

confidence: 97%

Section: Peptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of glicentin, oxyntomodulin and related peptides on isolated gastric smooth muscle cells

Rodier

Magous

Mochizuki

et al. 1997

Pfl�gers Archiv European Journal of Physiology

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“…In the airways, microvascular leakage, increased blood flow, vasodilatation, and to a less extent smooth muscle contraction are mediated by the tachykinin NK 1 receptors [4,9,10], whereas the tachykinin NK 2 receptors are the major mediators of tachykinin-induced airway smooth muscle contraction in animals and human [10]. In contrast, the tachykinin NK 3 receptors do not appear to play a prominent role in the direct regulation of airway smooth muscle tone [11][12][13], although they can mediate hyperresponsiveness [14][15][16][17].…”

mentioning

confidence: 98%

Bronchoconstrictor effect of the tachykinin NK₃-receptor agonists [MePhe⁷]-neurokinin B and senktide in the isolated guinea pig lung

Corboz¹,

Rivelli²,

Eckel³

2010

Experimental Lung Research

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To determine whether bronchoconstriction can be mediated via the tachykinin NK₃ receptors, isolated guinea pig lungs were challenged with the exogenous tachykinin NK₃-receptor agonists [MePhe⁷]-neurokinin B ([MePhe⁷]-NKB) and senktide. [MePhe⁷]-NKB induced bronchoconstriction (EC50 = 11.8 ± 1.7 µM) that was significantly inhibited by the tachykinin NK₃-receptor antagonist SB 223412 at 10 µM (EC50 = 24.4 ± 4.5 µM). Senktide also induced bronchoconstriction (EC50 = 96.2 ± 20.3 µM) and the bronchoconstriction was significantly reduced by SB 223412 at 1 and 10 µM (EC50 = 270.8 ± 78.9 µM and 388.3 ± 105.5 µM, respectively). Although the authors demonstrated that SB 223412, [MePhe⁷]-NKB, and senktide are potent and selective for the tachykinin NK3 receptors in binding and functional (Ca(2+) mobilization) assays, the tachykinin NK₁-receptor antagonist CP 99,994 at 1 µM (EC50 = 32.7 ± 8.5 µM) produced inhibition of [MePhe⁷]-NKB-induced bronchoconstriction, whereas the tachykinin NK₂-receptor antagonist SR 48968 at 0.1 µM (EC50 = 213.2 ± 42.9 µM) blocked senktide-induced bronchoconstriction. These data suggest that [MePhe⁷]-NKB and senktide caused bronchoconstriction in guinea pig through activation of the tachykinin NK₃-receptors but the tachykinin NK₁- and/or NK₂-receptors are also involved in the response.

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Evidence for Receptor Subtypes/Species Variants of Receptors

Maggi¹

1994

The Tachykinin Receptors

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Heterogeneity of NK-2 tachykinin receptors in hamster and rabbit smooth muscles

Cited by 36 publications

References 15 publications

Effect of glicentin, oxyntomodulin and related peptides on isolated gastric smooth muscle cells

Effect of glicentin, oxyntomodulin and related peptides on isolated gastric smooth muscle cells

Bronchoconstrictor effect of the tachykinin NK₃-receptor agonists [MePhe⁷]-neurokinin B and senktide in the isolated guinea pig lung

Evidence for Receptor Subtypes/Species Variants of Receptors

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Heterogeneity of NK-2 tachykinin receptors in hamster and rabbit smooth muscles

Cited by 36 publications

References 15 publications

Effect of glicentin, oxyntomodulin and related peptides on isolated gastric smooth muscle cells

Effect of glicentin, oxyntomodulin and related peptides on isolated gastric smooth muscle cells

Bronchoconstrictor effect of the tachykinin NK3-receptor agonists [MePhe7]-neurokinin B and senktide in the isolated guinea pig lung

Evidence for Receptor Subtypes/Species Variants of Receptors

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Bronchoconstrictor effect of the tachykinin NK₃-receptor agonists [MePhe⁷]-neurokinin B and senktide in the isolated guinea pig lung