Evidence for Receptor Subtypes/Species Variants of Receptors

Maggi, Carlo Alberto

doi:10.1007/978-1-4612-0301-8_14

Cited by 12 publications

(11 citation statements)

References 131 publications

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“…Alternatively, it is possible that the neurons express a variant of the NK 1 receptor that is not recognised by the antisera we have used. Indeed, there are different forms of the NK 1 receptor (Maggi, 1994), and one of them lacks much of the intracellular domain against which these antisera were raised. However, the overall distribution of NK 1 immunoreactivity in the sympathetic ganglia matches that seen with autoradiographic labelling of NK 1 binding sites (Messenger and Gibbins, 1998).…”

Section: Discussionmentioning

confidence: 99%

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

1999

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We have used multiple‐labelling immunohistochemistry and confocal microscopy to determine the distribution of immunoreactivity to the tachykinin neurokinin‐1 (NK1) receptors in guinea pig sympathetic ganglia. Although nerve fibres containing immunoreactivity to substance P were common in all ganglia except the superior cervical ganglia, most neurons expressing NK1 receptor immunoreactivity were not closely surrounded by pericellular baskets of substance P‐immunoreactive boutons. Conversely, many neurons surrounded by baskets of substance P‐immunoreactive boutons lacked NK1 immunoreactivity. In the coeliac and inferior mesenteric ganglia, NK1 receptor expression was restricted almost entirely to noradrenergic neurons that contained somatostatin immunoreactivity and projected to the enteric plexuses. In the lumbar chain and paracervical ganglia, NK1 immunoreactivity was expressed by nonnoradrenergic vasodilator neurons containing immunoreactivity to vasoactive intestinal peptide. Taken together, our results show that sympathetic neurons in different functional pathways express NK1 receptor immunoreactivity. However, the neurons that could respond to endogenously released substance P through NK1 receptors may be distant from presynaptic release sites. These observations suggest that, in sympathetic ganglia, substance P may modulate ganglionic transmission through heterosynaptic actions on NK1 receptors. J. Comp. Neurol. 412:693–704, 1999. © 1999 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

1999

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“…Another compound that can produce vasomotor responses is substance P (SP), one of the most widely expressed peptides in the CNS. Together with neurokinins A and B (NKA and NKB) it belongs to the tachykinin family, whose biological actions are mediated through G‐protein‐coupled tachykinin receptors designated NK1, NK2, and NK3 (Maggi, ; Harrison and Geppetti, ). The highest affinity of SP is for NK1 receptor (NK1 R ), whereas NKA and NKB have the highest affinity for NK2 and NK3 receptor, respectively (Maggi, ; Harrison and Geppetti, ).…”

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confidence: 99%

Intracallosal neuronal nitric oxide synthase neurons colocalize with neurokinin 1 substance P receptor in the rat

Barbaresi

Mensà

Lariccia

et al. 2014

J of Comparative Neurology

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The corpus callosum (cc) contains nitric oxide (NO)-producing neurons. Because NO is a potent vasodilator, these neurons could translate neuronal signals into vascular responses that can be detected by functional brain imaging. Substance P (SP), one of the most widely expressed peptides in the CNS, also produces vasomotor responses by inducing calcium release from intracellular stores through its preferred neurokinin 1 (NK1) receptor, thus inducing NO production via activation of neuronal NO synthase (nNOS). Single- and double-labeling experiments were performed to establish whether NK1-immunopositive neurons (NK1IP -n) are found in the rat cc and the extent of NK1 colocalization with nNOS. NK1IP -n were seen to constitute a large neuronal population in the cc and had a distribution similar to that of nNOSIP neurons (nNOSIP -n). NK1IP -n were numerous in the lateral cc and gradually decreased in the more medial portions, where they were few or absent. Intracallosal NK1IP -n and their dendritic trees were intensely labeled, allowing classification into four morphological types: bipolar, round, polygonal, and pyramidal. Confocal microscopic examination demonstrated that nearly all NK1IP -n contained nNOS (96.43%) and that 84.59% of nNOSIP -n co-expressed NK1. These data suggest that the majority of intracallosal neurons can release NO as a result of the action of SP. A small proportion of nNOSIP -n does not contain NK1 and is not activated by SP; these neurons may release NO via alternative mechanisms. The possible mechanisms by which intracallosal neurons release NO are also reviewed.

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“…Both GPI and RUB preparations were used to define the differential pharmacology at NK 1 receptors (Petitet et al, 1992;Meini et al, 1994). These differences were confirmed in various species (Maggi, 1994(Maggi, , 1995 Advenier et al (1992), Maggi et al (1993), Maggi, (1995), Patacchini et al (1995), Catalioto et al (1998), and Cialdai et al (2006). in, inactive up to μM concentrations; * , insurmountable antagonist behavior.…”

Section: Influence Of Pharmacological Heterogeneity On the Choice Of mentioning

confidence: 88%

Tachykinin Receptor Assays

Meini

Maggi

2010

CP Pharmacology

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Described in this unit are methods for obtaining, preparing, and testing smooth muscle preparations bearing tachykinin receptors to study the agonist or antagonist properties of test compounds. Concentration-response curves to agonists are constructed to measure their ability to produce smooth muscle contractions and thus evaluate the potency and efficacy of the agonists. Antagonists are tested for their ability to shift the agonist concentration-response curve and to calculate their potency. Two different protocols are described for each of the three tachykinin receptors (NK(1), NK(2), and NK(3)). The NK(1) receptor assays use guinea pig ileum longitudinal muscle myenteric plexus (GPI) and rat urinary bladder (RUB), the NK(2) receptor assays use isolated endothelium-deprived rabbit pulmonary artery (RPA) and hamster trachea (HT), and the NK(3) receptor assays use GPI and rat portal vein (RPV).

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Evidence for Receptor Subtypes/Species Variants of Receptors

Cited by 12 publications

References 131 publications

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

Neurokinin-1 receptor localisation in guinea pig autonomic ganglia

Intracallosal neuronal nitric oxide synthase neurons colocalize with neurokinin 1 substance P receptor in the rat

Tachykinin Receptor Assays

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