Heterogeneity of intrinsic plasticity in cerebellar Purkinje cells linked with cortical molecular zones

Nguyen-Minh, Viet T.; Wang, Tianzhuo; Tran-Anh, Khoa; Sugihara, Izumi

doi:10.1016/j.isci.2021.103705

Cited by 10 publications

(22 citation statements)

References 47 publications

(129 reference statements)

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“…Our findings are in line with previous studies on regional differences in LTD-induction 20 , 21 as well as regional and cellular differences in postsynaptic LTP-induction 22 , but it remains to be elucidated to what extent differences in presynaptic input versus intrinsic properties contribute to differences in the downbound or upbound nature of PCs. We therefore aimed to isolate the presynaptic element by stimulating PFs at a high frequency in coronal slices at room temperature, which is known to increase the primary effector of presynaptic LTP, nitrogen monoxide 23 (Fig.…”

Section: Resultssupporting

confidence: 92%

Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity

et al. 2022

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Cerebellar learning is expressed as upbound or downbound changes in simple spike activity of Purkinje cell subpopulations, but the underlying mechanism remains enigmatic. By visualizing murine Purkinje cells with different molecular identities, we demonstrate that the potential for induction of long-term depression is prominent in downbound and minimal in the upbound subpopulation. These differential propensities depend on the expression profile, but not on the synaptic inputs, of the individual Purkinje cell involved, highlighting the functional relevance of intrinsic properties for memory formation.

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Section: Resultssupporting

confidence: 92%

Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity

et al. 2022

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“…Mouse strains in which zebrin stripes are intrinsically visualized are useful for this purpose. So far, EAAT4‐EGFP mice (Voerman et al., 2022), Aldoc‐Venus mice (Fujita et al., 2014), and Rgs8‐EGFP mice (Viet et al., 2021) have been used for this purpose. The two latter strains were used in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, we aimed to clarify the hemispheric lobule‐related topographic subdivisional organization in the lPIN and the LN and the principal olive (PO) and the rMAO in mice. For this purpose, we used Aldoc‐Venus and RgS8‐EGFP mice (Fujita et al., 2014; Viet et al., 2021), in which zebrin stripes are visualized, to inject bidirectional tracers into various lobules and stripes in the cerebellar hemisphere and paravermis. The three‐dimensional (3D) special organization of the distributions of labeled axonal terminals and neurons was analyzed in the CN and IO to understand the lobular representation systematically.…”

Section: Introductionmentioning

confidence: 99%

Topographic organization in the cerebellar nuclei and inferior olive in relation to cerebellar hemispheric lobules in the mouse: Distinction between crus I and neighboring lobules

Owusu-Mensah

Sarpong

Sugihara

2023

J of Comparative Neurology

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The parallel closed‐loop topographic connections between subareas of the inferior olive (IO), cerebellar cortex, and cerebellar nuclei (CN) define the fundamental modular organization of the cerebellum. The cortical modules or zones are organized into longitudinal zebrin stripes which are extended across transverse cerebellar lobules. However, how cerebellar lobules, which are related to the cerebellar functional localization, are incorporated into the olivo‐cortico‐nuclear topographic organization has not been fully clarified. In the present study, we analyzed the lobular topography in the CN and IO by making 57 small bidirectional tracer injections in the lateral zebrin‐positive stripes equivalent with C2, D1, and D2 zones in every hemispheric lobule in zebrin stripe‐visualized mice.C2, D1, and D2 zones were connected to the lateral part of the posterior interpositus nucleus (lPIN), and caudal and rostral parts of the lateral nucleus (cLN, rLN), respectively, and from the rostral part of the medial accessory olive (rMAO), and ventral and dorsal lamellas of the PO (vPO, dPO), respectively, as reported. Within these areas, crus I was specifically connected to the ventral parts of the lPIN, cLN, and rLN, and from the rostrolateral part of the rMAO and the lateral parts of the vPO and dPO.The results indicated that the cerebellar modules have lobule‐related subdivisions and that crus I is topographically distinct from other lobules. We speculate that crus I and crus I‐connected subdivisions in the CN and IO are involved more in nonmotor functions than other neighboring areas in the mouse.

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“…In the area that we targeted, the vermal lobule VI, most or all Purkinje cells express the molecular marker zebrin II ( Cerminara et al, 2015 ). Zebrin positive and zebrin negative Purkinje cells differ in many structural and functional parameters, from the dominant type of climbing fiber input that they receive to the level of intrinsic excitability and spiking activity ( Zhou et al, 2014 ; Cerminara et al, 2015 ; Viet et al, 2022 ). The extent to which the current results extend to zebrin negative Purkinje cells is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The extent to which the current results extend to zebrin negative Purkinje cells is unknown. On the other hand, zebrin negative cells express stronger intrinsic and synaptic plasticity relative to zebrin positive cells ( Wadiche and Jahr, 2005 ; Viet et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural effects of sleep and wake on the parallel fiber synapses of the cerebellum

Loschky

Spano

Marshall

et al. 2022

eLife

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Multiple evidence in rodents shows that the strength of excitatory synapses in the cerebral cortex and hippocampus is greater after wake than after sleep. The widespread synaptic weakening afforded by sleep is believed to keep the cost of synaptic activity under control, promote memory consolidation, and prevent synaptic saturation, thus preserving the brain’s ability to learn day after day. The cerebellum is highly plastic and the Purkinje cells, the sole output neurons of the cerebellar cortex, are endowed with a staggering number of excitatory parallel fiber synapses. However, whether these synapses are affected by sleep and wake is unknown. Here, we used serial block face scanning electron microscopy to obtain the full 3D reconstruction of more than 7000 spines and their parallel fiber synapses in the mouse posterior vermis. This analysis was done in mice whose cortical and hippocampal synapses were previously measured, revealing that average synaptic size was lower after sleep compared to wake with no major changes in synapse number. Here, instead, we find that while the average size of parallel fiber synapses does not change, the number of branched synapses is reduced in half after sleep compared to after wake, corresponding to ~16% of all spines after wake and ~8% after sleep. Branched synapses are harbored by two or more spines sharing the same neck and, as also shown here, are almost always contacted by different parallel fibers. These findings suggest that during wake, coincidences of firing over parallel fibers may translate into the formation of synapses converging on the same branched spine, which may be especially effective in driving Purkinje cells to fire. By contrast, sleep may promote the off-line pruning of branched synapses that were formed due to spurious coincidences.

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Heterogeneity of intrinsic plasticity in cerebellar Purkinje cells linked with cortical molecular zones

Cited by 10 publications

References 47 publications

Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity

Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity

Topographic organization in the cerebellar nuclei and inferior olive in relation to cerebellar hemispheric lobules in the mouse: Distinction between crus I and neighboring lobules

Ultrastructural effects of sleep and wake on the parallel fiber synapses of the cerebellum

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