Heterogeneity of <i>Streptomyces</i> DNA

Antonov, P.; Ivanov, Iván; Марков, Георги

doi:10.1016/0014-5793(77)80372-4

Cited by 18 publications

(6 citation statements)

References 11 publications

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“…Our results for the differences in the denaturation velocities of the native and PMSinhibited mesentericopeptidase are in good agreement with those obtained for cuchymotrypsin. It was demonstrated that NE-methylhistidine-57a-chymotrypsin is denaturated faster than the native enzyme (Antonov Vorotyntseva, 1972). There are wide differences in the biological origin of the two above men-188 tioned enzymes, but they are both serine proteases and probably possess similar hydrogen bond networks in their active sites.…”

Section: Resultsmentioning

confidence: 99%

CIRCULAR DICHROISM STUDIES ON NATIVE AND PHENYLMETHANESULFONYL‐MESENTERICOPEPTIDASE

Genov

Shopova

1978

International Journal of Peptide and Protein Research

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At neutral pH the far ultraviolet circular dichroism (CD) spectrum of alkaline mesentericopeptidase is dominated by two negative bands: at 208 and 220 nm. The near ultraviolet CD spectrum is characterized by a large negative band at 277nm, a shoulder near 286nm and a small positive band at 297nm. The introduction of phenylmethanesulfonyl (PMS) group on the serine residue at the active site does not alter the intensity and the wavelength position of the bands. In contrast to the other alkaline bacterial proteases, which are remarkably resistant to denaturation by urea, mesentericopeptidase was unfolded in the presence of this reagent at neutral pH. The denaturation reactions in the presence of 9.5 M urea of both native and PMS‐mesentericopeptidase followed first order kinetics. The introduction of PMS‐group on the active‐site serine lowered the conformational stability of the enzyme. In the first 3h PMS‐mesentericopeptidase was denaturated 2.5 times faster than native protease. In the presence of 9.5 M urea at natural pH alkaline mesentericopeptidase lost its catalytic activity. The loss of enzymatic activity correlated with the unfolding of the protein molecule and also followed first‐order kinetics.

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Section: Resultsmentioning

confidence: 99%

CIRCULAR DICHROISM STUDIES ON NATIVE AND PHENYLMETHANESULFONYL‐MESENTERICOPEPTIDASE

Genov

Shopova

1978

International Journal of Peptide and Protein Research

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“…The reassociation profile of S. coelicolor DNA suggests the presence of repeated sequences (2,7). Examples of highly amplified (reiterated) DNA sequences have been reported in several members of the genus Streptomyces (15,35,36,(38)(39)(40).…”

Section: Resultsmentioning

confidence: 95%

“…Caulobacter crescentus has been reported to have enough repeat sequences to show up in a Cot analysis (49). Moreover, Antonov et al (2) have shown, by using Cot data as well as other methods, that S. coelicolor contains about 5% reiterated DNA. Our data further indicate that reiterated sequences in streptomycetes may be measurable by using Cot analyses.…”

Section: Resultsmentioning

confidence: 99%

Determination of the molecular mass of bacterial genomic DNA and plasmid copy number by high-pressure liquid chromatography

Genthner¹,

Hook²,

Strohl³

1985

Appl Environ Microbiol

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Relatively rapid methods for the determination of relative genome molecular mass (Mr) and the estimation of plasmid copy number have been developed. These methods are based on the ability of the Bio-Rad high-pressure liquid chromatography hydroxylapatite column to separate and quantify single-stranded DNA, double-stranded DNA, and plasmid DNA. Genome Mr values were calculated from reassociation kinetics of single-stranded DNA as measured with the hydroxylapatite column. Bacteriophage T4 DNA was used to establish a Cot (moles of nucleotides times seconds per liter), or standard reassociation value. From this Cot value, Cot values for Escherichia coli B, Beggiatoa alba B18LD, and Streptomyces coelicolor were determined by comparative calculations. From those calculated Cot values, the Mr values of 1.96 x 109 for E. coli, 2.02 x 109 for B. alba, and 3.28 x 109 for S. coelicolor were estimated. Plasmid concentration was determined from

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“…Thus, it must be concluded that the band is heterogeneous. A candidate for the origin of this satellite DNA is the reiterated sequences shown to make up almost 10% of the chromosomal DNA of, for example, S. coelicolor [9].…”

Section: S Virginiaementioning

confidence: 99%

A survey of Streptomyces species for covalently closed circular (ccc) DNA using a variety of methods

Kirby

1982

FEMS Microbiology Letters

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Heterogeneity of Streptomyces DNA

Cited by 18 publications

References 11 publications

CIRCULAR DICHROISM STUDIES ON NATIVE AND PHENYLMETHANESULFONYL‐MESENTERICOPEPTIDASE

CIRCULAR DICHROISM STUDIES ON NATIVE AND PHENYLMETHANESULFONYL‐MESENTERICOPEPTIDASE

Determination of the molecular mass of bacterial genomic DNA and plasmid copy number by high-pressure liquid chromatography

A survey of Streptomyces species for covalently closed circular (ccc) DNA using a variety of methods

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